Enhanced ER protein processing gene expression increases rAAV yield and full capsid ratio in HEK293 cells.

Autor: Fu Q; Department of Biomedical Engineering and Biotechnology, University of Massachusetts Lowell, Lowell, MA, 01854, USA., Wang Y; Department of Chemical Engineering, University of Massachusetts Lowell, Lowell, MA, 01854, USA., Qin J; Department of Biomedical Engineering and Biotechnology, University of Massachusetts Lowell, Lowell, MA, 01854, USA., Xie D; Department of Chemical Engineering, University of Massachusetts Lowell, Lowell, MA, 01854, USA., McNally D; Department of Chemical Engineering, University of Massachusetts Lowell, Lowell, MA, 01854, USA.; MassBiologics, University of Massachusetts Chan Medical School, Mattapan, MA, 02126, USA., Yoon S; Department of Biomedical Engineering and Biotechnology, University of Massachusetts Lowell, Lowell, MA, 01854, USA. seongkyu_yoon@uml.edu.; Department of Chemical Engineering, University of Massachusetts Lowell, Lowell, MA, 01854, USA. seongkyu_yoon@uml.edu.

Publikováno v: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2024 Sep 04; Vol. 108 (1), pp. 459. Date of Electronic Publication: 2024 Sep 04.

[Knockout of RIG-I in HEK293 cells by CRISPR/Cas9].

Autor: Chen Z; College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, Zhejiang, China., Wu Y; College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, Zhejiang, China., Zhang Y; College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, Zhejiang, China., Gao Y; College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, Zhejiang, China.

Publikováno v: Sheng wu gong cheng xue bao = Chinese journal of biotechnology [Sheng Wu Gong Cheng Xue Bao] 2024 Nov 25; Vol. 40 (11), pp. 4254-4265.

Calcium oscillations in HEK293 cells lacking SOCE suggest the existence of a balanced regulation of IP3 production and degradation.

Autor: Octors, Clara¹, Yoast, Ryan E.², Emrich, Scott M.², Trebak, Mohamed^3,4,5, Sneyd, James⁶ sneyd@math.auckland.ac.nz

Publikováno v: Frontiers in Systems Biology. 2024, p1-10. 10p.

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Internalization of PEI-based complexes in transient transfection of HEK293 cells is triggered by coalescence of membrane heparan sulfate proteoglycans like Glypican-4.

Autor: Pérez-Rubio P; Grup d'Enginyeria de Bioprocessos i Biocatàlisi Aplicada, Escola d'Enginyeria, Universitat Autònoma de Barcelona, Campus de Bellaterra, Cerdanyola del Vallès, Barcelona 08193, Spain., Vendrell-Flotats M; Servei de Microscòpia, Universitat Autònoma de Barcelona, Campus de Bellaterra, Cerdanyola del Vallès, Barcelona 08193, Spain., Romero EL; Grup d'Enginyeria de Bioprocessos i Biocatàlisi Aplicada, Escola d'Enginyeria, Universitat Autònoma de Barcelona, Campus de Bellaterra, Cerdanyola del Vallès, Barcelona 08193, Spain., Enemark-Rasmussen K; Department of Chemistry, Technical University of Denmark, Kongens Lyngby, Denmark., Cervera L; Grup d'Enginyeria de Bioprocessos i Biocatàlisi Aplicada, Escola d'Enginyeria, Universitat Autònoma de Barcelona, Campus de Bellaterra, Cerdanyola del Vallès, Barcelona 08193, Spain; Serra Hunter Lecturer Professor. Electronic address: laura.cervera@uab.cat., Gòdia F; Grup d'Enginyeria de Bioprocessos i Biocatàlisi Aplicada, Escola d'Enginyeria, Universitat Autònoma de Barcelona, Campus de Bellaterra, Cerdanyola del Vallès, Barcelona 08193, Spain., Lavado-García J; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby Kgs 2800, Denmark. Electronic address: jlavgar@dtu.dk.

Publikováno v: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2024 Jul; Vol. 176, pp. 116893. Date of Electronic Publication: 2024 Jun 07.