Zobrazeno 1 - 10
of 45
pro vyhledávání: '"Happy K, Shieh"'
Publikováno v:
Taiwan Veterinary Journal. 40:173-180
Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals and the outbreak of this disease may result in a severe economic loss. The spread of FMD is controlled by measures including movement restriction, stamping-out polic
Publikováno v:
Journal of Veterinary Diagnostic Investigation. 23:543-546
The lipopolysaccharide, also known as the somatic antigen or O-antigen, is an important virulence factor of Pasteurella multocida. In the current study, the genes involved in the biosynthesis of the outer core region of the lipopolysaccharide, which
Autor:
Happy K. Shieh, Jui-Hung Shien, Ching-Lin Shyu, Poa-Chun Chang, Ping-Yi Chen, Jin-Ru Wu, Fanny Chang
Publikováno v:
Veterinary Microbiology. 145:90-99
The aim of this study was to investigate biosynthesis genes and chemical components of the capsule of Avibacterium paragallinarum. The sequence of a 10-kb region containing the capsule biosynthetic locus of Av. paragallinarum was determined. Two refe
Autor:
Happy K. Shieh, Ming-Chung Deng, J.Z. Shiau, Yeou-Liang Lin, Chia-Yi Chang, Chin-Cheng Huang, Ming-Shiuh Lee
Publikováno v:
Veterinary Microbiology. 124:193-201
In 2003, an avian influenza (AI) virus of H5N1 subtype (A/Duck/China/E319-2/03; Dk/CHN/E319-2/03) was isolated from a smuggled duck in Kinmen Island of Taiwan. Phylogenetic analysis and pairwise comparison of nucleotide and amino acid sequences revea
Publikováno v:
Veterinary Microbiology. 122:280-289
Inactivated vaccines of Avibacterium paragallinarum provide protection and reduce the economic losses caused by infectious coryza. However, inactivated bacterins provide protection only against the Page serovars included in the vaccine. In this study
Autor:
Poa-Chun Chang, Chung-Chi Hu, Jin-Ru Wu, Jui-Hung Shien, Shuen-Rong Gong, Ling-Yun Chen, Happy K. Shieh
Publikováno v:
FEMS Microbiology Letters. 267:113-120
We have identified a new phoX gene encoding the monomeric alkaline phosphatase from Pasteurella multocida X-73. This gene was not found in the published genome sequence of Pasteurella multocida pm70. Characterization of the recombinant PhoX of Pasteu
Autor:
Long-Huw Lee, Yung-Sheng Chi, Kuan-Chih Chow, Happy K. Shieh, Yi-Hsin Fan, Jui-Hung Shien, Chun-Da Lin, Shiow-Her Chiou
Publikováno v:
Veterinary Immunology and Immunopathology. 114:49-60
To identify cell types and genes that are differentially expressed during immunopathogenesis of avian reovirus (ARV)-induced viral arthritis (VA), we inoculated arthrotropic strain S1133 of ARV into 1-day-old broilers, and examined tissue histology a
Autor:
Ya-Li Wang, Hsiao-Li Tu, You Chan, Yu-Tsai Chi, Wu-Tse Liu, Happy-K Shieh, Keh-Liang Lin, Chi-Ho Chan
Publikováno v:
Journal of Virological Methods. 136:38-43
This study describes the development of a simple RT-PCR method to amplify the whole genome of the influenza A virus based on the amplification of full-length gene segments. Primers were designed based on the conserved regions of both the 5'-end and t
Autor:
Happy K. Shieh, Yu C. Hu, Hung J. Liu, Yu L. Luo, Poa C. Chang, Julius L.C. Chulu, Chi Y. Wang, Wen T. Ji
Publikováno v:
Journal of Virological Methods. 135:43-48
Baculovirus/insect cell system is used widely for recombinant protein production. The hemagglutinin (HA) gene of H5N2 avian influenza virus (AIV) 1209 strain and the enhanced green fluorescent protein (EGFP) gene were cloned into pFastBac DUAL vector