Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Hagen U Tilgner"'
Autor:
Binsheng Gong, Dan Li, Paweł P. Łabaj, Bohu Pan, Natalia Novoradovskaya, Danielle Thierry-Mieg, Jean Thierry-Mieg, Guangchun Chen, Anne Bergstrom Lucas, Jennifer S. LoCoco, Todd A. Richmond, Elizabeth Tseng, Rebecca Kusko, Scott Happe, Timothy R. Mercer, Carlos Pabón-Peña, Michael Salmans, Hagen U. Tilgner, Wenzhong Xiao, Donald J. Johann, Wendell Jones, Weida Tong, Christopher E. Mason, David P. Kreil, Joshua Xu
Publikováno v:
Scientific Data, Vol 11, Iss 1, Pp 1-13 (2024)
Abstract Next-generation sequencing (NGS) has revolutionized genomic research by enabling high-throughput, cost-effective genome and transcriptome sequencing accelerating personalized medicine for complex diseases, including cancer. Whole genome/tran
Externí odkaz:
https://doaj.org/article/73cc5c3ee70441e7acfa9bce7c026093
Autor:
Matthew T. Biegler, Olivier Fedrigo, Paul Collier, Jacquelyn Mountcastle, Bettina Haase, Hagen U. Tilgner, Erich D. Jarvis
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-17 (2022)
Abstract The zebra finch is one of the most commonly studied songbirds in biology, particularly in genomics, neuroscience and vocal communication. However, this species lacks a robust cell line for molecular biology research and reagent optimization.
Externí odkaz:
https://doaj.org/article/20f85c97724d4ac290a1a219b9a09e84
A spatially resolved brain region- and cell type-specific isoform atlas of the postnatal mouse brain
Autor:
Anoushka Joglekar, Andrey Prjibelski, Ahmed Mahfouz, Paul Collier, Susan Lin, Anna Katharina Schlusche, Jordan Marrocco, Stephen R. Williams, Bettina Haase, Ashley Hayes, Jennifer G. Chew, Neil I. Weisenfeld, Man Ying Wong, Alexander N. Stein, Simon A. Hardwick, Toby Hunt, Qi Wang, Christoph Dieterich, Zachary Bent, Olivier Fedrigo, Steven A. Sloan, Davide Risso, Erich D. Jarvis, Paul Flicek, Wenjie Luo, Geoffrey S. Pitt, Adam Frankish, August B. Smit, M. Elizabeth Ross, Hagen U. Tilgner
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021)
Alternative RNA splicing varies across the brain. Its mapping at single cell resolution is unclear. Here, the authors provide a spatial and single-cell splicing atlas reporting brain region- and cell type-specific expression of different isoforms in
Externí odkaz:
https://doaj.org/article/b27a2afb47544e009093959c837b41e3
Autor:
Iman Hajirasouliha, Hagen U. Tilgner
Publikováno v:
Genome Biology, Vol 20, Iss 1, Pp 1-4 (2019)
Abstract The 19th Annual Advances in Genome Biology and Technology (AGBT) meeting came back to Marco Island, Florida, and was held in the renovated venue from 27 February to 2 March 2019. The meeting showed a variety of new technology, both in wet la
Externí odkaz:
https://doaj.org/article/05622b6804ce423abebf61e046f7bb2e
Autor:
Careen Foord, Justine Hsu, Julien Jarroux, Wen Hu, Natan Belchikov, Shaun Pollard, Yi He, Anoushka Joglekar, Hagen U. Tilgner
Publikováno v:
Nature Methods. 20:20-24
Autor:
Simon A. Hardwick, Wen Hu, Anoushka Joglekar, Li Fan, Paul G. Collier, Careen Foord, Jennifer Balacco, Samantha Lanjewar, Maureen McGuirk Sampson, Frank Koopmans, Andrey D. Prjibelski, Alla Mikheenko, Natan Belchikov, Julien Jarroux, Anne Bergstrom Lucas, Miklós Palkovits, Wenjie Luo, Teresa A. Milner, Lishomwa C. Ndhlovu, August B. Smit, John Q. Trojanowski, Virginia M. Y. Lee, Olivier Fedrigo, Steven A. Sloan, Dóra Tombácz, M. Elizabeth Ross, Erich Jarvis, Zsolt Boldogkői, Li Gan, Hagen U. Tilgner
Publikováno v:
Nature Biotechnology, 40(7), 1082-1092. Nature Publishing Group
Hardwick, S A, Hu, W, Joglekar, A, Fan, L, Collier, P G, Foord, C, Balacco, J, Lanjewar, S, Sampson, M M G, Koopmans, F, Prjibelski, A D, Mikheenko, A, Belchikov, N, Jarroux, J, Lucas, A B, Palkovits, M, Luo, W, Milner, T A, Ndhlovu, L C, Smit, A B, Trojanowski, J Q, Lee, V M Y, Fedrigo, O, Sloan, S A, Tombácz, D, Ross, M E, Jarvis, E, Boldogkői, Z, Gan, L & Tilgner, H U 2022, ' Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue ', Nature Biotechnology, vol. 40, no. 7, pp. 1082-1092 . https://doi.org/10.1038/s41587-022-01231-3
Hardwick, S A, Hu, W, Joglekar, A, Fan, L, Collier, P G, Foord, C, Balacco, J, Lanjewar, S, Sampson, M M G, Koopmans, F, Prjibelski, A D, Mikheenko, A, Belchikov, N, Jarroux, J, Lucas, A B, Palkovits, M, Luo, W, Milner, T A, Ndhlovu, L C, Smit, A B, Trojanowski, J Q, Lee, V M Y, Fedrigo, O, Sloan, S A, Tombácz, D, Ross, M E, Jarvis, E, Boldogkői, Z, Gan, L & Tilgner, H U 2022, ' Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue ', Nature Biotechnology, vol. 40, no. 7, pp. 1082-1092 . https://doi.org/10.1038/s41587-022-01231-3
Single-nuclei RNA sequencing characterizes cell types at the gene level. However, compared to single-cell approaches, many single-nuclei cDNAs are purely intronic, lack barcodes and hinder the study of isoforms. Here we present single-nuclei isoform
Publikováno v:
Frontiers in Genetics, Vol 10 (2019)
The advent of second-generation sequencing and its application to RNA sequencing have revolutionized the field of genomics by allowing quantification of gene expression, as well as the definition of transcription start/end sites, exons, splice sites
Externí odkaz:
https://doaj.org/article/7487948fcfe9413eb62ae30b8e360ecc
Autor:
Simon A. Hardwick, Samuel D. Bassett, Dominik Kaczorowski, James Blackburn, Kirston Barton, Nenad Bartonicek, Shaun L. Carswell, Hagen U. Tilgner, Clement Loy, Glenda Halliday, Tim R. Mercer, Martin A. Smith, John S. Mattick
Publikováno v:
Frontiers in Genetics, Vol 10 (2019)
The human brain is one of the last frontiers of biomedical research. Genome-wide association studies (GWAS) have succeeded in identifying thousands of haplotype blocks associated with a range of neuropsychiatric traits, including disorders such as sc
Externí odkaz:
https://doaj.org/article/1e92fdf57727448c8d717531c35b6024
Autor:
Anoushka Joglekar, Wen Hu, Bei Zhang, Oleksandr Narykov, Mark Diekhans, Jennifer Balacco, Lishomwa C Ndhlovu, Teresa A Milner, Olivier Fedrigo, Erich D Jarvis, Gloria Sheynkman, Dmitry Korkin, M. Elizabeth Ross, Hagen U. Tilgner
RNA isoforms influence cell identity and function. Until recently, technological limitations prevented a genome-wide appraisal of isoform influence on cell identity in various parts of the brain. Using enhanced long-read single-cell isoform sequencin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::00cf03d4f20784df665358b6066d9981
https://doi.org/10.1101/2023.04.02.535281
https://doi.org/10.1101/2023.04.02.535281
Autor:
Andrey D. Prjibelski, Alla Mikheenko, Anoushka Joglekar, Alexander Smetanin, Julien Jarroux, Alla L. Lapidus, Hagen U. Tilgner
Publikováno v:
Nature Biotechnology.
Annotating newly sequenced genomes and determining alternative isoforms from long-read RNA data are complex and incompletely solved problems. Here we present IsoQuant—a computational tool using intron graphs that accurately reconstructs transcripts