Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Hakim Kharrat"'
Autor:
Yassamine Lazrek, Olivier Dubreuil, Véronique Garambois, Nadège Gaborit, Christel Larbouret, Christophe Le Clorennec, Gaelle Thomas, Wilhem Leconet, Marta Jarlier, Martine Pugnière, Nadia Vié, Bruno Robert, Céline Monnet, Khalil Bouayadi, Hakim Kharrat, Philippe Mondon, André Pèlegrin, Thierry Chardès
Publikováno v:
Neoplasia: An International Journal for Oncology Research, Vol 15, Iss 3, Pp 335-347 (2013)
Blockade of the human epidermal growth factor receptor 3 (HER3) and of the downstream phosphatidylinositide 3-kinase (PI3K)/AKT pathway is a prerequisite for overcoming drug resistance and to develop novel treatments for cancers that are not eligible
Externí odkaz:
https://doaj.org/article/b4bb9ea6da264287898535708be0be8f
Autor:
Isabelle André, Magali Remaud-Simeon, Philippe Mondon, Hakim Kharrat, Gabrielle Potocki-Véronèse, Kais Jaziri, Khalil Bouayadi, Pierre Monsan, Stéphane Emond
Publikováno v:
Protein Science. 17:967-976
Amylosucrase is a transglucosidase that catalyzes amylose-like polymer synthesis from sucrose substrate. About 60,000 amylosucrase variants from two libraries generated by the MutaGen random mutagenesis method were submitted to an in vivo selection p
Autor:
Fabien Crozet, Magali Remaud-Simeon, Hakim Kharrat, Philippe Mondon, Gabrielle Potocki-Véronèse, Sandra Pizzut-Serin, Pierre Monsan, Khalil Bouayadi, Laurent Douchy, Stéphane Emond
Publikováno v:
Protein Engineering, Design and Selection. 21:267-274
The in vitro MutaGen procedure is a new random mutagenesis method based on the use of low-fidelity DNA polymerases. In the present study, this technique was applied on a 2 kb gene encoding amylosucrase, an attractive enzyme for the industrial synthes
Autor:
Magali Remaud-Simeon, Hakim Kharrat, Stéphane Emond, Pierre Monsan, Philippe Mondon, Khalil Bouayadi, Gabrielle Potocki-Veronese
Publikováno v:
SLAS Discovery. 12:715-723
This article describes the design and validation of a general procedure for the high-throughput isolation of amylosucrase variants displaying higher thermostability or increased resistance to organic solvents. This procedure consists of 2 successive
Autor:
Fabien Crozet, Hakim Kharrat, Nathalie Souyris, Philippe Mondon, Khalil Bouayadi, Laurent Douchy
Publikováno v:
Biotechnology Journal. 2:76-82
The selection of antibody fragments from libraries using in vitro screening technologies has proven to be a very good alternative to the classical hybridoma technology, and has overcome the laborious process of antibody humanization. However, the com
Autor:
Laurence, Renaut, Céline, Monnet, Olivier, Dubreuil, Ouafa, Zaki, Fabien, Crozet, Khalil, Bouayadi, Hakim, Kharrat, Philippe, Mondon
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 907
As a growing number of therapeutic antibodies are developed, robust methods to efficiently improve the affinity and/or specificity of antibody candidates are needed. Here we describe our powerful platform that combines scFv affinity maturation and Ig
Autor:
Khalil Bouayadi, Hakim Kharrat, Philippe Mondon, Ouafa Zaki, Fabien Crozet, Olivier Dubreuil, Céline Monnet, Laurence Renaut
Publikováno v:
Antibody Engineering ISBN: 9781617799730
As a growing number of therapeutic antibodies are developed, robust methods to efficiently improve the affinity and/or specificity of antibody candidates are needed. Here we describe our powerful platform that combines scFv affinity maturation and Ig
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6b1b455fb18db80bf2f3a711a2d2eb5c
https://doi.org/10.1007/978-1-61779-974-7_26
https://doi.org/10.1007/978-1-61779-974-7_26
Autor:
Philippe, Mondon, David, Grand, Nathalie, Souyris, Stéphane, Emond, Khalil, Bouayadi, Hakim, Kharrat
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 634
Random mutagenesis is one of the most effective methodologies to generate variant libraries for directed protein evolution. Indeed, this approach requires no structural or mechanistic information and can uncover unexpected beneficial mutations. Here,
Autor:
Hakim Kharrat, Philippe Mondon, Khalil Bouayadi, Nathalie Souyris, Stéphane Emond, David Grand
Publikováno v:
Methods in Molecular Biology ISBN: 9781607616511
Random mutagenesis is one of the most effective methodologies to generate variant libraries for directed protein evolution. Indeed, this approach requires no structural or mechanistic information and can uncover unexpected beneficial mutations. Here,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::de08d0b6540ba29309b1022e70a79f99
https://doi.org/10.1007/978-1-60761-652-8_26
https://doi.org/10.1007/978-1-60761-652-8_26
Publikováno v:
Analytical biochemistry. 384(2)
One approach to drug discovery involves the targeting of abnormal protein-protein interactions that lead to pathology. We present a new technology allowing the detection of such interactions within the cytoplasm in a yeast-based system. The interacti