Protocol for simultaneous detection of mRNAs and (phospho-)proteins with ARTseq-FISH in mouse embryonic stem cells.

Autor: Hu X; Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, the Netherlands; Oncode Institute, Nijmegen, the Netherlands., van Sluijs B; Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, the Netherlands., García-Blay Ó; Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, the Netherlands; Oncode Institute, Nijmegen, the Netherlands., Huck WTS; Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, the Netherlands. Electronic address: w.huck@science.ru.nl., Hansen MMK; Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, the Netherlands; Oncode Institute, Nijmegen, the Netherlands. Electronic address: maike.hansen@ru.nl.

Publikováno v: STAR protocols [STAR Protoc] 2024 Oct 01; Vol. 5 (4), pp. 103336. Date of Electronic Publication: 2024 Oct 01.

Decoupled degradation and translation enables noise modulation by poly(A) tails.

Autor: Grandi C; Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, the Netherlands; Oncode Institute, Nijmegen, the Netherlands., Emmaneel M; Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, the Netherlands; Oncode Institute, Nijmegen, the Netherlands., Nelissen FHT; Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, the Netherlands; Oncode Institute, Nijmegen, the Netherlands., Roosenboom LWM; Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, the Netherlands., Petrova Y; Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, the Netherlands., Elzokla O; Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, the Netherlands., Hansen MMK; Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, the Netherlands; Oncode Institute, Nijmegen, the Netherlands. Electronic address: maike.hansen@ru.nl.

Publikováno v: Cell systems [Cell Syst] 2024 Jun 19; Vol. 15 (6), pp. 526-543.e7.

Proteomic analysis of SARS-CoV-2 particles unveils a key role of G3BP proteins in viral assembly.

Autor: Murigneux E; Université Paris Cité, CNRS, Inserm, Institut Cochin, F-75014, Paris, France., Softic L; Université Paris Cité, CNRS, Inserm, Institut Cochin, F-75014, Paris, France., Aubé C; Université Paris Cité, CNRS, Inserm, Institut Cochin, F-75014, Paris, France., Grandi C; Institute for Molecules and Materials, Radboud University, 6525, AJ, Nijmegen, the Netherlands., Judith D; Université Paris Cité, CNRS, Inserm, Institut Cochin, F-75014, Paris, France., Bruce J; Proteom'IC facility, Université Paris Cité, CNRS, Inserm, Institut Cochin, F-75014, Paris, France., Le Gall M; Proteom'IC facility, Université Paris Cité, CNRS, Inserm, Institut Cochin, F-75014, Paris, France., Guillonneau F; Proteom'IC facility, Université Paris Cité, CNRS, Inserm, Institut Cochin, F-75014, Paris, France.; Institut de Cancérologie de l'Ouest (ICO), CRCi2NA-Inserm UMR 1307, CNRS UMR 6075, Nantes Université, Angers, France., Schmitt A; Université Paris Cité, CNRS, Inserm, Institut Cochin, F-75014, Paris, France., Parissi V; Microbiologie Fondamentale et Pathogénicité Laboratory (MFP), UMR 5234, « Mobility of pathogenic genomes and chromatin dynamics » team (MobilVIR), CNRS-University of Bordeaux, DyNAVIR network, Bordeaux, France., Berlioz-Torrent C; Université Paris Cité, CNRS, Inserm, Institut Cochin, F-75014, Paris, France., Meertens L; Université Paris Cité, Inserm U944, CNRS 7212, Institut de Recherche Saint-Louis, Hôpital Saint-Louis, Paris, France., Hansen MMK; Institute for Molecules and Materials, Radboud University, 6525, AJ, Nijmegen, the Netherlands., Gallois-Montbrun S; Université Paris Cité, CNRS, Inserm, Institut Cochin, F-75014, Paris, France. sarah.gallois-montbrun@inserm.fr.

Publikováno v: Nature communications [Nat Commun] 2024 Jan 20; Vol. 15 (1), pp. 640. Date of Electronic Publication: 2024 Jan 20.

Noise Minimization in Cell-Free Gene Expression.

Autor: Bartelds MW; Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands., García-Blay Ó; Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands., Verhagen PGA; Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands., Wubbolts EJ; Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands., van Sluijs B; Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands., Heus HA; Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands., de Greef TFA; Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.; Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands.; Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands.; Computational Biology Group, Department of Biomedical Engineering, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands.; Center for Living Technologies, Eindhoven-Wageningen-Utrecht Alliance, 5600 MB Eindhoven, The Netherlands., Huck WTS; Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands., Hansen MMK; Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.

Publikováno v: ACS synthetic biology [ACS Synth Biol] 2023 Aug 18; Vol. 12 (8), pp. 2217-2225. Date of Electronic Publication: 2023 Jul 21.

A DNA repair pathway can regulate transcriptional noise to promote cell fate transitions.

Autor: Desai RV; Gladstone/UCSF Center for Cell Circuitry, Gladstone Institutes, San Francisco, CA 94158, USA.; Medical Scientist Training Program and Tetrad Graduate Program, University of California, San Francisco, CA 94158, USA., Chen X; Gladstone/UCSF Center for Cell Circuitry, Gladstone Institutes, San Francisco, CA 94158, USA., Martin B; Gladstone/UCSF Center for Cell Circuitry, Gladstone Institutes, San Francisco, CA 94158, USA.; Institute for Molecules and Materials, Radboud University, 6525 AJ Nijmegen, the Netherlands., Chaturvedi S; Gladstone/UCSF Center for Cell Circuitry, Gladstone Institutes, San Francisco, CA 94158, USA., Hwang DW; Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA., Li W; Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA., Yu C; Gladstone Institute of Cardiovascular Disease, Gladstone Institutes, San Francisco, CA 94158, USA., Ding S; Gladstone Institute of Cardiovascular Disease, Gladstone Institutes, San Francisco, CA 94158, USA.; School of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, China., Thomson M; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA., Singer RH; Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA., Coleman RA; Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA., Hansen MMK; Institute for Molecules and Materials, Radboud University, 6525 AJ Nijmegen, the Netherlands., Weinberger LS; Gladstone/UCSF Center for Cell Circuitry, Gladstone Institutes, San Francisco, CA 94158, USA. leor.weinberger@gladstone.ucsf.edu.; Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94158, USA.; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA.

Publikováno v: Science (New York, N.Y.) [Science] 2021 Aug 20; Vol. 373 (6557). Date of Electronic Publication: 2021 Jul 22.