Zobrazeno 1 - 10
of 34
pro vyhledávání: '"H.-D. Hunger"'
Publikováno v:
Analytical Biochemistry. 186:159-164
A new system for high-sensitivity protein detection by an immunoenzymatic “contact-copy” procedure is described. It is based on two components: (i) a microbiologically produced bifunctional fusion protein of protein A and neomycin phosphotransfer
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b7b46579210141a92f1a889ba7c6bcd1
https://doi.org/10.1016/0003-2697(90)90590-6
https://doi.org/10.1016/0003-2697(90)90590-6
Publikováno v:
Analytical biochemistry. 217(1)
This report describes a method for simultaneous quantitative determination of antigenes from protein blots based on the highly sensitive "contact-copy" procedure, applying the phosphorylation reaction as a general detection principle. Using ultrasens
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bdc2a5aedc5e26de61ba8c16a4cf8a65
https://pubmed.ncbi.nlm.nih.gov/8203743
https://pubmed.ncbi.nlm.nih.gov/8203743
Publikováno v:
Analytical biochemistry. 187(1)
A new sensitive method for antigen detection employing a phosphorylation reaction is described using human serum albumin as a model. The antigen is initially bound to the surface of polystyrene microtiter plates and reacted with an antibody (rabbit).
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52c3ab9f0260006138e809c2425deb37
https://pubmed.ncbi.nlm.nih.gov/2164793
https://pubmed.ncbi.nlm.nih.gov/2164793
Autor:
H.-D. Hunger, Ch. Coutelle, G. Behrendt, Chr. Flachmeier, A. Rosenthal, A. Speer, H. Breter, R. Szargan, P. Franke, J. Stahl, N.V. Cuong, G. Barchend
Publikováno v:
Analytical Biochemistry. 156:286-299
A novel two-dimensional cyanuric chloride-activated (CCA) paper has been developed. It is composed of a cellulosic base, covalently bound cyanuric chloride, and microprecipitated complex cyanuric chloride-sodium chloride crystals on its surface. CCA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f57dd746be400cafabaf81ac23df5002
https://doi.org/10.1016/0003-2697(86)90255-1
https://doi.org/10.1016/0003-2697(86)90255-1
Publikováno v:
Analytical Biochemistry. 165:45-55
Conditions for the optimal use of cyanuric chloride-activated (CCA) paper in Southern transfer hybridization experiments of genomic DNA were investigated. They depend critically on pH and ionic strength during transfer and on the composition of the h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7eda9f42992274b59afeb9fad1399cce
https://doi.org/10.1016/0003-2697(87)90199-0
https://doi.org/10.1016/0003-2697(87)90199-0
Publikováno v:
Acta Biotechnologica. 6:34-38
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::71017f83508f1d11d5a11cb7ce91a6d9
https://doi.org/10.1002/abio.370060120
https://doi.org/10.1002/abio.370060120
Publikováno v:
Padiatrie und Grenzgebiete. 28(6)
The introduction of molecular human genetics has become a paradigma for the application of genetic engineering in medicine. The main principles of this technology are the isolation of molecular probes, their application in hybridization reactions, sp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::431ce2313e1f9547cd4ee43629f40275
https://pubmed.ncbi.nlm.nih.gov/2697834
https://pubmed.ncbi.nlm.nih.gov/2697834
Publikováno v:
Journal of biochemical and biophysical methods. 18(4)
A new general method for the determination of neomycin phosphotransferase (NPT) II (EC 2.7.1.95) activity in cell extracts after separation in SDS-polyacrylamide gels is described. The enzymatic activity of NPT II is restored after SDS-polyacrylamide
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b21ced383e6f25db89b2fd0d3a4dddd3
https://pubmed.ncbi.nlm.nih.gov/2550536
https://pubmed.ncbi.nlm.nih.gov/2550536
Publikováno v:
Biochimica et biophysica acta. 653(3)
A procedure for the binding of nucleic acids to cyanuric chloride-activated paper is described. The pH value of the binding buffer has a substantial influence on the binding reaction and on the stability of binding. Substantial and stable binding of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::7b76b188dfc58b3335eb6a36ade03ace
https://pubmed.ncbi.nlm.nih.gov/6166322
https://pubmed.ncbi.nlm.nih.gov/6166322
Publikováno v:
Acta biologica et medica Germanica. 36(3-4)
Globin mRNA isolated from rabbit reticulocytes showed an unusually high molecular weight for the beta-chain mRNA (280000 daltons), a large difference between the molecular weight of the alpha-chain mRNA (230000 daltons) and the beta-chain mRNA and a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e9fa5240a20f5db91c4cbd4e481f7d4d
https://pubmed.ncbi.nlm.nih.gov/596057
https://pubmed.ncbi.nlm.nih.gov/596057