Finding Na,K-ATPase: I - From Cell to Molecule

Autor: Hans-Jürgen Apell

Publikováno v: Substantia, Vol 2, Iss 1 (2018)

The oppositely oriented concentration gradients of Na+ and K+ ions across the cell membrane as found in animal cells led to the requirement of an active ion-transport mechanism that maintains this steady-state condition. As solution of this problem t

Externí odkaz: https://doaj.org/article/298662d6c56a481d926d9b86c6d364f2

Partial Reactions of the Na,K-ATPase: Determination of Activation Energies and an Approach to Mechanism

Autor: Hans-Jürgen Apell, Milena Roudna

Publikováno v: The Journal of Membrane Biology

Kinetic experiments were performed with preparations of kidney Na,K-ATPase in isolated membrane fragments or reconstituted in vesicles to obtain information of the activation energies under turnover conditions and for selected partial reactions of th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a8962a70f7258f98e33900e4eb82528
https://pubmed.ncbi.nlm.nih.gov/33184678

Electrostatic Stabilization Plays a Central Role in Autoinhibitory Regulation of the Na+,K+-ATPase

Autor: Hans-Jürgen Apell, Minwoo Han, Qiucen Jiang, Alvaro Garcia, Ronald J. Clarke, Himanshu Khandelia, Flemming Cornelius

Publikováno v: Jiang, Q, Garcia, A, Han, M, Cornelius, F, Apell, H J, Khandelia, H & Clarke, R J 2017, ' Electrostatic Stabilization Plays a Central Role in Autoinhibitory Regulation of the Na +,K +-ATPase ', Biophysical Journal, vol. 112, no. 2, pp. 288-299 . https://doi.org/10.1016/j.bpj.2016.12.008
Jiang, Q, Garcia, A, Han, M, Cornelius, F, Apell, H-J, Khandelia, H & Clarke, R J 2017, ' Electrostatic Stabilization Plays a Central Role in Autoinhibitory Regulation of the Na+,K+-ATPase ', Biophysical Journal, vol. 112, no. 2, pp. 288-299 . https://doi.org/10.1016/j.bpj.2016.12.008

The Na+,K+-ATPase is present in the plasma membrane of all animal cells. It plays a crucial role in maintaining the Na+ and K+ electrochemical potential gradients across the membrane, which are essential in numerous physiological processes, e.g., ner

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ee9c8d156e4fa59b9da3015cb7f9e5c
https://doi.org/10.1016/j.bpj.2016.12.008

Binding of Potassium Ions Inside the Access Channel at the Cytoplasmic Side of Na+,K+-ATPase

Autor: V. Ye. Vishnyakova, Valerij S. Sokolov, V. Yu. Tashkin, A. O. Terentjev, Hans-Jürgen Apell

Binding of potassium ions through an access channel from the cytoplasmic side of Na+,K+-ATPase and the effect of pH and magnesium ions on this process have been studied. The studies were carried out by a previously developed method of measuring small

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::606eb5ac63068a87941423b12e09de92

Electrogenic binding of ions at the cytoplasmic side of the Na+,K+-ATPase

Autor: A. I. Ilovaisky, V. Yu. Tashkin, Hans-Jürgen Apell, Valerij S. Sokolov, A. N. Gavril’chik

Publikováno v: Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology. 9:92-99

Electrogenic binding of ions from the cytoplasmic side of the Na+,K+-ATPase has been studied by measurements of changes of the membrane capacitance and conductance triggered by a jump of pH or of the sodium-ion concentration in the absence of ATP. Th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b61553921053bdab100dac613949270b
https://doi.org/10.1134/s1990747815020105