Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Hans-Peter Knopf"'
Surface display vectors for selective detection and isolation of high level antibody producing cells
Autor:
Hans-Peter Knopf, Thomas Jostock, Sabine Lang, Johannes Wichter, Nommay Audrey Josiane, Delia Drewello, Burkhard Wilms
Publikováno v:
Biotechnology and Bioengineering. 113:2386-2393
Cell line generation for production of biopharmaceuticals in mammalian cells usually involves intensive screening of clones to identify the rare high producers. In order to facilitate efficient and selective fluorescence activated cell sorting (FACS)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bf13b9ad2f5bf360a45f597743d08ae3
https://doi.org/10.1002/bit.26000
https://doi.org/10.1002/bit.26000
Surface display vectors for selective detection and isolation of high level antibody producing cells
Autor:
Sabine, Lang, Delia, Drewello, Johannes, Wichter, Audrey, Nommay, Burkhard, Wilms, Hans-Peter, Knopf, Thomas, Jostock
Publikováno v:
Biotechnology and bioengineering. 113(11)
Cell line generation for production of biopharmaceuticals in mammalian cells usually involves intensive screening of clones to identify the rare high producers. In order to facilitate efficient and selective fluorescence activated cell sorting (FACS)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::732a28ca3a53addb69912018b840ae45
https://pubmed.ncbi.nlm.nih.gov/27144878
https://pubmed.ncbi.nlm.nih.gov/27144878
Autor:
Peter Schollmeyer, Hans-Peter Knopf, Kathrin Bauermeister, Gustav J. Dobos, Nadja Almanasreh, Meike Burger
Publikováno v:
Immunobiology. 200:62-76
Studies on human macrophages are restricted due to difficulties in isolating significant numbers of human macrophages. High numbers of monocytes/macrophages can be obtained from peritonitis effluents of patients treated with peritoneal dialysis. To d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27c4e4f4f31e72c3a4767ea68b1c5c6d
https://doi.org/10.1016/s0171-2985(99)80033-3
https://doi.org/10.1016/s0171-2985(99)80033-3
Autor:
R. Engelhardt, Ulrike Haus, Roland Mertelsmann, Hans-Peter Knopf, Alexander Seiz, Florian Otto, Astrid Tschiersch, Alexander Kiani, Lothar Färber, Peter Stütz, Elke Gaboriau, Chris Galanos
Publikováno v:
Blood. 90:1673-1683
Interfering with the endotoxin-mediated cytokine cascade is thought to be a promising approach to prevent septic complications in gram-negative infections. The synthetic lipid A analog SDZ MRL 953 has been shown to be protective against endotoxic sho
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74960d6a32ed9fd0005c4409adabad5d
https://doi.org/10.1182/blood.v90.4.1673
https://doi.org/10.1182/blood.v90.4.1673
Autor:
Chris Galanos, Andreas Sing, Hans Peter Knopf, Tetsuya Takakuwa, Rita Carsetti, Marina A. Freudenberg
Publikováno v:
European Journal of Immunology. 26:2686-2692
The involvement of CD14 in lipopolysaccharide (LPS) recognition and signaling has been demonstrated in several studies. For this reason, we investigated whether the resistance of Lpsd mice to LPS might be related to an impaired CD14 expression. We co
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::883a089632f8de7f9583a46239e5d3ff
https://doi.org/10.1002/eji.1830261121
https://doi.org/10.1002/eji.1830261121
Autor:
G J Dobos, Hans-Peter Knopf, Johannes Norgauer, Meike Sach, Alexander Kapp, Jörn Elsner, Peter Schollmeyer
Publikováno v:
Immunobiology. 193:456-464
During bacterial peritonitis of patients on continuous ambulatory peritoneal dialysis (CAPD) leukocytes, particularly polymorphonuclear neutrophilic granulocytes (PMNs), migrate into the peritoneal cavity. However, at the site of inflammation PMNs ar
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eec776605de0c1829c9250a5d9c1dda7
https://doi.org/10.1016/s0171-2985(11)80430-4
https://doi.org/10.1016/s0171-2985(11)80430-4
Autor:
Thomas, Jostock, Hans-Peter, Knopf
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 899
Many therapeutically relevant proteins, like IgG antibodies, are highly complex, multimeric glycoproteins that are difficult to express in microbial systems and thus usually produced in mammalian host cells. During the past two decades, stable mammal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::91d5831b05936649fc50ecddef88e505
https://pubmed.ncbi.nlm.nih.gov/22735957
https://pubmed.ncbi.nlm.nih.gov/22735957
Autor:
Thomas Jostock, Hans-Peter Knopf
Publikováno v:
Methods in Molecular Biology ISBN: 9781617799204
Many therapeutically relevant proteins, like IgG antibodies, are highly complex, multimeric glycoproteins that are difficult to express in microbial systems and thus usually produced in mammalian host cells. During the past two decades, stable mammal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::56a765cffe656eee1223d27d3708c1d8
https://doi.org/10.1007/978-1-61779-921-1_15
https://doi.org/10.1007/978-1-61779-921-1_15
Publikováno v:
Applied microbiology and biotechnology. 87(4)
The recently described 2A/furin technology combines both chains of the antibody in a single open reading frame. Upon translation and secretion, the peptide is processed by the cell to generate native fully functional IgG antibodies. Here, we describe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4bdc7287dc5155423fda26260754e4a5
https://pubmed.ncbi.nlm.nih.gov/20461511
https://pubmed.ncbi.nlm.nih.gov/20461511
Autor:
Harald Loppnow, Marina A. Freudenberg, Thomas Merlin, Chris Galanos, Peter J. Nielsen, Andreas Sing, Hans-Peter Knopf
Publikováno v:
Infection and immunity. 68(3)
We investigated the reason for the inability of lipopolysaccharide (LPS)-resistant (Lps-defective [Lpsd]) C57BL/10ScCr mice to produce beta interferon (IFN-β) when stimulated with bacteria. For this purpose, the IFN-β and other macrophage cytokine
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c7266b9cf56b1378b104a4ecb07097d
https://pubmed.ncbi.nlm.nih.gov/10678979
https://pubmed.ncbi.nlm.nih.gov/10678979