Enzymatic incorporation of an isotope-labeled adenine into RNA for the study of conformational dynamics by NMR.

Autor: Hannes Feyrer, Cenk Onur Gurdap, Maja Marušič, Judith Schlagnitweit, Katja Petzold

Publikováno v: PLoS ONE, Vol 17, Iss 7, p e0264662 (2022)

Solution NMR spectroscopy is a well-established tool with unique advantages for structural studies of RNA molecules. However, for large RNA sequences, the NMR resonances often overlap severely. A reliable way to perform resonance assignment and allow

Externí odkaz: https://doaj.org/article/cf7747cb2acf4cc9b950f35640c8501f

Mass spectrometry of RNA-binding proteins during liquid-liquid phase separation reveals distinct assembly mechanisms and droplet architectures

Autor: Cagla Sahin, Aikaterini Motso, Xinyu Gu, Hannes Feyrer, Dilraj Lama, Tina Arndt, Anna Rising, Genis Valentin Gese, B. Martin Hällberg, Erik. G. Marklund, Nicholas P. Schafer, Katja Petzold, Kaare Teilum, Peter G. Wolynes, Michael Landreh

Publikováno v: Sahin, C, Motso, A, Gu, X, Feyrer, H, Lama, D, Arndt, T, Rising, A, Gese, G V, Haellberg, B M, Marklund, E G, Schafer, N P, Petzold, K, Teilum, K, Wolynes, P G & Landreh, M 2023, ' Mass Spectrometry of RNA-Binding Proteins during Liquid-Liquid Phase Separation Reveals Distinct Assembly Mechanisms and Droplet Architectures ', Journal of the American Chemical Society, vol. 145, no. 19 . https://doi.org/10.1021/jacs.3c00932

Liquid–liquid phase separation (LLPS) of heterogeneous ribonucleoproteins (hnRNPs) drives the formation of membraneless organelles, but structural information about their assembled states is still lacking. Here, we address this challenge through a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93e59656385341f0238781c895fc6b2c
https://doi.org/10.1101/2022.09.28.509878

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA

Autor: Judith Schlagnitweit, Katja Petzold, Hannes Feyrer

Publikováno v: Journal of Visualized Experiments.

RNA is a highly flexible biomolecule, wherein changes in structures play crucial roles in the functions that RNA molecules execute as cellular messengers and modulators. While these dynamic states remain hidden to most structural methods, R1ρ relaxa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3af54ddd4dee3c344237c0bfc7a22ff8
https://doi.org/10.3791/62470

Modulating Hinge Flexibility in the APP Transmembrane Domain Alters γ-Secretase Cleavage

Autor: Dieter Langosch, Hannes Heinel, Christina Scharnagl, Claudia Muhle-Goll, Harald Steiner, Philipp Högel, Simon Menig, Daniel Huster, Alexander Götz, Nadine Mylonas, Frits Kamp, Burkhard Luy, Alexander Vogel, Mara Silber, Hannes Feyrer

Publikováno v: Biophys J
Biophysical journal 116(11), 2103-2120 (2019). doi:10.1016/j.bpj.2019.04.030

Intramembrane cleavage of the β-amyloid precursor protein C99 substrate by γ-secretase is implicated in Alzheimer’s disease pathogenesis. Biophysical data have suggested that the N-terminal part of the C99 transmembrane domain (TMD) is separated

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43179bc7d9a088e925fbc9d25ec19287
https://doi.org/10.1016/j.bpj.2019.04.030

Broadband RF-amplitude-dependent flip angle pulses with linear phase slope

Autor: Martin R M, Koos, Hannes, Feyrer, Burkhard, Luy

Publikováno v: Magnetic resonance in chemistry : MRC. 55(9)

Pulse sequences in NMR spectroscopy sometimes require the application of pulses with effective flip angles different from 90° and 180°. Previously (Magn. Reson. Chem. 2015, 53, 886-893), offset-compensated broadband excitation pulses with RF-amplit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::ee143990b7e5f4e8bf5c70e760864b7f
https://pubmed.ncbi.nlm.nih.gov/28321918