Zobrazeno 1 - 10
of 59
pro vyhledávání: '"Haoquan Wu"'
Publikováno v:
Frontiers in Microbiology, Vol 9 (2018)
MicroRNAs (miRNAs) were first identified in Caenorhabditis briggsae and later recognized as playing pivotal roles in a vast range of cellular activities. It has been shown that miRNAs are an important mechanism not only for host defense against virus
Externí odkaz:
https://doaj.org/article/d3b5099e470d49eead5bd0f9dd5eaa79
Autor:
Hongming Ma, Ying Dang, Yonggan Wu, Gengxiang Jia, Edgar Anaya, Junli Zhang, Sojan Abraham, Jang-Gi Choi, Guojun Shi, Ling Qi, N. Manjunath, Haoquan Wu
Publikováno v:
Cell Reports, Vol 12, Iss 4, Pp 673-683 (2015)
West Nile virus (WNV) causes an acute neurological infection attended by massive neuronal cell death. However, the mechanism(s) behind the virus-induced cell death is poorly understood. Using a library containing 77,406 sgRNAs targeting 20,121 genes,
Externí odkaz:
https://doaj.org/article/db801ee050da440190b4ff28a57f02c8
Publikováno v:
Heliyon, Vol 2, Iss 9 (2016)
5′-isomiRs expand the repertoire of miRNA targets. However, how they are generated is not well understood. Previously, we showed that for some miRNAs in mammalian cells, Drosha cleaves at multiple sites to generate multiple pre-miRNAs that give ris
Externí odkaz:
https://doaj.org/article/a7b2f7e04e524861bd065d9999fbca66
Autor:
Qingwen Jin, Hong Chen, Xingxia Wang, Liandong Zhao, Qingchen Xu, Huijuan Wang, Guanyu Li, Xiaofan Yang, Hongming Ma, Haoquan Wu, Xiaohui Ji
Publikováno v:
PLoS ONE, Vol 10, Iss 7, p e0131894 (2015)
Insertion of T4 lysozyme (T4L) into the GPCR successfully enhanced GPCR protein stability and solubilization. However, the biological functions of the recombinant GPCR protein have not been analyzed.We engineered the CCR5-T4L mutant and expressed and
Externí odkaz:
https://doaj.org/article/0c9906c3c75a4fcf82673df040ab0cc7
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 3, Iss C (2014)
Relatively large amounts of transfected siRNA can compete for Ago proteins and thus compromise endogenous miRNA function, potentially leading to toxicities. Here, we show that shRNA can also perturb endogenous miRNA function similarly. More important
Externí odkaz:
https://doaj.org/article/b25285ffd1734cfd9edfb64268b7beec
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 3, Iss C (2014)
Pol III promoters such as U6 are commonly used to express small RNAs, including small interfering RNA, short hairpin RNA, and guide RNA, for the clustered regularly interspaced short palindromic repeats genome-editing system. However, whether the sma
Externí odkaz:
https://doaj.org/article/b330abd3999247568f8a0877907de549
Publikováno v:
PLoS ONE, Vol 6, Iss 3, p e17889 (2011)
West Nile (WN) and St. Louis encephalitis (SLE) viruses can cause fatal neurological infection and currently there is neither a specific treatment nor an approved vaccine for these infections. In our earlier studies, we have reported that siRNAs can
Externí odkaz:
https://doaj.org/article/9e408482614e4fdf9cc1f803e1ad1616
Autor:
Haoquan Wu, Hongming Ma, Chunting Ye, Danielle Ramirez, Shuiping Chen, Jessica Montoya, Premlata Shankar, Xiaozhong A Wang, N Manjunath
Publikováno v:
PLoS ONE, Vol 6, Iss 12, p e28580 (2011)
siRNA (small interfering RNA) and shRNA (small hairpin RNA) are powerful and commonly used tools in biomedical research. Currently, siRNAs are generally designed as two 21 nt strands of RNA that include a 19 nt completely complementary part and a 2 n
Externí odkaz:
https://doaj.org/article/c2e001e514384726a836f59674a55c66
Autor:
Shuiping Chen, Harendra S Chahar, Sojan Abraham, Haoquan Wu, Theodore C Pierson, Xiaozhong A Wang, N Manjunath
Publikováno v:
PLoS ONE, Vol 6, Iss 11, p e27551 (2011)
RNA interference can be mediated by fully complementary siRNA or partially complementary miRNA. siRNAs are widely used to suppress viral replication and the fully complementary siRNA bound Ago-2 in the RISC is known to degrade the target RNA. Althoug
Externí odkaz:
https://doaj.org/article/6656b7c0b7054518aacc09978e79ea87
Publikováno v:
PLoS ONE, Vol 4, Iss 10, p e7566 (2009)
It is generally believed that the miRNA processing machinery ensures the generation of a mature miRNA with a fixed sequence, particularly at its 5' end. However, we and others have recently noted that the ends of a given mature miRNA are not absolute
Externí odkaz:
https://doaj.org/article/87ecc0a08d5a4a86b1b6076050067cc2