TIE: A Method to Electroporate Long DNA Templates into Preimplantation Embryos for CRISPR-Cas9 Gene Editing

Autor: Chiu-An Lo, Ibrahim Kays, Farida Emran, Hooman Bagheri, Xiang-Jiao Yang, Alan C. Peterson, Hana C. Friedman, Ellis Cooper, Katherine A. Siminovitch, Harry Shao, Yumaine Chong, Brian E. Chen

Publikováno v: The CRISPR Journal

Precise genome editing using CRISPR typically requires delivery of guide RNAs, Cas9 endonuclease, and DNA repair templates. Both microinjection and electroporation effectively deliver these components into mouse zygotes provided the DNA template is a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aefe79d2dc5b6119cb806da216b4adb6
https://doi.org/10.1089/crispr.2017.0020

Functional organization of an Mbp enhancer exposes striking transcriptional regulatory diversity within myelinating glia

Autor: Nancy, Dionne, Samar, Dib, Bente, Finsen, Eric, Denarier, Tanja, Kuhlmann, Régen, Drouin, Maia, Kokoeva, Thomas J, Hudson, Kathy, Siminovitch, Hana C, Friedman, Alan C, Peterson

Publikováno v: Glia. 64(1)

In mammals, large caliber axons are ensheathed by myelin, a glial specialization supporting axon integrity and conferring accelerated and energy-efficient action potential conduction. Myelin basic protein (MBP) is required for normal myelin elaborati

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::30813d9f74e954bd54ba13004981383a
https://pubmed.ncbi.nlm.nih.gov/26507463

A Distinct Pattern of Trophic Factor Expression in Myelin-Deficient Nerves ofTremblerMice: Implications for Trophic Support by Schwann Cells

Autor: Tony N. Jelsma, Garth M. Bray, Albert J. Aguayo, Hana C. Friedman

Publikováno v: The Journal of Neuroscience. 16:5344-5350

Distal to a peripheral nerve transection, myelin degradation and Schwann cell (SC) proliferation are accompanied by a marked upregulation of brain-derived neurotrophic factor (BDNF) and a decrease of ciliary neurotrophic factor (CNTF) in non-neuronal

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::656f9382714d4d2672dc6d3318b33c27
https://doi.org/10.1523/jneurosci.16-17-05344.1996

Integrin-mediated axoglial interactions initiate myelination in the central nervous system

Autor: Noboru H. Komiyama, Seth G. N. Grant, Peter J. Brophy, Zhen Wang, Alan C. Peterson, Joana Câmara, Charles ffrench-Constant, Diane L. Sherman, Matthew Grove, Cristina Nunes-Fonseca, Hana C. Friedman

Publikováno v: Câmara, J, Wang, Z, Nunes-Fonseca, C, Friedman, H C, Grove, M, Sherman, D L, Komiyama, N H, Grant, S G, Brophy, P J, Peterson, A & ffrench-Constant, C 2009, ' Integrin-mediated axoglial interactions initiate myelination in the central nervous system ', Journal of Cell Biology, vol. 185, no. 4, pp. 699-712 . https://doi.org/10.1083/jcb.200807010
The Journal of Cell Biology

All but the smallest-diameter axons in the central nervous system are myelinated, but the signals that initiate myelination are unknown. Our prior work has shown that integrin signaling forms part of the cell–cell interactions that ensure only thos

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::83877c0f05cc86d3ffdf93b7a54f5938
https://www.pure.ed.ac.uk/ws/files/7925520/Integrin_mediated_axoglial_interactions_initiate_myelination_in_the_central_nervous_system.pdf