Zobrazeno 1 - 10
of 115
pro vyhledávání: '"H. U. Koop"'
Publikováno v:
Molecular Genetics and Genomics. 269:340-349
Regulation of chloroplast gene expression involves networked and concerted interactions of nucleus-encoded factors with their target sites on untranslated regions (UTRs) of chloroplast transcripts. So far, only a few cis -acting elements within such
Publikováno v:
Scandinavian Journal of Forest Research. 15:151-160
A particle inflow gun enabled efficient production of transgenic plantlets of Picea abies from embryogenic suspension cultures. In transient assays, the Zea ubiquitin promoter was 12-16 times as active as the 35S promoter. For stable transformation,
Autor:
H. U. Koop, A. Mehra-Palta, S. Goes, G. Nagy, E.-M. Troidl, S. Tyagi, Reinhold G. Herrmann, Waltraud Kofer
Publikováno v:
Plant Cell Reports. 17:605-611
A favourable combination of genetic features in the genus Oenothera offers access to fundamental biological aspects that are not readily approached with other materials. We have developed protocols for cell and tissue culture as well as for transform
Publikováno v:
Plant Cell Reports. 17:601-604
A protocol is presented for regenerating plants from leaf protoplasts of Oenothera. The method uses (1) embedding of isolated protoplasts at high cell densities in thin alginate layers, (2) initial culture in B5 medium containing 3 mg l–1 α-naphth
Publikováno v:
Molecular and General Genetics MGG. 258:166-173
Plastids contain a NAD(P)H-plastoquinone-oxidoreductase (NDH complex) which is homologous to the eubacterial and mitochondrial NADH-ubiquinone-oxidoreductase (complex I), but the metabolic function of the enzyme is unknown. The enzyme consists of at
Publikováno v:
Protoplasma. 204:114-118
Regeneration of plants from protoplasts is regarded a difficult and lengthy procedure which requires well developed skills on the side of the experimenter. Therefore, where alternative procedures for genetic engineering of plants are available, proto
Publikováno v:
Protoplasma. 169:89-96
Zygotic embryos ofArabidopsis thaliana showed three different types of developmental response, when cultured in vitro: (1) normal development, (2) formation of morphogenetic callus, and (3) somatic embryogenesis. Early zygotic embryos were mechanical
Autor:
H. U. Koop, B. Spörlein
Publikováno v:
Theoretical and Applied Genetics. 83:1-5
It has recently been shown that lipofectin, a commercially available preparation of cationic liposomes is capable of animal and plant cell line transfection. Here, it is analyzed with respect to its toxicity for higher plant protoplasts and used for
Publikováno v:
Theoretical and Applied Genetics. 82:717-722
Evidence is presented for the introduction of functional copies of the GUS-reporter gene with plastid regulatory signals into chloroplasts after treatment of Nicotiana plumbaginifolia leaf protoplasts with PEG. GUS-activity is found in cells derived
Autor:
H. U. Koop, Mechthild Bolik
Publikováno v:
Protoplasma. 162:61-68
In order to identify microspores, suitable for transformation via microinjection of DNA, single microspores of barley (Hordeum vulgare L.) were selected after initial preculture of anthers floating on liquid media and analysed for their development i