Výsledky vyhledávání

Turn-on Fluorescent Biosensors for Imaging Hypoxia-like Conditions in Living Cells

Autor: Santiago Guisán-Ceinos, Alexandra R. Rivero, Fernando Romeo-Gella, Silvia Simón-Fuente, Silvia Gómez-Pastor, Natalia Calvo, Alejandro H. Orrego, José Manuel Guisán, Inés Corral, Francisco Sanz-Rodriguez, Maria Ribagorda

Publikováno v: Biblos-e Archivo. Repositorio Institucional de la UAM
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We present the synthesis, photophysical properties, and biological application of nontoxic 3-azo-conjugated BODIPY dyes as masked fluorescent biosensors of hypoxia-like conditions. The synthetic methodology is based on an operationally simple N=N bon

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce1424c580493e730d6b63b292244f11
https://pubmed.ncbi.nlm.nih.gov/35486830

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Ultrahigh-Throughput Screening of Metagenomic Libraries Using Droplet Microfluidics

Autor: Mercedes Sánchez-Costa, Davide Agostino Cecchini, Aurelio Hidalgo, Jesús Fernández-Lucas, Alejandro H. Orrego

Publikováno v: Methods in Molecular Biology ISBN: 9781071618257

Droplet microfluidics enables the ultrahigh-throughput screening of the natural or man-made genetic diversity for industrial enzymes, with reduced reagent consumption and lower costs than conventional robotic alternatives. Here we describe an example

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5b5c13a8f46a4a20604bff782497f90f
https://doi.org/10.1007/978-1-0716-1826-4_2

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Ultrahigh-Throughput Screening of Metagenomic Libraries Using Droplet Microfluidics

Autor: Davide Agostino, Cecchini, Mercedes, Sánchez-Costa, Alejandro H, Orrego, Jesús, Fernández-Lucas, Aurelio, Hidalgo

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2397

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::8be8d15e130a5f7f3636b89a6f5c37f2
https://pubmed.ncbi.nlm.nih.gov/34813057

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Self-sufficient asymmetric reduction of β-ketoesters catalysed by a novel and robust thermophilic alcohol dehydrogenase coimmobilised with NADH

Autor: José Berenguer, Alejandro H. Orrego, Daniel Andrés-Sanz, Fernando López-Gallego, Jose M. Guisan, Mercedes Sánchez-Costa, Javier Rocha-Martín, Susana Velasco-Lozano

Publikováno v: Biblos-e Archivo. Repositorio Institucional de la UAM
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Catalysis Science & Technology

β-Hydroxyesters are essential building blocks utilised by the pharmaceutical and food industries in the synthesis of functional products. Beyond the conventional production methods based on chemical catalysis or whole-cell synthesis, the asymmetric

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e17285a3db597403f77b87df956ebd1
https://doi.org/10.5281/zenodo.4697225

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Functionalization of Porous Cellulose with Glyoxyl Groups as a Carrier for Enzyme Immobilization and Stabilization

Autor: José M. Fraile, Javier Rocha-Martín, Jose M. Guisan, Fernando López-Gallego, Alejandro H. Orrego, Sonia Moreno-Pérez, Sandro Martins de Oliveira, Susana Velasco-Lozano

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
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The functionalization of the internal surface of macroporous carriers with glyoxyl groups has proven to highly stabilize a large variety of enzymes through multipoint covalent immobilization. In this work, we have translated the surface chemistry dev

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b47b3341797b710c46b653add15085fe
https://pubmed.ncbi.nlm.nih.gov/33423456

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High Stabilization of Enzymes Immobilized on Rigid Hydrophobic Glyoxyl-Supports: Generation of Hydrophilic Environments on Support Surfaces

Autor: Alejandro H. Orrego, Javier Rocha-Martín, Jose M. Guisan, Justo Pedroche, María del Carmen Millán-Linares, Maria Romero-Fernández

Publikováno v: Catalysts, Vol 10, Iss 676, p 676 (2020)
Catalysts
Volume 10
Issue 6
Digital.CSIC. Repositorio Institucional del CSIC
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© 2020 by the authors.
Very rigid supports are useful for enzyme immobilization to design continuous flow reactors and/or to work in non-conventional media. Among them, epoxy-methacrylic supports are easily functionalized with glyoxyl groups, w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b755de12a8703d6df656dad2586aa7fe
https://www.mdpi.com/2073-4344/10/6/676

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Coimmobilization and colocalization of a glycosyltransferase and a sucrose synthase greatly improves the recycling of UDP-glucose: Glycosylation of resveratrol 3-O-β-D-glucoside

Autor: Jose M. Guisan, Lara Trobo-Maseda, Javier Rocha-Martín, Alejandro H. Orrego

Publikováno v: International journal of biological macromolecules. 157

Glycosylation is one of the most efficient biocompatible methodologies to enhance the water solubility of natural products, and therefore their bioavailability. The excellent regio- and stereoselectivity of nucleotide sugar-dependent glycosyltransfer

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f0b92fa08d40273ddb94673731cee30
https://pubmed.ncbi.nlm.nih.gov/32344088

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Stabilization of Glycosylated β-Glucosidase by Intramolecular Crosslinking Between Oxidized Glycosidic Chains and Lysine Residues

Autor: Gloria Fernández-Lorente, Paulo Waldir Tardioli, Jose M. Guisan, Benevides C. C. Pessela, Cristiane S. Farinas, Alejandro H. Orrego, Laura Marina Pinotti

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
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Many industrial enzymes can be highly glycosylated, including the β-glucosidase enzymes. Although glycosylation plays an important role in many biological processes, such chains can cause problems in the multipoint immobilization techniques of the e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8659a52b972da07a53d0583d7cc5bed
https://pubmed.ncbi.nlm.nih.gov/32382943

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