Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Hankum Park"'
Autor:
Hankum Park, Frances V. Hundley, Qing Yu, Katherine A. Overmyer, Dain R. Brademan, Lia Serrano, Joao A. Paulo, Julia C. Paoli, Sharan Swarup, Joshua J. Coon, Steven P. Gygi, J. Wade Harper
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-21 (2022)
Methods to assess organellar content are important. Here, Park et al develop a method for rapid isolation of early/sorting endosomes and demonstrate the application of the approach for analysis of endosomal proteomes and lipidomes, and for analysis o
Externí odkaz:
https://doaj.org/article/1a034683ce2a4dcfbf3aa019ef9440d8
Autor:
Junhyeong Yim, Jaeseok Lee, Sihyeong Yi, Ja Young Koo, Sangmi Oh, Hankum Park, Seong Soon Kim, Myung Ae Bae, Jongmin Park, Seung Bum Park
Publikováno v:
Experimentalmolecular medicine.
Neuroinflammation is one of the critical processes implicated in central nervous system (CNS) diseases. Therefore, alleviating neuroinflammation has been highlighted as a therapeutic strategy for treating CNS disorders. However, the complexity of neu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d6df4498808420a9bf889762ade9189
https://pubmed.ncbi.nlm.nih.gov/36509830
https://pubmed.ncbi.nlm.nih.gov/36509830
Autor:
Hankum Park, Qing Yu, Frances V. Hundley, Katherine Overmyer, Lia Serrano, Joao A. Paulo, Julia C. Paoli, Sharan Swarup, Joshua J. Coon, Steven P. Gygi, J. Wade Harper
SUMMARYDegradation and recycling of plasma membrane-localized proteins occurs via the endolysosomal system, wherein endosomes bud into the cytosol from the plasma membrane and subsequently mature into degradative lysosomal compartments. The endolysos
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::27b0d598e10e281ea7343b61d6c65dea
https://doi.org/10.1101/2022.03.31.486371
https://doi.org/10.1101/2022.03.31.486371
Here we present a general protocol for immunological detection by Western blotting of APP and proteins of the endolysosomal system, including EEA1, RAB5, PSEN1, LAMP1, LAMP2, TMEM192, and BACE1.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7f2d3e208023f7af5edf1a106daed711
https://doi.org/10.17504/protocols.io.b6rurd6w
https://doi.org/10.17504/protocols.io.b6rurd6w
Lyso-IP is a method that allows for the isolation of lysosomes for proteomics and metabolomics (dx.doi.org/10.17504/protocols.io.bybjpskn; dx.doi.org/10.17504/protocols.io.bx9hpr36). We have developed an analogous approach for purification of early/s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8865623ec35aedf3bfa49d15306395ee
https://doi.org/10.17504/protocols.io.b6rjrd4n
https://doi.org/10.17504/protocols.io.b6rjrd4n
Previous studies have developed methods for isolation of lysosomes, mitochondria, and peroxisomes from non-denaturing extracts. Here we describe an approach for purification of early/sorting endosomes, providing a means by which to examine early aspe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0cdc7e6cf49ed2f0b828bfdfbb4470c5
https://doi.org/10.17504/protocols.io.b6rwrd7e
https://doi.org/10.17504/protocols.io.b6rwrd7e
Selective purification of early endosomes can be achieved through affinity capture of the early endosome-associated protein EEA1 (termed Endo-IP) (Park et al. 2022). These purified endosomes can be used for proteomic and lipidomic studies to obtain s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::36b637f192df1e2381588d3a8e4f12ff
https://doi.org/10.17504/protocols.io.b6rsrd6e
https://doi.org/10.17504/protocols.io.b6rsrd6e
Autor:
Jongmin Park, Heebum Song, Ala Jo, Tae Sung Kim, Seung Bum Park, Sang-Ho Choi, Myun Soo Kim, Hankum Park, Seung Min Kim
Publikováno v:
ACS Infectious Diseases. 6:3076-3082
An antivirulence agent against Vibrio vulnificus named quoromycin (QM) was discovered by a phenotype-based elastase inhibitor screening. Using the fluorescence difference in two-dimensional gel electrophoresis (FITGE) approach, SmcR, a quorum-sensing
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::55734b362a698b9bd1101a3b174f6369
https://doi.org/10.1021/acsinfecdis.0c00587
https://doi.org/10.1021/acsinfecdis.0c00587
Publikováno v:
Current Opinion in Chemical Biology. 50:66-72
Phenotypic screening has demonstrated its advantage in the discovery of first-in-class therapeutics, whereas target-based screening has showed strength for follower drugs. Owing to the unbiased nature of phenotypic screening, novel druggable proteins
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9dc1c49b44dccfde32e933fb954d5fa2
https://doi.org/10.1016/j.cbpa.2019.02.006
https://doi.org/10.1016/j.cbpa.2019.02.006
Autor:
Seung Bum Park, Hankum Park
Publikováno v:
Chemical Science
Target proteins and mechanism of a HeLa-specific cytotoxic compound were investigated by two thermal shift-based label-free proteomics methods.
Phenotypic screening can not only identify promising first-in-class drug candidates, but can also rev
Phenotypic screening can not only identify promising first-in-class drug candidates, but can also rev
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a655d393f29e6d6c6e31a433fbd01aa1
http://europepmc.org/articles/PMC6438152
http://europepmc.org/articles/PMC6438152