Zobrazeno 1 - 10 of 12 pro vyhledávání: '"Haley R. Pugsley"'
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Akademický článek
Conventional, High-Resolution and Imaging Flow Cytometry: Benchmarking Performance in Characterisation of Extracellular Vesicles
Autor: Jaco Botha, Haley R. Pugsley, Aase Handberg
Publikováno v: Biomedicines, Vol 9, Iss 2, p 124 (2021)
Flow cytometry remains a commonly used methodology due to its ability to characterise multiple parameters on single particles in a high-throughput manner. In order to address limitations with lacking sensitivity of conventional flow cytometry to char
Externí odkaz: https://doaj.org/article/aac94592029d4a37b0b93d6b745fd9c8
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3
Integrated analysis of genotype and phenotype reveals clonal evolution and cytogenetically driven disruption of myeloid cell maturation in myelodysplastic syndromes
Autor: Nigar Zaidi, Richard Bennington, Haley R. Pugsley, Barbara K. Zehentner, Dongbin Xu, Timothy P. Singleton, Lisa Eidenschink Brodersen, Wayne Fritschle, Denise A. Wells, Andrew J. Menssen, Jevon Cutler, Luise Hartmann, Michael R. Loken
Publikováno v: Cytometry Part B: Clinical Cytometry. 104:183-194
BACKGROUND Myelodysplastic syndromes (MDS) are a heterogenous collection of clonal bone marrow diseases characterized by cytopenias, abnormal karyotypes, molecular abnormalities, and dysplasia by flow cytometry and/or morphology. The progression of M
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6144716259a498c58ffa84bf1d193738
https://doi.org/10.1002/cyto.b.22036
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4
Conventional, high-resolution and imaging flow cytometry:Benchmarking performance in characterisation of extracellular vesicles
Autor: Aase Handberg, Haley R. Pugsley, Jaco Botha
Publikováno v: Botha, J, Pugsley, H R & Handberg, A 2021, ' Conventional, high-resolution and imaging flow cytometry : Benchmarking performance in characterisation of extracellular vesicles ', Biomedicines, vol. 9, no. 2, 124 . https://doi.org/10.3390/biomedicines9020124
Biomedicines
Volume 9
Issue 2
Biomedicines, Vol 9, Iss 124, p 124 (2021)
Flow cytometry remains a commonly used methodology due to its ability to characterise multiple parameters on single particles in a high-throughput manner. In order to address limitations with lacking sensitivity of conventional flow cytometry to char
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6dcd8d8c7efad33ee2809738e57f3336
https://vbn.aau.dk/da/publications/4051fa4b-1d12-424d-a42e-7a4d36611eb0
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5
Quantifying autophagosomes and autolysosomes in cells using imaging flow cytometry
Autor: Ninh M. La-Beck, Haley R. Pugsley, Magdalena Karbowniczek, Robin Rajan, Manoj K. Sabnani, Aristotelis Astrinidis
Publikováno v: Cytometry Part A. 87:451-458
Autophagy dysregulation has been implicated in numerous diseases and many therapeutic agents are known to modulate this pathway. Therefore, the ability to accurately monitor autophagy is critical to understanding its role in the pathogenesis and trea
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::14f9df7e359646a4d1247c37a5fb80d3
https://doi.org/10.1002/cyto.a.22652
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6
Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
Autor: Haley R Pugsley
Publikováno v: Journal of Visualized Experiments : JoVE
Autophagy is a catabolic pathway in which normal or dysfunctional cellular components that accumulate during growth and differentiation are degraded via the lysosome and are recycled. During autophagy, cytoplasmic LC3 protein is lipidated and recruit
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::658a53a61697533d5997227cc7de72d0
http://europepmc.org/articles/PMC5612559
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7
Applications of Imaging Flow Cytometry for Microalgae
Autor: Mark, Hildebrand, Aubrey, Davis, Raffaela, Abbriano, Haley R, Pugsley, Jesse C, Traller, Sarah R, Smith, Roshan P, Shrestha, Orna, Cook, Eva L, Sánchez-Alvarez, Kalpana, Manandhar-Shrestha, Benjamin, Alderete
Publikováno v: Methods in molecular biology (Clifton, N.J.). 1389
The ability to image large numbers of cells at high resolution enhances flow cytometric analysis of cells and cell populations. In particular, the ability to image intracellular features adds a unique aspect to analyses, and can enable correlation be
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::6b00fded1912bf0842876deb6801f9d6
https://pubmed.ncbi.nlm.nih.gov/27460237
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8
Quantifying autophagy: Measuring LC3 puncta and autolysosome formation in cells using multispectral imaging flow cytometry
Autor: Haley R. Pugsley
Publikováno v: Methods (San Diego, Calif.). 112
The use of multispectral imaging flow cytometry has been gaining popularity due to its quantitative power, high throughput capabilities, multiplexing potential and its ability to acquire images of every cell. Autophagy is a process in which dysfuncti
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::903112b6991b6cefdecfc597f4f42996
https://pubmed.ncbi.nlm.nih.gov/27263026
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9
Applications of imaging flow cytometry for microalgae
Autor: Haley R. Pugsley, Mark Hildebrand, Kalpana Manandhar-Shrestha, Aubrey K. Davis, Raffaela M. Abbriano, Roshan P. Shrestha, Jesse C. Traller, Orna Cook, Benjamin Alderete, Eva L. Sánchez-Alvarez, Sarah R. Smith
Publikováno v: Imaging Flow Cytometry ISBN: 9781493933006
© Springer Science+Business Media New York 2016. The ability to image large numbers of cells at high resolution enhances flow cytometric analysis of cells and cell populations. In particular, the ability to image intracellular features adds a unique
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a21d13267d3ec87261ea2d63f202c0a6
https://hdl.handle.net/10453/118172
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10
Chemical cytometry: the chemical analysis of single cells
Autor: Colin D. Whitmore, Emily H. Turner, Daniella Cohen, Haley R. Pugsley, Norman J. Dovichi, Cuiru Zhu, David G. Gomez
Publikováno v: Analytical and Bioanalytical Chemistry. 390:223-226
The long-term goal of systems biology is to predict the response of an organism to a genetic or environmental change. However, such predictions are extremely challenging for whole organisms, even for the simplest organism. Instead, it is common to co
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bbc13dac2dde623635ea2cedeee5c0a3
https://doi.org/10.1007/s00216-007-1665-5
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