Zobrazeno 1 - 10
of 28
pro vyhledávání: '"H.P.J. Bonarius"'
Autor:
Georg Schmid, H.P.J. Bonarius, Johannes Tramper, Ahmet Ozemre, Bram Timmerarends, Elmar Heinzle, Peter Skrabal
Publikováno v:
Biotechnology and Bioengineering. 74:528-538
Protein production of mammalian-cell culture is limited due to accumulation of waste products such as lactate, CO2, and ammonia. In this study, the intracellular fluxes of hybridoma cells are measured to determine the amount by which various metaboli
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::da84382847d8437ad0a0112746db1789
https://doi.org/10.1002/bit.1145
https://doi.org/10.1002/bit.1145
Publikováno v:
Cytotechnology. 32:97-107
Hybridoma cells were grown at steady state under both reductiveand oxidative stress and the intracellular fluxes weredetermined by mass-balancing techniques. By decreasing the dissolved oxygen pressure (pO2) in the bioreactor, the reduced formof nico
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::67a2fdd4a86da5c9ed9af55b478e5943
https://doi.org/10.1023/a:1008142218103
https://doi.org/10.1023/a:1008142218103
Publikováno v:
Cytotechnology. 29:167-176
The analysis of metabolic fluxes of large stoichiometric systems is sensitive to measurement errors in metabolic uptake and production rates. It is therefore desirable to independently test the consistency of measurement data, which is possible if at
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bd2c58f97a969ef9ab3725383a5be18a
https://doi.org/10.1023/a:1008063324292
https://doi.org/10.1023/a:1008063324292
Publikováno v:
Biotechnology and Bioengineering, 58, 258-262
Biotechnology and Bioengineering 58 (1998)
Biotechnology and Bioengineering 58 (1998)
The estimation of intracellular fluxes of mammalian cells using only mass balances of the relevant metabolites is not possible because the set of linear equations defined by these mass balances is underdetermined. In order to quantify fluxes in cycli
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::498304ba8a9e53e96d911566c5856dab
https://doi.org/10.1002/(sici)1097-0290(19980420)58:2/3<258::aid-bit20>3.0.co
https://doi.org/10.1002/(sici)1097-0290(19980420)58:2/3<258::aid-bit20>3.0.co
Publikováno v:
Biotechnology and Bioengineering, 45, 524-535
Biotechnology and Bioengineering 45 (1995)
Biotechnology and Bioengineering 45 (1995)
The determination of the respiration quotient (RQ = CER/OUR) has not been used so far as a tool for understanding animal cell metabolism. This is due to problems in measuring the carbon dioxide evolution rate (CER) rather than the oxygen uptake rate
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0e9832304d036ee9ed43486e48718bd
https://doi.org/10.1002/bit.260450610
https://doi.org/10.1002/bit.260450610
Publikováno v:
Biotechnology and Bioengineering 57 (1998)
Biotechnology and Bioengineering, 57, 447-453
Biotechnology and Bioengineering, 57, 447-453
The effect of added ammonia on the intracellular fluxes in hybridoma cells was investigated by metabolic-flux balancing techniques. It was found that, in ammonia-stressed hybridoma cells, the glutamate-dehydrogenase flux is in the reverse direction c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98d5831f65452c5bffb349302ff898ee
https://research.wur.nl/en/publications/activity-of-glutamate-dehydrogenase-is-increased-in-ammonia-stres
https://research.wur.nl/en/publications/activity-of-glutamate-dehydrogenase-is-increased-in-ammonia-stres
Autor:
Bram Timmerarends, C. D. de Gooijer, Peter Skrabal, Elmar Heinzle, Georg Schmid, Johannes Tramper, Ahmet Ozemre, H.P.J. Bonarius
Publikováno v:
Animal Cell Technology ISBN: 9789401062732
The analysis of the intracellular metabolic fluxes in mammalian cells can be used for optimizing culture media, for tracing metabolic bottle-necks and for toxicological and metabolic studies. It is desirable to determine metabolic fluxes without usin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3d6d5c3d48b6ded5c0b73156078f4d7f
https://doi.org/10.1007/978-94-011-5404-8_99
https://doi.org/10.1007/978-94-011-5404-8_99
Publikováno v:
Animal cell technology: developments towards the 21st Century, E.C. Beuvery et al. (eds). Kluwer Acad. Publ., Dordrecht
Animal Cell Technology: Developments Towards the 21st Century ISBN: 9789401041959
Animal Cell Technology: Developments Towards the 21st Century ISBN: 9789401041959
Using a stoichiometric matrix it is possible to determine metabolic fluxes in animal cells if two conditions are fulfilled
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c9ddc1345eaa79db35bcff6d3a1f9b4
https://research.wur.nl/en/publications/determination-of-metabolic-fluxes-in-animal-cells
https://research.wur.nl/en/publications/determination-of-metabolic-fluxes-in-animal-cells
Publikováno v:
Animal Cell Technology: Developments Towards the 21st Century ISBN: 9789401041959
Animal cell technology: developments towards the 21st Century, E.C. Beuvery et al. (eds.). Kluwer Acad. Publ., Dordrecht
Animal cell technology: developments towards the 21st Century, E.C. Beuvery et al. (eds.). Kluwer Acad. Publ., Dordrecht
The determination of the respiration quotient (RQ = CER/OUR) has so far not been used as a tool for understanding animal cell metabolism. This has been due to problems in measuring the carbon dioxide evolution rate (CER) rather than the oxygen uptake
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1ead5cf5b92fecea8cfa03b26a1578e
https://doi.org/10.1007/978-94-011-0437-1_106
https://doi.org/10.1007/978-94-011-0437-1_106
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