Zobrazeno 1 - 10
of 65
pro vyhledávání: '"H.B. LéJohn"'
Publikováno v:
Journal of Biological Chemistry. 269:4523-4531
The gene for an NAD-specific glutamate dehydrogenase (NAD-GDH) that is allosterically activated by NADP+ (non-substrate) was cloned, and its physical structure and nucleotide sequence was determined. The gene consists of 9 introns and 10 exons; the 1
Publikováno v:
Journal of Biological Chemistry. 265:15245-15252
We have isolated and characterized by restriction endonuclease mapping, transcription pattern, and DNA sequencing a beta-tubulin gene from the coenocytic freshwater protoctist, Achlya klebsiana. The gene is intronless and has a single open reading fr
Autor:
H.B. LéJohn
Publikováno v:
Journal of Biological Chemistry. 264:19366-19372
I report on the isolation, structural analysis, and in vivo expression patterns of a fungal calmodulin gene. The gene is intronless and encodes a protein of 148 amino acid residues that is 92% homologous with vertebrate calmodulins. Through S1 nuclea
Publikováno v:
Journal of Biological Chemistry. 249:4016-4020
Achlya is a filamentous coenocytic fungus that grows only in the presence of Ca2+. The Ca2+ transport (uptake and binding) system of this organism was studied and shown to consist of two components: a low molecular weight Ca2+ binding glycopeptide pr
Autor:
H.B. LéJohn, Roselynn M. W. Stevenson
Publikováno v:
Canadian Journal of Microbiology. 28:1165-1172
i temperature dependent. Only tryptophan uptake was anomalous, for while its transport was inhibited, its binding to a cell wall - membrane proteoglycan was stimulated severalfold. From kinetic studies, we deduced that the analogues and tryptophan we
Publikováno v:
Journal of Biological Chemistry. 246:2116-2126
Lactate dehydrogenase (d-lactate:oxidoreductase, EC 1.1.1.28) from the homolactate-fermenting fungus, Pythium debaryanum, has been purified to a homogeneous-preparation with a yield of 10 to 12%. The enzyme has a molecular weight of approximately 98,
Autor:
Roselynn M. Stevenson, H.B. LéJohn
Publikováno v:
Journal of Biological Chemistry. 246:2127-2135
Purified NAD-specified glutamic dehydrogenases from two fungi, Pythium debaryanum and Achlya sp. (1969), have been studied kinetically, and some of their physical properties elucidated. The enzymes are regulated by a multitude of allosteric effectors
Autor:
Linda E. Cameron, H.B. LéJohn
Publikováno v:
Journal of Biological Chemistry. 247:4729-4739
When the transport of Ca++ and other bivalent cations was arrested in the filamentous coenocytic fungus Achlya the transport of amino acids and assimilation of these precursors into proteins was promptly terminated. Uptake of thymidine but not of ura
Autor:
H.B. LéJohn, Linda E. Cameron
Publikováno v:
Biochemical and Biophysical Research Communications. 54:1053-1060
A glycoprotein that binds about 20 atoms of Ca per mole has been purified from the osmotic shock fluid of some unicellular coenocytic water-molds, Achlya spp. and Blastocladiella emersonii . The binding of calcium is allosterically regulated by N6-(s
Autor:
Roselynn M. Stevenson, H.B. LéJohn
Publikováno v:
Journal of Biological Chemistry. 245:3890-3900
A novel form of allosteric control in which NAD-specific glutamic dehydrogenases obtained from taxonomically related fungi are activated by NADP+, NADPH, and P-enolpyruvate has been elucidated. Citrate and isocitrate are equally effective as inhibito