Control of Microbial Opsin Expression in Stem Cell Derived Cones for Improved Outcomes in Cell Therapy

Autor: Marcela Garita-Hernandez, Antoine Chaffiol, Laure Guibbal, Fiona Routet, Hanen Khabou, Luisa Riancho, Lyes Toualbi, Serge Picaud, José-Alain Sahel, Olivier Goureau, Jens Duebel, Deniz Dalkara

Publikováno v: Frontiers in Cellular Neuroscience, Vol 15 (2021)

Human-induced pluripotent stem cell (hiPSC) derived organoids have become increasingly used systems allowing 3D-modeling of human organ development, and disease. They are also a reliable source of cells for transplantation in cell therapy and an exce

Externí odkaz: https://doaj.org/article/d52cba0458fb442b986f0c8aa3494476

All-optical inter-layers functional connectivity investigation in the mouse retina

Autor: Giulia Lia Beatrice Spampinato, Emiliano Ronzitti, Valeria Zampini, Ulisse Ferrari, Francesco Trapani, Hanen Khabou, Anurag Agraval, Deniz Dalkara, Serge Picaud, Eirini Papagiakoumou, Olivier Marre, Valentina Emiliani

Publikováno v: Cell reports methods. 2(8)

We developed a multi-unit microscope for all-optical inter-layers circuits interrogation. The system performs two-photon (2P) functional imaging and 2P multiplexed holographic optogenetics at axially distinct planes. We demonstrated the capability of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b237e9b166aad315485ee30ce86b6db4
https://pubmed.ncbi.nlm.nih.gov/36046629

Rescue of Defective Electroretinographic Responses in Dp71-Null Mice With AAV-Mediated Reexpression of Dp71

Autor: Dora Fix Ventura, Deniz Dalkara, Anneka Joachimsthaler, Jan Kremers, Lucile Vignaud, Hanen Khabou, Ophélie Vacca, Alvaro Rendon, Mirella Telles Salgueiro Barboni, Cyrille Vaillend, Andre Liber, Michel Roux, Xavier Guillonneau

Publikováno v: Investigative Ophthalmology & Visual Science
Investigative Ophthalmology & Visual Science, Association for Research in Vision and Ophthalmology, 2020, 61 (2), pp.11. ⟨10.1167/iovs.61.2.11⟩
Investigative Ophthalmology & Visual Science, 2020, 61 (2), pp.11. ⟨10.1167/iovs.61.2.11⟩

International audience; Purpose: To study the potential effect of a gene therapy, designed to rescue the expression of dystrophin Dp71 in the retinas of Dp71-null mice, on retinal physiology.Methods: We recorded electroretinograms (ERGs) in Dp71-null

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c023f52dde02bc90097185a7df09046
https://pubmed.ncbi.nlm.nih.gov/32049345

All-optical interrogation of a direction selective retinal circuit by holographic wave front shaping

Autor: Deniz Dalkara, Ulisse Ferrari, Emiliano Ronzitti, Emiliani, Serge Picaud, Eirini Papagiakoumou, Zampini, Trapani F, Hanen Khabou, Giulia Lb Spampinato, Olivier Marre

Direction selective (DS) ganglion cells (GC) in the retina maintain their tuning across a broad range of light levels. Yet very different circuits can shape their responses from bright to dim light, and their respective contributions are difficult to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::9cf77be32d63e7f0e65cc33ead989911
https://doi.org/10.1101/513192

Dosage Thresholds and Influence of Transgene Cassette in Adeno-Associated Virus–Related Toxicity

Autor: Hanen Khabou, Deniz Dalkara, Laure Pacot, Sylvain Fisson, Chloé Cordeau

Publikováno v: Human Gene Therapy
Human Gene Therapy, Mary Ann Liebert, 2018, ⟨10.1089/hum.2018.144⟩
Human Gene Therapy, Mary Ann Liebert, 2018, 0, ⟨10.1089/hum.2018.144⟩

International audience; Today, there are >500 published studies and 40 clinical trials to treat retinal disorders using gene therapy. The great majority of them rely on the use of adeno-associated virus vectors (AAV) for therapeutic gene delivery. Th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c63df2ea765c4bd1f25c805a75f3e84f
https://hal.sorbonne-universite.fr/hal-01927077