Descriptive parameters for revealing substitution patterns of sugar beet pectins using pectolytic enzymes

Autor: Hans Christian Buchholt, Henk A. Schols, C.A. Remoroza, Harry Gruppen

Publikováno v: Carbohydrate Polymers, 101, 1205-1215
Carbohydrate Polymers 101 (2014)

Enzymatic fingerprinting was applied to sugar beet pectins (SBPs) modified by either plant or fungal pectin methyl esterases and alkali catalyzed de-esterification to reveal the ester distributions over the pectin backbone. A simultaneous pectin lyas

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34bed482a24d7c8f1e00a070f0d32a4f
https://doi.org/10.1016/j.carbpol.2013.10.034

Polyelectrolyte behaviour and calcium binding properties of sugar beet pectins differing in their degrees of methylation and acetylation

Autor: Marie-Christine Ralet, Marie-Jeanne Crépeau, Jean-François Thibault, Hans-Christian Buchholt

Publikováno v: Biochemical Engineering Journal. 16:191-201

Five series of pectins with different levels and patterns of methyl esterification and/or different levels of acetyl esterification were produced by chemical or enzymatic treatment of an acid-extracted sugar beet pectin. The relationship between pKa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c2adb613e10e61d8ec63c5227f5569fb
https://doi.org/10.1016/s1369-703x(03)00037-8

Analysis of pectic epitopes recognised by hybridoma and phage display monoclonal antibodies using defined oligosaccharides, polysaccharides, and enzymatic degradation

Autor: Alphons G. J. Voragen, J. Paul Knox, Gerrit Limberg, Hans Christian Buchholt, Gert-Jan W. M. van Alebeek, Jaap Visser, Tove M.I.E. Christensen, Jacques A.E. Benen, Jørn Dalgaard Mikkelsen, William G.T. Willats

Publikováno v: Carbohydrate Research : an international journal 327 (2000)
Carbohydrate Research : an international journal, 327, 309-320

The structure of epitopes recognised by anti-pectin monoclonal antibodies (mAbs) has been investigated using a series of model lime-pectin samples with defined degrees and patterns of methyl esterification, a range of defined oligogalacturonides and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::644abf73de3aafd9be5a15319239c878
https://doi.org/10.1016/s0008-6215(00)00039-2

Modulation of the degree and pattern of methyl-esterification of pectic homogalacturonan in plant cell walls

Autor: J. Paul Knox, Caroline Orfila, Susan E. Marcus, Gert-Jan W. M. van Alebeek, Hans Christian Buchholt, Jørn Dalgaard Mikkelsen, G Limberg, William G.T. Willats, Tove M.I.E. Christensen, Brent S. Murray, Alphons G. J. Voragen

Publikováno v: Journal of Biological Chemistry 276 (2001)
Journal of Biological Chemistry, 276, 19404-19413

From the Centre for Plant Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom, § Danisco Biotechnology, Langebrogade 1, DK 1001 Copenhagen K, Denmark, Danisco Cultor, Edwin Rahrs Vej 38, DK-8220 Brabrand, Denmark, the ** Department of Agrot

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13e23a46fb3fd7bf675f6145c594dc3b
https://research.wur.nl/en/publications/modulation-of-the-degree-and-pattern-of-methyl-esterification-of-

Quantification of the amount of galacturonic acid residues in blocksequences in pectin homogalacturonan by enzymatic fingerprinting with exo- and endo-polygalacturonase II from Aspergillus niger

Autor: Roman Körner, Gerrit Limberg, Tove M.I.E. Christensen, Jørn Dalgaard Mikkelsen, Peter Roepstorff, Hans Christian Buchholt

Publikováno v: Limberg, G, Körner, R, Buchholt, H C, Christensen, T M, Roepstorff, P & Mikkelsen, J D 2000, ' Quantification of the amount of galacturonic acid residues in blocksequences in pectin homogalacturonan by enzymatic fingerprinting with exo-and endo-polygalacturonase II from Aspergillus niger. ', Carbohydrate Research, vol. 327, no. 3, pp. 321-32 . https://doi.org/10.1016/S0008-6215(00)00068-9

Udgivelsesdato: 2000-Jul-24 A method to determine the amount of galacturonic acid in blocksequence (BS) in pectin homogalacturonan (HG) is described. The method is based on a combination of endopolygalacturonase II (endo-PG II) and exopolygalacturona

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3536a74cb786065ff8ed9e44b5f0b653
https://pubmed.ncbi.nlm.nih.gov/10945680