Zobrazeno 1 - 4
of 4
pro vyhledávání: '"Hanh Thi-Thu Phan"'
Autor:
Ngan Thi Phuong Le, Trang Thi Phuong Phan, Hanh Thi Thu Phan, Tuom Thi Tinh Truong, Wolfgang Schumann, Hoang Duc Nguyen
Publikováno v:
Biotechnology Reports, Vol 35, Iss , Pp e00754- (2022)
The influence of fusion tags to produce recombinant proteins in the cytoplasm of Bacillus subtilis is not well-studied as in E. coli. This study aimed to investigate the influence of His-tags with different codons on the protein production levels of
Externí odkaz:
https://doaj.org/article/d02299ab317a4724bcade4e276cdbd09
Publikováno v:
Vietnam Journal of Science, Technology and Engineering, Vol 63, Iss 1 (2021)
An inducer-free expression vector for the low expression levels in Bacillus subtilis (B. subtilis) is necessary for fundamental research. In this study, we constructed inducer-free expression plasmids carrying Pspac, a well-known IPTG-inducible promo
Externí odkaz:
https://doaj.org/article/a3607a7d318f431db1cf2223a89a12f7
Publikováno v:
Vietnam Journal of Science, Technology and Engineering. 63:64-70
Construction of expression plasmid for Bacillus subtilis using Pspac promoter and BgaB as a reporter
Autor:
Phan Thi Phuong Trang, Chu Thi Bich Phuong, Nguyen Ngoc Yen Nhi, Le Thuy Tien, Hoang Duc Nguyen, Hanh Thi-Thu Phan, Le Thi Phuong Ngan
Publikováno v:
Science and Technology Development Journal. 22:239-246
Introduction: In basic research, it is essential to use an inducible promoter which can be controlled to express a small amount of protein for studying their roles in the cell. Pspac, a well-known weak promoter for Bacillus subtilis, uses isopropyl