Modulating mutational outcomes and improving precise gene editing at CRISPR-Cas9-induced breaks by chemical inhibition of end-joining pathways

Autor: Joost Schimmel, Núria Muñoz-Subirana, Hanneke Kool, Robin van Schendel, Sven van der Vlies, Juliette A. Kamp, Femke de Vrij, Steven A. Kushner, Graeme C.M. Smith, Simon J. Boulton, Marcel Tijsterman

Publikováno v: Cell Reports, Vol 42, Iss 2, Pp 112019- (2023)

Summary: Gene editing through repair of CRISPR-Cas9-induced chromosomal breaks offers a means to correct a wide range of genetic defects. Directing repair to produce desirable outcomes by modulating DNA repair pathways holds considerable promise to i

Externí odkaz: https://doaj.org/article/2cda6fc70a1649d382e75e95931d82e5

Divergent Molecular and Cellular Responses to Low and High-Dose Ionizing Radiation

Autor: Bharath Sampadi, Sylvia Vermeulen, Branislav Mišovic, Jan J. Boei, Tanveer S. Batth, Jer-Gung Chang, Michelle T. Paulsen, Brian Magnuson, Joost Schimmel, Hanneke Kool, Cyriel S. Olie, Bart Everts, Alfred C. O. Vertegaal, Jesper V. Olsen, Mats Ljungman, Penny A. Jeggo, Leon H. F. Mullenders, Harry Vrieling

Publikováno v: Cells, Vol 11, Iss 23, p 3794 (2022)

Cancer risk after ionizing radiation (IR) is assumed to be linear with the dose; however, for low doses, definite evidence is lacking. Here, using temporal multi-omic systems analyses after a low (LD; 0.1 Gy) or a high (HD; 1 Gy) dose of X-rays, we s

Externí odkaz: https://doaj.org/article/c1d30103c56243f683bc88e1eec647e6

Small tandem dna duplications result from cst-guided pol α-primase action at dna break termini

Autor: Hanneke Kool, Robin van Schendel, Joost Schimmel, Núria Muñoz-Subirana, Marcel Tijsterman

Publikováno v: Nature Communications, 12(1)
Nature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
Nature Communications
Nature Communications, 12(1). NATURE PORTFOLIO

Small tandem duplications of DNA occur frequently in the human genome and are implicated in the aetiology of certain human cancers. Recent studies have suggested that DNA double-strand breaks are causal to this mutational class, but the underlying me

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5c526d0b0b6a552c5ea9e9c1f8197cd
https://hdl.handle.net/1887/3250283

A Ubiquitin-Binding Domain in Cockayne Syndrome B Required for Transcription-Coupled Nucleotide Excision Repair

Autor: Roy Anindya, Pierre-Olivier Mari, Wim Vermeulen, Giuseppina Giglia-Mari, Maria Fousteri, Jean-Marc Egly, Jesper Q. Svejstrup, Leon H.F. Mullenders, Ulrik Kristensen, Hanneke Kool

Publikováno v: Molecular Cell
Molecular Cell, Elsevier, 2010, 38 (5), pp.637-648. ⟨10.1016/j.molcel.2010.04.017⟩
Molecular Cell, 38(5), 637-648. Cell Press
Molecular Cell, 38(5), 637-648

Transcription-coupled nucleotide excision repair (TC-NER) allows RNA polymerase II (RNAPII)-blocking lesions to be rapidly removed from the transcribed strand of active genes. Defective TCR in humans is associated with Cockayne syndrome (CS), typical

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c512392d061239720a5fe948583e1cc
https://doi.org/10.1016/j.molcel.2010.04.017

Loss of heterozygosity in somatic cells of the mouse

Autor: Susan W.P. Wijnhoven, Corrie M.M. van Teijlingen, Albert A. van Zeeland, Harry Vrieling, Hanneke Kool

Publikováno v: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 473:23-36

Loss of heterozygosity (LOH) of tumour suppressor genes is a crucial step in the development of sporadic and hereditary cancer. Recently, we and others have developed mouse models in which the frequency and nature of LOH events at an autosomal locus

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8cd12ae336cc6cef173a05b55c6d7acb
https://doi.org/10.1016/s0027-5107(00)00163-9