Zobrazeno 1 - 10
of 49
pro vyhledávání: '"H. Spiele"'
Autor:
J. H. M. Temmink, H. Spiele
Publikováno v:
The Journal of Cell Biology
Publikováno v:
Journal of Cell Science. 88:313-325
The interaction of H-2 antigens and plasma membrane-associated filaments was studied on dry-cleaved preparations of immunogold-labelled lymphoma cells. In prefixed cells, the plasma membrane-associated network was isotropic without any prevailing dir
Publikováno v:
The Journal of Cell Biology
To visualize the localization of cell surface constituents in relation to the plasma membrane-associated filament network, we developed a method based on a combination of immunogold labeling and dry-cleaving. For labeling we used trinitrophenyl-deriv
Autor:
D A, Mesland, H, Spiele
Publikováno v:
Journal of Cell Science. 68:113-137
In cultured H35 hepatoma cells membrane-associated cortical networks have a microtrabecular appearance as revealed by dry-cleaving. Filaments having diameters of 15 nm can be readily distinguished within these networks and have not been described pre
Publikováno v:
The Journal of Immunology. 104:1406-1416
Hemoglobin-free membranes and erythrocytes of group O, after reaction with ferritin-labeled rabbit antibody to purified virus receptor substance, were examined by electron microscopy. Antibody was found to bind specifically to discrete sites, in thin
Publikováno v:
Experimental cell research. 132(1)
Dry-cleaving is introduced as a new technique for visualization of the cytoplasmic side of adherent membrane in cultured cells. Basically, cells are fixed and critical point-dried in situ and subsequently broken by means of adhesive tape. The plane o
Autor:
J.H. Temmink, H. Spiele
Publikováno v:
Journal of cell science. 41
The morphology of the cytoskeleton of normal 3T3 fibroblasts was studied in the electron microscope on embedded and sectioned cells as well as on critical-point-dried whole mounts. Four ultrastructurally different cytoplasmic domains could be disting
Autor:
H. Spiele, J.H.M. Temmink
Publikováno v:
Cell biology international reports. 2(1)
Attached murine fibroblasts were subjected to different fixation and dehydration procedures in order to study the preservation of cytoskeletal elements. The organization of the microtubular and microfilamentous system was identical whether or not pos
Autor:
C A, Feltkamp, T M, Feltkamp-Vroom, J, Koudstaal, P, van Heerds, H, Spiele, C B, de Graaf-Reitsma
Publikováno v:
Advances in experimental medicine and biology. 114
Autor:
J.H. Temmink, H. Spiele
Publikováno v:
Journal of cell science. 48
Normal 3T3 cells were exposed to vinblastine and cytochalasin B in an atttempt to correlate the morphological changes of the cell surface as seen in the scanning electron microscope with ultrastructural changes of the cytoskeletal elements as seen in