Diagnostic predictive values of the Hain genotype MTBDRsl assay in mycobacterial strains isolated from Sudan

Autor: Muatsim Ahmed Mohammed Adam, Hamdan Mustafa Hamdan Ali, Eltahir Awad Gasim Khalil

Publikováno v: The Pan African Medical Journal, Vol 32, Iss 124 (2019)

INTRODUCTION: Hain GenoType MTBDRsl is nucleic acid amplification assay based on reverse hybridization with specific oligonucleotide probes on nitrocellulose strips. MTBDRsl identifies M. tuberculosis complex and detects resistance to fluoroquinolone

Externí odkaz: https://doaj.org/article/f3b8cdaaae7f49bf99d4012edd1f964d

Some Synonymous and Nonsynonymous gyrA Mutations in Mycobacterium tuberculosis Lead to Systematic False-Positive Fluoroquinolone Resistance Results with the Hain GenoType MTBDRsl Assays

Autor: Ajileye, Adebisi, Alvarez, Nataly, Merker, Matthias, Walker, Timothy M., Akter, Suriya, Brown, Kerstin, Moradigaravand, Danesh, Schön, Thomas, Andres, Sönke, Schleusener, Viola, Omar, Shaheed V., Coll, Francesc, Huang, Hairong, Diel, Roland, Ismail, Nazir, Parkhill, Julian, de Jong, Bouke C., Peto, Tim E. A., Crook, Derrick W., Niemann, Stefan, Robledo, Jaime, Smith, E. Grace, Peacock, Sharon J., Köser, Claudio U.

Publikováno v: Antimicrobial Agents and Chemotherapy

In this study, using the Hain GenoType MTBDRsl assays (versions 1 and 2), we found that some nonsynonymous and synonymous mutations in gyrA in Mycobacterium tuberculosis result in systematic false-resistance results to fluoroquinolones by preventing

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::819162a67226bd53265a17f66a4938ea
http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-136869

Some Synonymous and Nonsynonymous gyrA Mutations in Mycobacterium tuberculosis Lead to Systematic False-Positive Fluoroquinolone Resistance Results with the Hain GenoType MTBDR sl Assays.

Autor: Ajileye A; Public Health England West Midlands Public Health Laboratory, Heartlands Hospital, Birmingham, United Kingdom., Alvarez N; Bacteriology and Mycobacteria Unit, Corporación Para Investigaciones Biológicas, Medellín, Colombia.; Universidad Pontificia Bolivariana, Medellín, Colombia., Merker M; Division of Molecular and Experimental Mycobacteriology, Research Center Borstel, Borstel, Germany.; German Center for Infection Research (DZIF), Partnersite Hamburg-Lübeck-Borstel, Borstel, Germany., Walker TM; Nuffield Department of Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom., Akter S; Mycobacteriology Unit, Department of Microbiology, Institute of Tropical Medicine, Antwerp, Belgium., Brown K; Public Health England West Midlands Public Health Laboratory, Heartlands Hospital, Birmingham, United Kingdom., Moradigaravand D; Wellcome Trust Sanger Institute, Hinxton, United Kingdom., Schön T; Department of Clinical and Experimental Medicine, Division of Medical Microbiology, Linköping University, Linköping, Sweden.; Department of Clinical Microbiology and Infectious Diseases, Kalmar County Hospital, Kalmar, Sweden., Andres S; Division of Mycobacteriology, National Tuberculosis Reference Laboratory, Research Center Borstel, Borstel, Germany., Schleusener V; Division of Molecular and Experimental Mycobacteriology, Research Center Borstel, Borstel, Germany., Omar SV; Centre for Tuberculosis, National Institute for Communicable Diseases, Johannesburg, South Africa., Coll F; London School of Hygiene & Tropical Medicine, London, United Kingdom., Huang H; National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory on Drug-Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing, China., Diel R; Institute of Epidemiology, University Hospital Schleswig-Holstein, Campus Kiel, Kiel, Germany., Ismail N; Centre for Tuberculosis, National Institute for Communicable Diseases, Johannesburg, South Africa., Parkhill J; Wellcome Trust Sanger Institute, Hinxton, United Kingdom., de Jong BC; Mycobacteriology Unit, Department of Microbiology, Institute of Tropical Medicine, Antwerp, Belgium., Peto TE; Nuffield Department of Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom., Crook DW; Nuffield Department of Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom.; Public Health England, Microbiology Services, London, United Kingdom., Niemann S; Division of Molecular and Experimental Mycobacteriology, Research Center Borstel, Borstel, Germany.; German Center for Infection Research (DZIF), Partnersite Hamburg-Lübeck-Borstel, Borstel, Germany., Robledo J; Bacteriology and Mycobacteria Unit, Corporación Para Investigaciones Biológicas, Medellín, Colombia.; Universidad Pontificia Bolivariana, Medellín, Colombia., Smith EG; Public Health England West Midlands Public Health Laboratory, Heartlands Hospital, Birmingham, United Kingdom., Peacock SJ; Wellcome Trust Sanger Institute, Hinxton, United Kingdom.; London School of Hygiene & Tropical Medicine, London, United Kingdom.; Department of Medicine, University of Cambridge, Cambridge, United Kingdom., Köser CU; Department of Medicine, University of Cambridge, Cambridge, United Kingdom cuk21@cam.ac.uk.

Publikováno v: Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2017 Mar 24; Vol. 61 (4). Date of Electronic Publication: 2017 Mar 24 (Print Publication: 2017).