Treatment of milk fat with sn-2 specific Pseudozyma antarctica lipase A for targeted hydrolysis of saturated medium and long-chain fatty acids

Autor: Kristiina Kruus, Susanna Rokka, Raija Lantto, Eila Järvenpää, Antti Nyyssölä, Hanna Kontkanen, Riitta Partanen, Martina Lille, Hanna Miettinen

Publikováno v: Nyyssölä, A, Miettinen, H, Kontkanen, H, Lille, M, Partanen, R, Rokka, S, Järvenpää, E, Lantto, R & Kruus, K 2015, ' Treatment of milk fat with sn-2 specific Pseudozyma antarctica lipase A for targeted hydrolysis of saturated medium and long-chain fatty acids ', International Dairy Journal, vol. 41, pp. 16-22 . https://doi.org/10.1016/j.idairyj.2014.09.003

Milk fat was treated with the sn- 2 specific PAN-2 lipase from Pseudozyma antarctica with the aim of achieving selective removal of saturated fatty acids with chain lengths between C12 and C16, which are abundant at the sn- 2 position. Hydrolysis was

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c3329e6309036697a6c5c654182118c2
https://doi.org/10.1016/j.idairyj.2014.09.003

Novel Coprinopsis cinerea Polyesterase that hydrolyzes cutin and Suberin

Autor: Johanna Buchert, Marjaana Rättö, Ann Westerholm-Parvinen, Ismo Mattila, Tiina Nakari-Setälä, Marzia Mohsina, Michael Bailey, Markku Saloheimo, Hanna Kontkanen, Nisse Kalkkinen

Publikováno v: Kontkanen, H, Westerholm-Parvinen, A, Saloheimo, M, Bailey, M, Rättö, M, Mattila, I, Mohsina, M, Kalkkinen, N, Nakari-Setälä, T & Buchert, J 2009, ' Novel Coprinopsis cinerea Polyesterase that hydrolyzes cutin and Suberin ', Applied and Environmental Microbiology, vol. 75, no. 7, pp. 2148-2157 . https://doi.org/10.1128/AEM.02103-08

Three cutinase gene-like genes from the basidiomycete Coprinopsis cinerea ( Coprinus cinereus ) found with a similarity search were cloned and expressed in Trichoderma reesei under the control of an inducible cbh1 promoter. The selected transformants

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1559b893dbf17617e706336e6f418c8a
https://cris.vtt.fi/en/publications/66f979b3-c2a9-4b7b-bcbc-0ec88ab42495

Characterisation of steryl esterase activities in commercial lipase preparations

Autor: Maija Tenkanen, Tapani Reinikainen, Richard Fagerström, Hanna Kontkanen

Publikováno v: Kontkanen, H, Tenkanen, M, Fagerström, R & Reinikainen, T 2004, ' Characterisation of steryl esterase activities in commercial lipase preparations ', Journal of Biotechnology, vol. 108, no. 1, pp. 51-59 . https://doi.org/10.1016/j.jbiotec.2003.11.003

Triglycerides, steryl esters, resin acids, free fatty acids and sterols are lipophilic extractives of wood (commonly referred to as pitch or wood resin) and have a negative impact on paper machine runnability and quality of paper. Thus, enzymes capab

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c97c650bcad61ca1e2787b478d4d27d
https://doi.org/10.1016/j.jbiotec.2003.11.003

A Cutinase from Trichoderma reesei with a Lid-Covered Active Site and Kinetic Properties of True Lipases

Autor: Antti Nyyssölä, Alain Roussel, Ann Westerholm-Parvinen, Stéphanie Blangy, Frédéric Carrière, Sawsan Amara, Eduardo Mateos-Diaz, Hanna Kontkanen, Christian Cambillau

Publikováno v: Journal of Molecular Biology
Journal of Molecular Biology, Elsevier, 2014, 426 (22), pp.3757-3772. ⟨10.1016/j.jmb.2014.09.003⟩
Journal of Molecular Biology, 2014, 426 (22), pp.3757-3772. ⟨10.1016/j.jmb.2014.09.003⟩
Roussel, A, Amara, S, Nyyssölä, A, Mateos-Diaz, E, Blangy, S, Kontkanen, H, Westerholm-Parvinen, A, Carriere, F & Cambillau, C 2014, ' A Cutinase from Trichoderma reesei with a lid-covered active site and kinetic properties of true lipases ', Journal of Molecular Biology, vol. 426, no. 22, pp. 3757-3772 . https://doi.org/10.1016/j.jmb.2014.09.003

Cutinases belong to the α/β-hydrolase fold family of enzymes and degrade cutin and various esters, including triglycerides, phospholipids and galactolipids. Cutinases are able to degrade aggregated and soluble substrates because, in contrast with t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a46415fa2a5a6fe0393abf8913c73350
https://hal.archives-ouvertes.fr/hal-02066305

Screening of microbes for novel acidic cutinases and cloning and expression of an acidic cutinase from Aspergillus niger CBS 513.88

Autor: Hanna Kontkanen, Saara Mikander, Heikki Kallio, Johanna Buchert, Ville Pihlajaniemi, Antti Nyyssölä, Kristiina Kruus, Riikka Järvinen

Publikováno v: Nyyssölä, A, Pihlajaniemi, V, Järvinen, R, Mikander, S, Kontkanen, H, Kruus, K, Kallio, H & Buchert, J 2013, ' Screening of microbes for novel acidic cutinases and cloning and expression of an acidic cutinase from Aspergillus niger CBS 513.88 ', Enzyme and Microbial Technology, vol. 52, no. 4-5, pp. 272-278 . https://doi.org/10.1016/j.enzmictec.2013.01.005

Isolates from gardening waste compost and 38 culture collection microbes were grown on agar plates at pH 4.0 with the cutinase model substrate polycaprolactone as a carbon source. The strains showing polycaprolactone hydrolysis were cultivated in liq

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b2999b609eebe34ae71fe4a08263f41
https://cris.vtt.fi/en/publications/a31890f3-a827-46d2-a481-9dfa23b84d85