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Akademický článek

Boosting genome editing efficiency in human cells and plants with novel LbCas12a variants

Autor: Liyang Zhang, Gen Li, Yingxiao Zhang, Yanhao Cheng, Nathaniel Roberts, Steve E. Glenn, Diane DeZwaan-McCabe, H. Tomas Rube, Jeff Manthey, Gary Coleman, Christopher A. Vakulskas, Yiping Qi

Publikováno v: Genome Biology, Vol 24, Iss 1, Pp 1-19 (2023)

Abstract Background Cas12a (formerly known as Cpf1), the class II type V CRISPR nuclease, has been widely used for genome editing in mammalian cells and plants due to its distinct characteristics from Cas9. Despite being one of the most robust Cas12a

Externí odkaz: https://doaj.org/article/db0922eabb8c46788d5ef6a754bef15a

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Akademický článek

Transcription factor paralogs orchestrate alternative gene regulatory networks by context-dependent cooperation with multiple cofactors

Autor: Siqian Feng, Chaitanya Rastogi, Ryan Loker, William J. Glassford, H. Tomas Rube, Harmen J. Bussemaker, Richard S. Mann

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-19 (2022)

Feng et al. compare the genome-wide binding and activity of two Drosophila Hox transcription factors that determine the identities of different fly legs. For binding events that are Hox specific, two cofactors, Exd and Dll, are used to achieve specif

Externí odkaz: https://doaj.org/article/1d593ab08c24437783d53d88f829132d

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Akademický článek

AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-15 (2021)

The utility of AsCas12a can be limited to poor editing efficiency. Here the authors identify a variant, “AsCas12a Ultra”, that has high on-target specificity demonstrated through editing of clinically relevant T cell genes.

Externí odkaz: https://doaj.org/article/0168163683a24415a8a5f32e5a19e09a

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Akademický článek

Author Correction: AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-1 (2021)

Externí odkaz: https://doaj.org/article/8e393b3b2eaf4c56989cd44fec2d1138

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Akademický článek

Sequence features accurately predict genome-wide MeCP2 binding in vivo

Autor: H. Tomas Rube, Wooje Lee, Miroslav Hejna, Huaiyang Chen, Dag H. Yasui, John F. Hess, Janine M. LaSalle, Jun S. Song, Qizhi Gong

Publikováno v: Nature Communications, Vol 7, Iss 1, Pp 1-12 (2016)

MeCP2 is critical for proper brain development, and mutations in the gene encoding MeCP2 are responsible for several neurological disorders. Here, the authors show that the previously reported genome-wide preference of MeCP2 to methylated CpGs is in

Externí odkaz: https://doaj.org/article/1be597bf744b49939e33398defbd03c2

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Akademický článek

Effective functionalization of carbon nanotubes for bisphenol F epoxy matrix composites

Autor: Zhe Wang, Henry A. Colorad, Zhan-Hu. Guo, Hansang. Kim, Cho-Long Park, H. Tomas Hahn, Sang-Gi. Lee, Kun-Hong Lee, Yu-Qin Shang

Publikováno v: Materials Research, Vol 15, Iss 4, Pp 510-516 (2012)

A brand-new type of multifunctional nanocomposites with high DC conductivity and enhanced mechanical strength was fabricated. Ionic liquid functionalized Carbon Nanotubes (CNTs-IL) were embedded into epoxy matrix with covalent bonding by the attached

Externí odkaz: https://doaj.org/article/d19e5c45e4dd427f94b967b4742a946e

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