Zobrazeno 1 - 10
of 16
pro vyhledávání: '"H. P. Zassenhaus"'
Publikováno v:
The Scientific World Journal, Vol 1, Pp 55-55 (2001)
Externí odkaz:
https://doaj.org/article/9b9f5f5d11f64b27b0c0304121e83938
Publikováno v:
The Scientific World Journal, Vol 1, Pp 59-59 (2001)
Externí odkaz:
https://doaj.org/article/41f467a99f4c4bf4be11d80ee7810910
Publikováno v:
Diabetes, obesitymetabolism. 9
Low levels of somatic mutations accumulate in mitochondrial DNA (mtDNA) as we age; however, the pathogenic nature of these mutations is unknown. In contrast, mutational loads of30% of mtDNA are associated with electron transport chain defects that re
Publikováno v:
The Journal of biological chemistry. 268(10)
RNA turnover in eukaryotes is thought to require 3'-exonuclease activity but so far no RNase with that specificity has been isolated from a eukaryote. We report here on the purification and characterization of a 3'-exoribonuclease isolated from the m
Autor:
J, Min, H P, Zassenhaus
Publikováno v:
BioTechniques. 13(6)
We describe a procedure to quantify specific, radioactively labeled RNA sequences. This procedure combines hybrid selection of an RNA using biotinylated oligonucleotides with gel electrophoretic analysis of the selected RNA. We show that the hybrid s
Autor:
J J, Min, H P, Zassenhaus
Publikováno v:
SAAS bulletin, biochemistry and biotechnology. 4
We have purified and characterized a novel exoribonuclease that was isolated from the mitochondria of Saccharomyces cerevisiae. The enzyme degraded RNA in a 3' to 5' direction and was dependent on nucleotide triphosphates for activity. All eight of t
Autor:
R A Butow, H P Zassenhaus
Publikováno v:
Journal of Biological Chemistry. 259:8417-8421
We have determined whether two 46-base pair (bp) GC-rich clusters present in the coding region of the yeast mitochondrial var 1 gene are transcribed and retained in the putative mRNA for the var 1 protein. One GC cluster is found in all var 1 alleles
Publikováno v:
Journal of Biological Chemistry. 259:6019-6027
Transcription of the yeast mitochondrial var 1 gene encoding the mitochondrial small ribosomal subunit protein, var 1, has been examined in wild type and petite strains by Northern hybridization, S 1 nuclease protection experiments, and analysis of p
Autor:
Philip S. Perlman, H. P. Zassenhaus
Publikováno v:
Current Genetics. 6:179-188
Several mit mutants mapping within or near the var1 determinant region have been characterized genetically and biochemically. These mutants were isolated using a new enrichment protocol which simplifies the isolation and identification of rare respir
Publikováno v:
Journal of Biological Chemistry. 257:6581-6587
We have identified transcripts with sequence homology to the var1 region on yeast mtDNA. In wild type, and in cytoplasmic petite strains retaining the var1 region, we detect four RNA species, 19 S, 16 S, 14 S, and 13 S, which hybridize to var1 DNA pr