Plasmodium falciparum African PfCRT Mutant Isoforms Conducive to Piperaquine Resistance are Infrequent and Impart a Major Fitness Cost.

Autor: Hagenah LM; Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, NY 10032, USA.; Center for Malaria Therapeutics and Antimicrobial Resistance, Columbia University Irving Medical Center, New York, NY, USA., Yeo T; Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, NY 10032, USA.; Center for Malaria Therapeutics and Antimicrobial Resistance, Columbia University Irving Medical Center, New York, NY, USA., Schindler KA; Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, NY 10032, USA.; Center for Malaria Therapeutics and Antimicrobial Resistance, Columbia University Irving Medical Center, New York, NY, USA., Jeon JH; Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, NY 10032, USA.; Center for Malaria Therapeutics and Antimicrobial Resistance, Columbia University Irving Medical Center, New York, NY, USA., Bloxham TS; Center for Malaria Therapeutics and Antimicrobial Resistance, Columbia University Irving Medical Center, New York, NY, USA.; Division of Infectious Diseases, Department of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USA., Small-Saunders JL; Center for Malaria Therapeutics and Antimicrobial Resistance, Columbia University Irving Medical Center, New York, NY, USA.; Division of Infectious Diseases, Department of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USA., Mok S; Center for Malaria Therapeutics and Antimicrobial Resistance, Columbia University Irving Medical Center, New York, NY, USA.; Division of Infectious Diseases, Department of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USA., Fidock DA; Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, NY 10032, USA.; Center for Malaria Therapeutics and Antimicrobial Resistance, Columbia University Irving Medical Center, New York, NY, USA.; Division of Infectious Diseases, Department of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USA.

Publikováno v: The Journal of infectious diseases [J Infect Dis] 2024 Dec 11. Date of Electronic Publication: 2024 Dec 11.

Additional PfCRT mutations driven by selective pressure for improved fitness can result in the loss of piperaquine resistance and altered Plasmodium falciparum physiology.

Autor: Hagenah LM; Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, New York, USA.; Center for Malaria Therapeutics and Antimicrobial Resistance, Columbia University Irving Medical Center, New York, New York, USA., Dhingra SK; Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, New York, USA.; Center for Malaria Therapeutics and Antimicrobial Resistance, Columbia University Irving Medical Center, New York, New York, USA., Small-Saunders JL; Center for Malaria Therapeutics and Antimicrobial Resistance, Columbia University Irving Medical Center, New York, New York, USA.; Division of Infectious Diseases, Department of Medicine, Columbia University Irving Medical Center, New York, New York, USA., Qahash T; Department of Chemistry, Pennsylvania State University, University Park, Pennsylvania, USA.; Department of Biochemistry and Molecular Biology and Huck Center for Malaria Research, Pennsylvania State University, University Park, Pennsylvania, USA., Willems A; Department of Chemistry, Georgetown University, Washington, DC, USA.; Department of Biochemistry and Cellular and Molecular Biology, Georgetown University, Washington, DC, USA., Schindler KA; Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, New York, USA.; Center for Malaria Therapeutics and Antimicrobial Resistance, Columbia University Irving Medical Center, New York, New York, USA., Rangel GW; Department of Biochemistry and Molecular Biology and Huck Center for Malaria Research, Pennsylvania State University, University Park, Pennsylvania, USA., Gil-Iturbe E; Department of Psychiatry, Columbia University Irving Medical Center, New York, New York, USA., Kim J; Department of Physiology and Cellular Biophysics, Columbia University Irving Medical Center, New York, New York, USA., Akhundova E; Department of Physiology and Cellular Biophysics, Columbia University Irving Medical Center, New York, New York, USA., Yeo T; Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, New York, USA.; Center for Malaria Therapeutics and Antimicrobial Resistance, Columbia University Irving Medical Center, New York, New York, USA., Okombo J; Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, New York, USA.; Center for Malaria Therapeutics and Antimicrobial Resistance, Columbia University Irving Medical Center, New York, New York, USA., Mancia F; Department of Physiology and Cellular Biophysics, Columbia University Irving Medical Center, New York, New York, USA., Quick M; Department of Psychiatry, Columbia University Irving Medical Center, New York, New York, USA.; Department of Physiology and Cellular Biophysics, Columbia University Irving Medical Center, New York, New York, USA.; Area Neuroscience - Molecular Therapeutics, New York State Psychiatric Institute, New York, New York, USA., Roepe PD; Department of Chemistry, Georgetown University, Washington, DC, USA.; Department of Biochemistry and Cellular and Molecular Biology, Georgetown University, Washington, DC, USA., Llinás M; Department of Chemistry, Pennsylvania State University, University Park, Pennsylvania, USA.; Department of Biochemistry and Molecular Biology and Huck Center for Malaria Research, Pennsylvania State University, University Park, Pennsylvania, USA., Fidock DA; Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, New York, USA.; Center for Malaria Therapeutics and Antimicrobial Resistance, Columbia University Irving Medical Center, New York, New York, USA.; Division of Infectious Diseases, Department of Medicine, Columbia University Irving Medical Center, New York, New York, USA.

Publikováno v: MBio [mBio] 2024 Jan 16; Vol. 15 (1), pp. e0183223. Date of Electronic Publication: 2023 Dec 07.

Keystone Malaria Symposium 2022: a vibrant discussion of progress made and challenges ahead from drug discovery to treatment.

Autor: Kanai M; Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, NY, USA. Electronic address: mk3593@cumc.columbia.edu., Hagenah LM; Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, NY, USA. Electronic address: lmh2220@cumc.columbia.edu., Ashley EA; Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, UK; Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Mahosot Hospital, Vientiane, Laos. Electronic address: liz@tropmedres.ac., Chibale K; Drug Discovery and Development (H3D) Centre, University of Cape Town, Rondebosch, South Africa; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa; South African Medical Research Council Drug Discovery and Development Research Unit, University of Cape Town, Rondebosch, South Africa; Department of Chemistry, University of Cape Town, Rondebosch, South Africa. Electronic address: kelly.chibale@uct.ac.za., Fidock DA; Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, NY, USA; Center for Malaria Therapeutics and Antimicrobial Resistance, Division of Infectious Diseases, Department of Medicine, Columbia University Irving Medical Center, New York, NY, USA. Electronic address: df2260@cumc.columbia.edu.

Publikováno v: Trends in parasitology [Trends Parasitol] 2022 Sep; Vol. 38 (9), pp. 711-718. Date of Electronic Publication: 2022 Jul 18.

Turning the tide: targeting PfCRT to combat drug-resistant P. falciparum?

Autor: Small-Saunders JL; Division of Infectious Diseases, Department of Medicine, Columbia University Irving Medical Center, New York, NY, USA., Hagenah LM; Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, NY, USA., Fidock DA; Division of Infectious Diseases, Department of Medicine, Columbia University Irving Medical Center, New York, NY, USA. df2260@cumc.columbia.edu.; Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, NY, USA. df2260@cumc.columbia.edu.

Publikováno v: Nature reviews. Microbiology [Nat Rev Microbiol] 2020 May; Vol. 18 (5), pp. 261-262.

Structure and drug resistance of the Plasmodium falciparum transporter PfCRT.

Autor: Kim J; Department of Physiology and Cellular Biophysics, Columbia University Irving Medical Center, New York, NY, USA., Tan YZ; Department of Physiology and Cellular Biophysics, Columbia University Irving Medical Center, New York, NY, USA.; National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA., Wicht KJ; Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, NY, USA., Erramilli SK; Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL, USA., Dhingra SK; Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, NY, USA., Okombo J; Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, NY, USA., Vendome J; Schrödinger, New York, NY, USA., Hagenah LM; Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, NY, USA., Giacometti SI; Department of Physiology and Cellular Biophysics, Columbia University Irving Medical Center, New York, NY, USA., Warren AL; Department of Psychiatry, Columbia University Irving Medical Center, New York, NY, USA., Nosol K; Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL, USA., Roepe PD; Department of Chemistry, Georgetown University, Washington, DC, USA.; Department of Biochemistry and Cellular and Molecular Biology, Georgetown University, Washington, DC, USA., Potter CS; National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.; Department of Biochemistry and Molecular Biophysics, Columbia University Irving Medical Center, New York, NY, USA., Carragher B; National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.; Department of Biochemistry and Molecular Biophysics, Columbia University Irving Medical Center, New York, NY, USA., Kossiakoff AA; Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL, USA., Quick M; Department of Psychiatry, Columbia University Irving Medical Center, New York, NY, USA. mq2102@cumc.columbia.edu.; Center for Molecular Recognition, Columbia University Irving Medical Center, New York, NY, USA. mq2102@cumc.columbia.edu.; Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, NY, USA. mq2102@cumc.columbia.edu., Fidock DA; Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York, NY, USA. df2260@cumc.columbia.edu.; Division of Infectious Diseases, Department of Medicine, Columbia University Irving Medical Center, New York, NY, USA. df2260@cumc.columbia.edu., Mancia F; Department of Physiology and Cellular Biophysics, Columbia University Irving Medical Center, New York, NY, USA. fm123@cumc.columbia.edu.

Publikováno v: Nature [Nature] 2019 Dec; Vol. 576 (7786), pp. 315-320. Date of Electronic Publication: 2019 Nov 27.