Directed evolution of piperazic acid incorporation by a nonribosomal peptide synthetase

Autor: Philipp Stephan, Chloe Langley, Daniela Winkler, Jérôme Basquin, Lorenzo Caputi, Sarah E. O’Connor, Hajo Kries

Publikováno v: Angewandte Chemie International Edition

Engineering of biosynthetic enzymes is increasingly employed to synthesize structural analogues of antibiotics. Of special interest are non-ribosomal peptide synthetases (NRPSs) responsible for production of important antimicrobial peptides. Here, di

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d81461b0437bd3d712d12dd31e8320c1
https://hdl.handle.net/21.11116/0000-000D-4CA3-621.11116/0000-000D-4CA5-421.11116/0000-000D-4CA6-3

Mikrobielle Antibiotikafabriken verstehen und verbessern

Autor: Markus Gressler, Hajo Kries

Publikováno v: BIOspektrum. 28:481-483

Repurposing the enzymes in microbial metabolism such as nonribosomal peptide synthetases (NRPSs) is explored as a route towards better antibiotics. NRPSs are gigantic enzymatic assembly lines that form highly modified peptides from diverse building b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3b307360a0c526bc2ca130908e478542
https://doi.org/10.1007/s12268-022-1810-x

Total Synthesis and Functional Evaluation of IORs, Sulfonolipid‐based Inhibitors of Cell Differentiation in Salpingoeca rosetta

Autor: Luka Raguž, Chia‐Chi Peng, Florentine U. N. Rutaganira, Thomas Krüger, Aleksa Stanišić, Theresa Jautzus, Hajo Kries, Olaf Kniemeyer, Axel A. Brakhage, Nicole King, Christine Beemelmanns

Publikováno v: Angewandte Chemie. 134

The choanoflagellate Salpingoeca rosetta is an important model system to study the evolution of multicellularity. In this study we developed a new, modular, and scalable synthesis of sulfonolipid IOR-1A (six steps, 27 % overall yield), which acts as

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::40078d99698845105caa4ced83b55d4d
https://doi.org/10.1002/ange.202209105

Sulfonium Acids Loaded onto an Unusual Thiotemplate Assembly Line Construct the Cyclopropanol Warhead of a Burkholderia Virulence Factor

Autor: Jakob Franke, Keishi Ishida, Hajo Kries, Georg Pohnert, Mie Ishida-Ito, Felix Trottmann, Christian Hertweck, Aleksa Stanišić

Publikováno v: Angewandte Chemie-International Edition 59 (2020), Nr. 32
Angewandte Chemie (International Ed. in English)
Angewandte Chemie International Edition

Pathogenic bacteria of the Burkholderia pseudomallei group cause severe infectious diseases such as glanders and melioidosis. Malleicyprols were identified as important bacterial virulence factors, yet the biosynthetic origin of their cyclopropanol w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8366f9a0f88a8eb609e9062d86143ef1
https://doi.org/10.1002/anie.202003958

Emergence of a Negative Activation Heat Capacity during Evolution of a Designed Enzyme

Autor: Luca Marchetti, Hajo Kries, Adrian J. Mulholland, Cathleen Zeymer, Donald Hilvert, Peer R. E. Mittl, H. Adrian Bunzel

Publikováno v: Bunzel, A, Kries, H, Marchetti, L, Zeymer, C, Mittl, P R E, Mulholland, A & Hilvert, D 2019, ' Emergence of a Negative Activation Heat Capacity during Evolution of a Designed Enzyme ', Journal of the American Chemical Society, vol. 141, no. 30, pp. 11745-11748 . https://doi.org/10.1021/jacs.9b02731

Temperature influences the reaction kinetics and evolvability of all enzymes. To understand how evolution shapes the thermodynamic drivers of catalysis, we optimized the modest activity of a computationally designed enzyme for an elementary proton-tr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7478a6e693922bc4d56e34d60bdf4935
https://doi.org/10.1021/jacs.9b02731