Zobrazeno 1 - 10
of 33
pro vyhledávání: '"Hanspeter Michel"'
Autor:
Kurt Hollfelder, Charles Belunis, Robert Crowther, Pete William Dunten, David J. Weber, Ursula Kammlott, Wayne Levin, Hanspeter Michel, Gwendolyn Ramsey, Amy Swain, Stanley Wertheimer
Publikováno v:
Journal of Molecular Biology. 316:257-264
We report crystal structures of the human enzyme phosphoenolpyruvate carboxykinase (PEPCK) with and without bound substrates. These structures are the first to be determined for a GTP-dependent PEPCK, and provide the first view of a novel GTP-binding
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b36444f143990f5f5c3214390f6d95e6
https://doi.org/10.1006/jmbi.2001.5364
https://doi.org/10.1006/jmbi.2001.5364
Autor:
Marek Sochacki, James Ofengand, Joan S. Tscherne, Paul Popienick, Hanspeter Michel, Kelvin Nurse
Publikováno v:
Biochemistry. 38:1884-1892
The methyltransferase that forms m5C967 in Escherichia coli small subunit ribosomal RNA has been purified, cloned, and characterized. The gene was identified from the N-terminal sequence of the purified enzyme. The gene is a fusion of two open readin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b678173bbdddbf1446a823f87edc504
https://doi.org/10.1021/bi981880l
https://doi.org/10.1021/bi981880l
Autor:
Kurt Hollfelder, Mary C. Graves, Seth P. Monkarsh, Pascal Bailon, Palleroni Alicia Vallejo, Emil A. Russoman, Jill E. Porter, Yuemei Ma, Yu-Ching E. Pan, Doreen Ciolek, Hanspeter Michel, Swapan K. Roy, Barabara Debarbieri, Anthony Aglione
Publikováno v:
Analytical Biochemistry. 247:434-440
The success of recombinant interferon alpha in the clinic in part is limited by two properties of the protein: short serum half-life and immunogenicity. To improve these properties, interferon alpha-2a was conjugated with polyethylene glycol (PEG-500
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::246368842f1b7396edb4e8997684a7bc
https://doi.org/10.1006/abio.1997.2128
https://doi.org/10.1006/abio.1997.2128
Autor:
Anish Konkar, William Riboulet, Barbara Lueckel, George Ehrlich, Waleed Danho, Petar Pop-Damkov, Karin Conde-Knape, Dominik Hainzl, Farooq Qureshi, Petra Goelzer, Hanspeter Michel, Theresa Truitt
Publikováno v:
Bioconjugate chemistry. 24(12)
Recombinant human serum albumin (HSA) conjugates of a 15-amino-acid truncated peptide YY (PYY) analogue were prepared using three heterobifunctional linkers [succinimidyl 4-[N-maleimidomethyl]cyclohexane-1-carboxylate (SMCC), 6-maleimidohexanoic acid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c0b3dc7fe745cb53ad2dda7722277fe
https://pubmed.ncbi.nlm.nih.gov/24251972
https://pubmed.ncbi.nlm.nih.gov/24251972
Publikováno v:
Gene. 169:59-64
A versatile plasmid vector was designed to direct the synthesis of recombinant proteins in either one of two forms that will be biotinylated in Escherichia coli with high efficiency at a single, unique site. The protein of interest can be produced wi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f14297c6a579f3f8d706a536fdfaae7
https://doi.org/10.1016/0378-1119(95)00762-8
https://doi.org/10.1016/0378-1119(95)00762-8
Autor:
Donald F. Hunt, Jeffrey Shabanowitz, John Hempel, Roger W. Hendrix, Hanspeter Michel, Robert L. Duda
Publikováno v:
Journal of Molecular Biology. 247:618-635
Bacteriophage HK97 builds its head shell from a 42 kDa major head protein, but neither this 42 kDa protein nor its processed, 31 kDa form is found in the mature head. Instead, each of the major head-protein subunits is covalently cross-linked into ol
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::29c1ac2e61ce65804347b31d7c0d07b2
https://doi.org/10.1016/s0022-2836(05)80143-3
https://doi.org/10.1016/s0022-2836(05)80143-3
Purification and characterization of a high-molecular-weight form of recombinant human Interleukin-2
Publikováno v:
Journal of Protein Chemistry. 13:591-598
During purification of recombinant Interleukin-2 (rIL-2) by reversed-phase HPLC, early fractions are discarded due to the presence of an unidentified form of rIL-2. A procedure has been developed to isolate and purify this unidentified form of rIL-2.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d21d11b88cecef45871749e54eb3935
https://doi.org/10.1007/bf01890457
https://doi.org/10.1007/bf01890457
Autor:
Hanspeter Michel, Ashley D. M. Wallace-Cook, Alison G. Smith, Jefferey Shabanowitz, Donald F. Hunt, Michael P. Timko, Huguette Albrecht, Anthony J. Spano, Michael Witty
Publikováno v:
Plant Physiology. 103:139-147
Porphobilinogen (PBG) deaminase catalyzes the polymerization of four PBG monopyrrole units into the linear tetrapyrrole hydroxymethylbilane necessary for the formation of chlorophyll and heme in plant cells. Degenerate oligonucleotide primers were de
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6135d551ff0c02c2285e5eb6857153d6
https://doi.org/10.1104/pp.103.1.139
https://doi.org/10.1104/pp.103.1.139
Autor:
Robert H. Kretsinger, Hanspeter Michel, Donald F. Hunt, Kenneth G. Pote, Jeffrey Shabanowitz, Charles R. Hauer
Publikováno v:
Biochemistry. 32:5017-5024
Otoconia are composites of proteins and inorganic crystals formed in the peripheral portion of the vestibular system of vertebrates. They add mass to the extracellular otoconial membrane, thereby increasing its deflection during linear acceleration.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4629c9f1fb080f060ada0af953bb4bee
https://doi.org/10.1021/bi00070a007
https://doi.org/10.1021/bi00070a007
Autor:
Hanspeter Michel, Kevin R. Lynch, Thomas W. Sturgill, Donald F. Hunt, Claire Haystead, Jeffrey K. Harrison, Timothy A.J. Haystead, Jie Wu, Leigh Ann Vincent
Publikováno v:
Proceedings of the National Academy of Sciences. 90:173-177
MAP kinases p42mapk and p44mapk participate in a protein kinase cascade(s) important for signaling in many cell types and contexts. Both MAP kinases are activated in vitro by MAP kinase kinase, a protein-tyrosine and threonine kinase. A MAP kinase ki
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b06d8de99276c0e2a918f48cc6be6f6
https://doi.org/10.1073/pnas.90.1.173
https://doi.org/10.1073/pnas.90.1.173