Zobrazeno 1 - 10
of 75
pro vyhledávání: '"Hansgeorg Schindler"'
Autor:
Markus Axmann, Kostya Kandror, John C. Roder, Hansgeorg Schindler, Andreas Jeromin, Susanne Freudenthaler, Gerhard J. Schütz
Publikováno v:
Microscopy Research and Technique. 63:159-167
Trafficking of secretory vesicles along neurites of PC12 cells was visualized by 2D and 3D real-time imaging using fluorescence microscopy. Vesicle motion along distinct pathways was directly seen. From an overlay of individual pathways, the underlyi
Autor:
Gerhard J. Schütz, Hana Borsukova, Alfred Böttcher, Hansgeorg Schindler, Gerd Schmitz, Gerhard Liebisch, Wolfgang Drobnik, Alexandra Pfeiffer
Publikováno v:
Traffic. 3:268-278
We have investigated whether a raft heterogeneity exists in human monocyte-derived macrophages and fibroblasts and whether these microdomains are modulated by lipid efflux. Triton X-100 (Triton) or Lubrol WX (Lubrol) detergent-resistant membranes fro
Autor:
Christian K. Riener, Hermann J. Gruber, Gerald Kada, Peter Hinterdorfer, Ferry Kienberger, Hansgeorg Schindler, Cordula Borken
Publikováno v:
Colloids and Surfaces B: Biointerfaces. 23:115-123
A fundamental prerequisite for the investigation of biological specimen by force microscopy is their immobilization to inert surfaces. In this review, we report recent anchoring protocols and their application for atomic force microscopy single molec
Autor:
Günter Freudenthaler, Hans-Günther Knaus, Hansgeorg Schindler, Gerhard J. Schütz, Bernt Pragl, Markus Axmann
Publikováno v:
Histochemistry and Cell Biology. 117:197-202
The determination of pharmacologically relevant constants is crucial in order to understand the effects of compounds interacting with various membrane receptors. In this report we study a venom component of the Central American scorpion Centruroides
Publikováno v:
Single Molecules. 2:99-103
We used dynamic force microscopy to study the attachment of human rhinovirus 2 (HRV2) particles to model membranes. A supported lipid bilayer containing Ni2+-nitrilotriacetate (NTA)-lipids as functional groups in the outer leaflet at high surface den
Publikováno v:
ResearcherID
One original impetus for the development of single molecule microscopy was its application to cell biology. Recently, several groups succeeded in the first demonstration of single molecule sensitivity in living biological cells. Still, these pioneeri
Autor:
Hansgeorg Schindler, Vassili Pastushenko, Peter Hinterdorfer, Gerald Kada, Ferry Kienberger, Christian K. Riener, Hermann J. Gruber
Publikováno v:
Single Molecules. 1:123-128
Molecular recognition force spectroscopy was employed to probe the mechanical and dynamical features poly(ethylene glycol) (PEG). His6 was covalently coupled to AFM tips via PEG for the specific recognition of NTA on the surface. Force-extension prof
Autor:
Bernt Pragl, Vassili Ph. Pastushenkoo, Jan Hesse, Gerhard J. Schütz, Günter Freudenthaler, Hans-G nter Knaus, Hansgeorg Schindler
Publikováno v:
Single Molecules. 1:153-157
Autor:
Hermann J. Gruber, Christian K. Riener, Gerald Kada, Peter Hinterdorfer, Susanne Wielert-Badt, Hansgeorg Schindler, Cordula Borken, Werner Baumgartner, Ferry Kienberger, Anneliese Raab
Publikováno v:
Single Molecules. 1:99-103
In this review, we report recent molecular recognition studies of our group. The surface chemistry by which ligands are covalently coupled to force microscopy tips is described. Poly(ethylene glycol), which is used as spacer for the ligands, was show
Autor:
Gerhard J. Schütz, Bernt Pragl, Vassili Pastushenko, Hermann J. Gruber, Hansgeorg Schindler, Hans-Günter Knaus
Publikováno v:
Single Molecules. 1:25-31
High affinity binding of fluorescence labeled hongotoxin (HgTX1-Cy5) to the potassium channel KV1.3 in T-lymphocyte cell membranes was utilized for imaging single ion channels optically, employing Single Dye Tracing (SDT). Binding sites were seen as