Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Hans H. Liao"'
Publikováno v:
Applied and Environmental Microbiology. 72:6402-6404
The development of lysine 2,3-aminomutase as a robust biocatalyst hinges on the development of an in vivo activation system to trigger catalysis. This is the first report to show that, in the absence of chemical reductants, lysine 2,3-aminomutase act
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8611e8c75527bfc8bc774fe1fd0f8259
https://doi.org/10.1128/aem.01143-06
https://doi.org/10.1128/aem.01143-06
Autor:
Hazel M. Holden, Matthew M. Benning, Joshua Sakon, Ivan Rayment, Agnes M. Kanikula, Hans H. Liao
Publikováno v:
Biochemistry. 32:11977-11984
Kanamycin nucleotidyltransferase, as originally isolated from Staphylococcus aureus, inactivates the antibiotic kanamycin by catalyzing the transfer of a nucleotidyl group from nucleoside triphosphates such as ATP to the 4'-hydroxyl group of the amin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44f83d4336fd616c638b27080cbc48e0
https://doi.org/10.1021/bi00096a006
https://doi.org/10.1021/bi00096a006
Publikováno v:
Applied and Environmental Microbiology. 57:3541-3546
The dha regulon in Klebsiella pneumoniae enables the organism to grow anaerobically on glycerol and produce 1,3-propanediol (1,3-PD). Escherichia coli, which does not have a dha system, is unable to grow anaerobically on glycerol without an exogenous
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2b7dfb17824b490a13dae7fecaccfea
https://doi.org/10.1128/aem.57.12.3541-3546.1991
https://doi.org/10.1128/aem.57.12.3541-3546.1991
Autor:
Hans H. Liao, Agnes M. Kanikula
Publikováno v:
Current Microbiology. 21:301-306
Recombinant plasmids carrying either the wildtype kanamycin nucleotidyltransferase gene encoded originally by the mesophilic plasmid pUB110 or the gene encoding the thermostable TK101 mutant were constructed and introduced intoBacillus stearothermoph
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f6687629d4bc368c55dd445d17537c4b
https://doi.org/10.1007/bf02092095
https://doi.org/10.1007/bf02092095
Publikováno v:
Analytical chemistry. 77(20)
Stable isotope labeling of an intracellular chemical precursor or metabolite allows direct detection of downstream metabolites of that precursor, arising from novel enzymatic activity of interest, using metabolite profiling liquid chromatography-mass
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8b44f518d84b97ece437272dc249de28
https://pubmed.ncbi.nlm.nih.gov/16223264
https://pubmed.ncbi.nlm.nih.gov/16223264
Publikováno v:
Archives of biochemistry and biophysics. 295(1)
A thermostable mutant of kanamycin nucleotidyltransferase isolated by cloning and selection for kanamycin resistance in Bacillus stearothermophilus at 70 °C has been crystallized in a form suitable for high-resolution diffraction analysis. This enzy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a2805b3d481a9a0778e64df6ad8ef27
https://pubmed.ncbi.nlm.nih.gov/1315501
https://pubmed.ncbi.nlm.nih.gov/1315501
Autor:
Hans H. Liao
Publikováno v:
Protein expression and purification. 2(1)
The expression of kanamycin nucleotidyltransferase (KNTase) in Escherichia coli results in different forms of the protein, depending on the temperature; soluble active enzyme is synthesized at 23 degrees C but the protein is mostly aggregated and ina
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6da24207065a9a0ebe413d2a03e5e31
https://pubmed.ncbi.nlm.nih.gov/1668268
https://pubmed.ncbi.nlm.nih.gov/1668268
Autor:
Jesse C. Rabinowitz, Hans H. Liao
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 608:301-314
An assay for Clostridium pasteurianum apoferredoxin messenger ribonucleic acid (mRNA) was developed, based on the synthesis of the protein in vitro. Quantitation of apoferredoxin synthesis was accomplished by trypsinization of the cell-free incubatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1aa2cc9047da8ab23ab476b525c181b3
https://doi.org/10.1016/0005-2787(80)90176-8
https://doi.org/10.1016/0005-2787(80)90176-8
Autor:
Wong DWS; Western Regional Research Center, USDA-ARS, Albany, CA, USA. Dominic.Wong@ars.usda.gov., Chan VJ; Western Regional Research Center, USDA-ARS, Albany, CA, USA., Liao H; Cargill Biotechnology Development Center, Minneapolis, MN, USA.
Publikováno v:
Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2019 Oct; Vol. 103 (20), pp. 8449-8457. Date of Electronic Publication: 2019 Sep 12.
Autor:
Shang M; Western Regional Research Center, USDA-ARS, Albany, CA, USA., Chan VJ; Western Regional Research Center, USDA-ARS, Albany, CA, USA., Wong DWS; Western Regional Research Center, USDA-ARS, Albany, CA, USA., Liao H; Cargill Biotechnology Development Center, Minneapolis, MN, USA.
Publikováno v:
MethodsX [MethodsX] 2018 Jun 20; Vol. 5, pp. 669-675. Date of Electronic Publication: 2018 Jun 20 (Print Publication: 2018).