Deconvolution of synthetic mRNA expression: Nucleoside chemistry alters translatability

Autor: Hanieh Moradian, Marko Schwestka, Toralf Roch, Manfred Gossen

Publikováno v: Bioengineering & Translational Medicine, Vol 9, Iss 4, Pp n/a-n/a (2024)

Abstract Recent technological advances in the production of in vitro transcribed messenger RNA (IVT‐mRNA) facilitate its clinical use as well as its application in basic research. In this regard, numerous chemical modifications, which are not natur

Externí odkaz: https://doaj.org/article/a0f075f2894a4596b98a60d9d9662fbb

Cationic LNP-formulated mRNA expressing Tie2-agonist in the lung endothelium prevents pulmonary vascular leakage

Autor: Katrin Radloff, Birgitt Gutbier, Charlotte Maeve Dunne, Hanieh Moradian, Marko Schwestka, Manfred Gossen, Katharina Ahrens, Laura Kneller, Yadong Wang, Akanksha Moga, Leonidas Gkionis, Oliver Keil, Volker Fehring, Daniel Tondera, Klaus Giese, Ansgar Santel, Jörg Kaufmann, Martin Witzenrath

Publikováno v: Molecular Therapy: Nucleic Acids, Vol 34, Iss , Pp 102068- (2023)

Dysfunction of endothelial cells (ECs) lining the inner surface of blood vessels are causative for a number of diseases. Hence, the ability to therapeutically modulate gene expression within ECs is of high therapeutic value in treating diseases such

Externí odkaz: https://doaj.org/article/5eca547cc46b4d08ba4476f7d8468a32

Chemical modification of uridine modulates mRNA-mediated proinflammatory and antiviral response in primary human macrophages

Autor: Hanieh Moradian, Toralf Roch, Larissa Anthofer, Andreas Lendlein, Manfred Gossen

Publikováno v: Molecular Therapy: Nucleic Acids, Vol 27, Iss , Pp 854-869 (2022)

In vitro transcribed (IVT)-mRNA has been accepted as a promising therapeutic modality. Advances in facile and rapid production technologies make IVT-mRNA an appealing alternative to protein- or virus-based medicines. Robust expression levels, lack of

Externí odkaz: https://doaj.org/article/33098f5b6d044b3a88560070716004b2

mRNA Transfection-Induced Activation of Primary Human Monocytes and Macrophages: Dependence on Carrier System and Nucleotide Modification

Autor: Andreas Lendlein, Hanieh Moradian, Toralf Roch, Manfred Gossen

Publikováno v: Scientific Reports, Vol 10, Iss 1, Pp 1-15 (2020)
Moradian, H.; Roch, T.; Lendlein, A.; Gossen, M.: mRNA Transfection-Induced Activation of Primary Human Monocytes and Macrophages: Dependence on Carrier System and Nucleotide Modification. In: Scientific Reports. Vol. 10 (2020) 4181. (DOI: /10.1038/s41598-020-60506-4)
Scientific Reports

Monocytes and macrophages are key players in maintaining immune homeostasis. Identifying strategies to manipulate their functions via gene delivery is thus of great interest for immunological research and biomedical applications. We set out to establ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d04388814f38eae031e31bc26e10376c
http://link.springer.com/article/10.1038/s41598-020-60506-4

Strategies for simultaneous and successive delivery of RNA

Autor: Andreas Lendlein, Manfred Gossen, Hanieh Moradian

Publikováno v: Moradian, H.; Lendlein, A.; Gossen, M.: Strategies for simultaneous and successive delivery of RNA. In: Journal of Molecular Medicine. Vol. 98 (2020) 1767-1779. (DOI: /10.1007/s00109-020-01956-1)
Journal of Molecular Medicine (Berlin, Germany)

Advanced non-viral gene delivery experiments often require co-delivery of multiple nucleic acids. Therefore, the availability of reliable and robust co-transfection methods and defined selection criteria for their use in, e.g., expression of multimer

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d4cb94dae546daeb88d5f39ee17a0b3
https://publications.hzg.de/id/39217