Zobrazeno 1 - 10
of 46
pro vyhledávání: '"Hans Probst"'
Cdk2 activity is dispensable for triggering replicon initiation after transient hypoxia in T24 cells
Publikováno v:
FEBS Journal. 272:5623-5634
We examined whether the fast release of replicon initiation after sudden O2 recovery of hypoxically incubated mammalian cells depends on kinase activity of Cdk2. We used a system based on starved/refed T24 cells elaborated previously for such investi
Publikováno v:
Biochemical Journal. 386:557-566
SV40 (simian virus 40)-infected CV1 cells were permeabilized with Staphylococcus aureus α-toxin for small molecules (
Publikováno v:
European Journal of Biochemistry. 270:3880-3890
It was shown previously [Riedinger, H. J., van Betteraey-Nikoleit, M & Probst, H. (2002) Eur. J. Biochem.269, 2383-2393] that initiation of in vivo SV40 DNA replication is reversibly suppressed by hypoxia in a state where viral minichromosomes exhibi
Publikováno v:
European Journal of Biochemistry. 269:2383-2393
Hypoxia interrupts the initiation of simian virus 40 (SV40) replication in vivo at a stage situated before unwinding of the␣origin region. After re-oxygenation, unwinding followed by a synchronous round of viral replication takes place. To␣furthe
Autor:
Hans-Jörg Riedinger, Hans Probst, Maria van Betteraey-Nikoleit, Karl-Heinz Eisele, Uwe Hilfrich
Publikováno v:
Journal of Biological Chemistry. 276:47122-47130
Simian virus 40 (SV40)-infected CV1 cells exposed to hypoxia show an inhibition of viral replication. Reoxygenation after several hours of hypoxia results in new initiations followed by a nearly synchronous round of SV40 replication. In this communic
Publikováno v:
Molecular and Cellular Biology. 13:5020-5033
Twelve-hour exposure of G1 Ehrlich ascites cells to controlled hypoxia (200 ppm of O2 at 1 bar) suppressed replicon initiation. Synchronous cycling, beginning with a normal S phase, was released by reoxygenation immediately. The addition of cyclohexi
Publikováno v:
European journal of biochemistry. 270(19)
It was shown previously [Riedinger, H. J., van Betteraey-Nikoleit, MProbst, H. (2002) Eur. J. Biochem.269, 2383-2393] that initiation of in vivo SV40 DNA replication is reversibly suppressed by hypoxia in a state where viral minichromosomes exhibit a
Publikováno v:
Biological chemistry. 380(12)
In order to elucidate whether data about the fast regulation of DNA replication in dependence on oxygen supply and on a functioning protein synthesis, previously elaborated with Ehrlich ascites cells, are valid for human cells too, we repeated key ex
Simian virus 40 (SV40)-infected CV1 cells transiently exposed to hypoxia show a burst of viral replication immediately after reoxygenation. DNA precursor incorporation and analysis of growing daughter strands by alkaline sedimentation demonstrated th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad5db3c85f57d0ee4740d6aef8fcedc6
https://europepmc.org/articles/PMC104469/
https://europepmc.org/articles/PMC104469/
Publikováno v:
European journal of biochemistry. 244(2)
Cultured Ehrlich ascites cells were exposed to different oxygen tensions (ranging from nearly complete anoxia to 95% O2 at 10(5) Pa) and to transient (5-10 h) hypoxia (0.02% O2 at 10(5) Pa). Treated cells were examined with respect to the intracellul