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Akademický článek

Structural and functional analysis of the promiscuous AcrB and AdeB efflux pumps suggests different drug binding mechanisms

Autor: Alina Ornik-Cha, Julia Wilhelm, Jessica Kobylka, Hanno Sjuts, Attilio V. Vargiu, Giuliano Malloci, Julian Reitz, Anja Seybert, Achilleas S. Frangakis, Klaas M. Pos

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)

Resistance-nodulation-cell division (RND)-type tripartite efflux pumps confer multidrug resistance to Gram-negative bacteria. Here, structural and functional analyses of AdeB from Acinetobacter baumannii and AcrB from Escherichia coli provide insight

Externí odkaz: https://doaj.org/article/0fa39d64c8884cc2b68766a6f47a6795

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Direct Comparison of Label-Free Biosensor Binding Kinetics Obtained on the Biacore 8K and the Carterra LSA

Autor: Hanno Sjuts, Yasmina Noubia Abdiche, Jennifer Clark, Stuart Knowling

Publikováno v: SLAS Discovery. 25:977-984

Funding pressure on the pharmaceutical industry to deliver new medicines to the market under aggressive timelines has led to a demand for analytical tools with higher detection sensitivity, increased throughput, and automation to speed up research an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06b7678c42a1d59a0bba79958215aac9
https://doi.org/10.1177/2472555220934814

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Engineered Technologies and Bioanalysis of multispecific Antibody Formats

Autor: Ercole Rao, Melanie Fischer, Marta Amaral, Sandra Weil, Soraya Hölper, Hanno Sjuts, Christian Lange, Jennifer Jung, Katarina Radoevic

Publikováno v: Journal of Applied Bioanalysis, Vol 6, Iss 1, Pp 26-51 (2020)

The idea of designing multispecific antibodies capable of simultaneously engaging two or more epitopes on the same or different antigens was developed more than 50 years ago. However, the molecular complexity of such molecules may pose significant ch

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d8b04b9423433560b509d38e793e59c
https://doi.org/10.17145/jab.20.005

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A multivalent antibody assembled from different building blocks using tag/catcher systems: a case study

Autor: Christof Schindler, Christine Faust, Hanno Sjuts, Christian Lange, Jennifer Kühn, Werner Dittrich, Wulf Dirk Leuschner, Werner Schiebler, Joachim Hofmann, Ercole Rao, Thomas Langer

Publikováno v: Protein Engineering, Design and Selection. 35

The field of therapeutic antibodies and, especially bi- or multispecific antibodies, is growing rapidly. Especially for treating cancers, multispecific antibodies are very promising, as there are multiple pathways involved and multispecific antibodie

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::795f90423eb72ef734dd79403b0a33c6
https://doi.org/10.1093/protein/gzac014

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Matching pH values for antibody stabilization and crystallization suggest rationale for accelerated development of biotherapeutic drugs

Autor: Christian K Engel, Yatin Gokarn, Till Bussemer, Hanno Sjuts, Herman Schreuder

Publikováno v: Drug Development Research. 81:329-337

Monoclonal antibodies (mAbs) are currently leading products in the global biopharmaceutical market. Multiple mAbs are in clinical development and novel biotherapeutic protein scaffolds, based on the canonical immunoglobulin G (IgG) fold, are emerging

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e9e3b52ba347cd9f1b20fc56638d487b
https://doi.org/10.1002/ddr.21624

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Approved Drugs Containing Thiols as Inhibitors of Metallo-β-lactamases: Strategy To Combat Multidrug-Resistant Bacteria

Autor: Müller Hf, Stephan Göttig, Ewgenij Proschak, Cuesta-Bernal J, Denia Frank, Arno Koenigs, Klaas M. Pos, Franca-Maria Klingler, Hanno Sjuts, Jasmin El-Delik, Thomas A. Wichelhaus, Denys Pogoryelov

Publikováno v: Journal of Medicinal Chemistry. 58:3626-3630

Resistance to β-lactam antibiotics can be mediated by metallo-β-lactamase enzymes (MBLs). An MBL inhibitor could restore the effectiveness of β-lactams. We report on the evaluation of approved thiol-containing drugs as inhibitors of NDM-1, VIM-1,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c1e1cd8f6aff3d6af7b0a63cc54e772d
https://doi.org/10.1021/jm501844d

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Molecular basis for inhibition of AcrB multidrug efflux pump by novel and powerful pyranopyridine derivatives

Autor: Steven M. Kwasny, Timothy J. Opperman, Xiaoyuan Ding, Hanno Sjuts, Terry L. Bowlin, Alina R. Ornik, Attilio Vittorio Vargiu, Son T. Nguyen, Paolo Ruggerone, Hiroshi Nikaido, Hong-Suk Kim, Klaas M. Pos

Publikováno v: Sjuts, H; Vargiu, AV; Kwasny, SM; Nguyen, ST; Kim, HS; Ding, X; et al.(2016). Molecular basis for inhibition of AcrB multidrug efflux pump by novel and powerful pyranopyridine derivatives. Proceedings of the National Academy of Sciences of the United States of America, 113(13), 3509-3514. doi: 10.1073/pnas.1602472113. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/36v4w9g5
Proceedings of the National Academy of Sciences of the United States of America, vol 113, iss 13
Proceedings of the National Academy of Sciences of the United States of America 113(13), 3509-3514 (2016). doi:10.1073/pnas.1602472113

Proceedings of the National Academy of Sciences of the United States of America 113(13), 3509 - 3514(2016). doi:10.1073/pnas.1602472113
The Escherichia coli AcrAB-TolC efflux pump is the archetype of the resistance nodulation cell division (RND)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e985e03830e4a69fcfc3e2102d8c7b56
https://pubmed.ncbi.nlm.nih.gov/26976576

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Heterologous expression, purification and cofactor reconstitution of the reductive dehalogenase PceA from Dehalobacter restrictus

Autor: Stephen E. J. Rigby, Karl Fisher, Mark S. Dunstan, David Leys, Hanno Sjuts

Publikováno v: Protein Expression and Purification

Organohalide respiration is used by a limited set of anaerobic bacteria to derive energy from the reduction of halogenated organic compounds. The enzymes that catalyze the reductive dehalogenation reaction, the reductive dehalogenases, represent a no

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8320bc0b396b2b0c390942cc8cde61c0
https://doi.org/10.1016/j.pep.2012.08.007

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Epoxyqueuosine Reductase Structure Suggests a Mechanism for Cobalamin-dependent tRNA Modification

Autor: Karl Fisher, Stephen E. J. Rigby, Linus O. Johannissen, Mark S. Dunstan, David Leys, Sam Hay, Karl A. P. Payne, Hanno Sjuts, Perdita E. Barran, Bruno Bellina

Publikováno v: The Journal of Biological Chemistry

Background: Little is known about epoxyqueuosine reductase (QueG), which catalyzes the final step in the biosynthesis of queuosine. Results: We report solution and structural characterization of Streptococcus thermophilus QueG. Conclusion: The QueG s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c57e4b6cfa7d6f33e7ec9cae4505ecf
https://pubmed.ncbi.nlm.nih.gov/26378237

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Structures of the methyltransferase component of Desulfitobacterium hafniense DCB-2 O-demethylase shed light on methyltetrahydrofolate formation

Autor: Hanno Sjuts, David Leys, Karl Fisher, Mark S. Dunstan

Publikováno v: Acta crystallographica. Section D, Biological crystallography. 71(Pt 9)

O-Demethylation by acetogenic or organohalide-respiring bacteria leads to the formation of methyltetrahydrofolate from aromatic methyl ethers.O-Demethylases, which are cobalamin-dependent, three-component enzyme systems, catalyse methyl-group transfe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4da52fb1d9139a03c199c701c326e6b0
https://pubmed.ncbi.nlm.nih.gov/26327380

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