Výsledky vyhledávání - "H. David Fischer"

Affinity purification of a chimeric nicotinic acetylcholine receptor in the agonist and antagonist bound states

Autor: Mark L. Wolfe, Jacob Bode, H. David Fischer, Merrill S. Babcock, W. Rodney Mathews, Gurnam S. Gill, Robert L. Garlick, Eric T. Baldwin, Shenping Liu, Jeanne S. Chang, Bruce N. Rogers, Brandon J. Margolis, Eric T. Lund, Vince Groppi

Publikováno v: Protein Expression and Purification. 79:102-110

Nicotinic acetylcholine receptors (nAChRs) form ligand-gated ion channels that mediate fast signal transmission at synapses. These receptors are members of a large family of pentameric ion channels that are of active medical interest. An expression s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf5ad9a679c3ecb882f12cae4e556bb3
https://doi.org/10.1016/j.pep.2011.05.019

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Structural and Thermodynamic Characterization of the TYK2 and JAK3 Kinase Domains in Complex with CP-690550 and CMP-6

Publikováno v: Journal of Molecular Biology. 400:413-433

Janus kinases (JAKs) are critical regulators of cytokine pathways and attractive targets of therapeutic value in both inflammatory and myeloproliferative diseases. Although the crystal structures of active JAK1 and JAK2 kinase domains have been repor

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b19fab0c9dc63112be2b1f9f66fc065
https://doi.org/10.1016/j.jmb.2010.05.020

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Expression, purification, and characterization of TYK-2 kinase domain, a member of the Janus kinase family

Autor: Thomas L. Emmons, Alfredo G. Tomasselli, Robin A. Weinberg, H. David Fischer, Jill Chrencik, Brian Korniski, Jacqueline E. Day, Stacy Brown, Joseph W. Leone, Ann D. Wrightstone, Cynthia D. Sommers, Jeffrey L. Hirsch, Arthur J. Wittwer, Troii Hall, Jennifer A. Gormley

Publikováno v: Biochemical and Biophysical Research Communications. 396:543-548

The Janus kinase family consists of four members: JAK-1, -2, -3 and TYK-2. While JAK-2 and JAK-3 have been well characterized biochemically, there is little data on TYK-2. Recent work suggests that TYK-2 may play a critical role in the development of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4568dae04b1e101103ca4b31d2d17487
https://doi.org/10.1016/j.bbrc.2010.04.141

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Expression, purification, characterization and crystallization of non- and phosphorylated states of JAK2 and JAK3 kinase domain

Autor: Thomas L. Emmons, James R. Kiefer, Joseph W. Leone, John F. Schindler, Melissa S. Harris, Jacqueline E. Day, Siradanahalli Guru, Alfredo G. Tomasselli, Robin A. Weinberg, Sandra Lightle, H. David Fischer, Jeffrey L. Hirsch, Jill Chrencik, Jennifer A. Gormley, Jennifer M. Williams, Matthew J. Saabye, Troii Hall

Publikováno v: Protein Expression and Purification. 69:54-63

Janus-associated kinases (JAKs) play critical roles in cytokine signaling, and have emerged as viable therapeutic targets in inflammation and oncology related diseases. To date, targeting JAK proteins with highly selective inhibitor compounds have re

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68adb1eba9066345bc1000db1eef31a2
https://doi.org/10.1016/j.pep.2009.09.011

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Purification and characterization of recombinant human soluble guanylate cyclase produced from baculovirus-infected insect cells

Autor: Beverly A. Reitz, Joseph W. Leone, Michael J. Bienkowski, Daniel F. Curran, Karl J. Mathis, Thomas L. Emmons, Jacqueline E. Day, Mary E. Shuck, Mark C. Walker, Alfredo G. Tomasselli, H. David Fischer

Publikováno v: Protein Expression and Purification. 65:133-139

Soluble guanylate cyclase (sGC) has been purified from 100 L cell culture infected by baculovirus using the newer and highly effective titerless infected-cells preservation and scale-up (TIPS) method. Successive passage of the enzyme through DEAE, Ni

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::429666b5c5f068867bf311b4dcaba1ae
https://doi.org/10.1016/j.pep.2009.01.001

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The titerless infected-cells preservation and scale-up (TIPS) method for large-scale production of NO-sensitive human soluble guanylate cyclase (sGC) from insect cells infected with recombinant baculovirus

Autor: Beverly A. Reitz, Thomas L. Emmons, Alfredo G. Tomasselli, H. David Fischer, Michael J. Bienkowski, S. Edward Lee, David Wasilko, Karl J. Mathis, Kim Jonelle Stutzman-Engwall

Publikováno v: Protein Expression and Purification. 65:122-132

Compounds capable of stimulating soluble guanylate cyclase (sGC) activity might become important new tools to treat hypertension. While rational design of these drugs would be aided by elucidation of the sGC three-dimensional structure and molecular

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d67ccc292e9fe1d6be21cbe94fae775
https://doi.org/10.1016/j.pep.2009.01.002

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Differential kinetics and inhibition of purified recombinant tyrosine kinase 2 (TYK-2) and its catalytic domain JH-1

Autor: Alfredo G. Tomasselli, Robin A. Weinberg, H. David Fischer, Lyle E. Pegg, Ann D. Wrightstone, Cynthia D. Sommers, Stacy Brown, Thomas L. Emmons, Arthur J. Wittwer, Eric T. Baima, Jeffrey L. Hirsch

Publikováno v: Protein and peptide letters. 19(5)

The Janus kinase (JAK) family consists of four members: JAK-1, -2, -3 and tyrosine kinase 2 (TYK-2). Recent work suggests that cytokine signaling through TYK-2 may play a critical role in a number of inflammatory processes. We recently described the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fe4a1b316bfa5b6ee01547811330368
https://pubmed.ncbi.nlm.nih.gov/22486643

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Biochemical diversity in a phage display library of random decapeptides

Autor: H. David Fischer, John E. Mott, Michael E. Degraaf, Robert M. Miceli

Publikováno v: Gene. 128:13-17

Detailed knowledge and understanding of the structure and function of biologically important macromolecules is frequently insufficient to permit rational, de novo design of recognition molecules and therapeutics. Traditional drug discovery has, thus,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da6bca9bff4bbcf048ff679c45e15bf3
https://doi.org/10.1016/0378-1119(93)90147-u

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ADAM8 substrate specificity: influence of pH on pre-processing and proteoglycan degradation

Autor: Troii Hall, Marc D. Zack, Lyle E. Pegg, Alfredo G. Tomasselli, H. David Fischer, Adele M. Pauley

Publikováno v: Archives of biochemistry and biophysics. 491(1-2)

A disintegrin and metalloprotease-8 (ADAM8) is thought to play a role in cancer and inflammatory diseases such as allergy, arthritis, and asthma. Despite the implication of ADAM8 in these diseases, the functional role of ADAM8 catalytic activity rema

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9cb533b0451eaba3dde7368978a75863
https://pubmed.ncbi.nlm.nih.gov/19766586

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A peptide inhibitor of cholesteryl ester transfer protein identified by screening a bacteriophage display library

Autor: H. David Fischer, Laurence A. Erickson, Paul D. Bonin, Carol A. Bannow, Clark W. Smith

Publikováno v: The journal of peptide research : official journal of the American Peptide Society. 51(3)

We screened a bacteriophage display library of random decapeptides to identify peptide inhibitors of cholesteryl ester transfer protein (CETP). After affinity selection against CETP, bacteriophage-infected Escherichia coli were plated at clonal densi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::283467b35496b5bae5fc2c2861433f3f
https://pubmed.ncbi.nlm.nih.gov/9531425

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