Zobrazeno 1 - 10
of 107
pro vyhledávání: '"Hans J. Lipps"'
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 2, Iss C (2013)
The vector pEPI was the first nonviral and episomally replicating vector. Its functional element is an expression unit linked to a chromosomal scaffold/matrix attached region (S/MAR). The vector replicates autonomously with low copy number in various
Externí odkaz:
https://doaj.org/article/e3fb85bd56d1430699144ab77d4a21b7
Autor:
Matthias Möllenbeck, Yi Zhou, Andre R O Cavalcanti, Franziska Jönsson, Brian P Higgins, Wei-Jen Chang, Stefan Juranek, Thomas G Doak, Grzegorz Rozenberg, Hans J Lipps, Laura F Landweber
Publikováno v:
PLoS ONE, Vol 3, Iss 6, p e2330 (2008)
Programmed DNA elimination and reorganization frequently occur during cellular differentiation. Development of the somatic macronucleus in some ciliates presents an extreme case, involving excision of internal eliminated sequences (IESs) that interru
Externí odkaz:
https://doaj.org/article/2ddf985416264925b1e787188280ca88
Publikováno v:
Nucleic Acids Research
Autonomously replicating vectors represent a simple and versatile model system for genetic modifications, but their localization in the nucleus and effect on endogenous gene expression is largely unknown. Using circular chromosome conformation captur
Autor:
Hans J. Lipps, Jan Postberg
Publikováno v:
BIOspektrum. 23:14-17
For many years it is known that nucleic acids containing runs of guanines can fold into four-stranded structures, G-quadruplexes, but often they were regarded as structural curiosity. Only recently evidence accumulated that these structures occur in
Autor:
Philip Boehme, Hans J. Lipps, Maria Schnödt-Fuchs, Claudia Hagedorn, Heba Abdelrazik, Hildegard Büning
Publikováno v:
Human gene therapy. 28(12)
Adeno-associated virus (AAV) vectors are one of the most frequently applied gene transfer systems in research and human clinical trials. Since AAV vectors do not possess an integrase activity, application is restricted to terminally differentiated ti
Publikováno v:
Epigenomics
We introduce ciliated protozoa, and more specifically the stichotrichous ciliates Oxytricha and Stylonychia, as biological model systems for the analysis of programmed DNA-reorganization processes during nuclear differentiation. These include DNA exc
De novo addition of telomeric sequences can occur at broken chromosomes and must be well controlled, which is essential during programmed DNA reorganization processes. In ciliated protozoa an extreme form of DNA-reorganization is observed during macr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73cc60c492064718ebf62cc57c918456
https://europepmc.org/articles/PMC4993297/
https://europepmc.org/articles/PMC4993297/
Publikováno v:
Gene
It is well established that G-quadruplex DNA structures form at ciliate telomeres and their formation throughout the cell-cycle by telomere-end-binding proteins (TEBPs) has been analyzed. During replication telomeric G-quadruplex structure has to be
Autor:
Hans J. Lipps, Sina Rupprecht
Publikováno v:
Gene. 439:95-101
Non-viral episomal vectors are regarded as attractive alternatives to currently used virus-based vectors in gene therapy. In addition, they represent a minimal model system to study the epigenetic control of basic nuclear processes, such as transcrip
Publikováno v:
Nature Structural & Molecular Biology. 15:598-604
The telomeric G-overhangs of the ciliate Stylonychia lemnae fold into a G-quadruplex DNA structure in vivo. Telomeric G-quadruplex formation requires the presence of two telomere end binding proteins, TEBPalpha and TEBPbeta, and is regulated in a cel