Zobrazeno 1 - 10
of 51
pro vyhledávání: '"H. C. Birnboim"'
Autor:
H. C. Birnboim, Olle M. de Bruin
Publikováno v:
BMC Microbiology
Background DNA-based testing is becoming the preferred method both for identifying microorganisms and for characterizing microbial communities. However, no single DNA extraction method exists that is suitable for all types of microorganisms because b
Publikováno v:
British Journal of Cancer
Thymidylate synthase (TS) is the enzyme that catalyses the last step in de novo thymidylate synthesis. It is of interest clinically because it is an effective target for drugs such as 5-fluorouracil, often used in combination therapy. Despite a numbe
Autor:
H C Birnboim, Randy T. Cowling
Publikováno v:
Journal of Leukocyte Biology. 67:240-248
Stimulation of human peripheral blood granulocytes with the proinflammatory cytokine, granulocyte-macrophage colony-stimulating factor (GM-CSF), increases incorporation of [3H]uridine into RNA. We investigated the nature of the RNA synthesized under
Publikováno v:
Journal of Histochemistry & Cytochemistry. 47:1563-1573
Measurement of thymidylate synthase (hTS) using immunohistochemical techniques has been reported in several clinical studies. However, its value as a prognostic indicator is still not clear. To pursue this, we have developed a new rabbit polyclonal a
Publikováno v:
Arthritis & Rheumatism. 41:1772-1782
Objective To investigate the frequency and characteristics of hprt- mutant T lymphocytes in the peripheral blood and synovium of rheumatoid arthritis (RA) patients compared with controls, and to correlate these findings with disease parameters. Metho
Autor:
H. C. Birnboim, Steffany A. L. Bennett
Publikováno v:
Molecular Carcinogenesis. 20:366-375
Chronic inflammation is a recognized risk factor for human cancer, but the causal mechanisms are poorly understood. We previously demonstrated that platelet activating factor (PAF) can induce alterations in the in vitro growth properties of primary r
Autor:
J K Sandhu, H C Birnboim
Publikováno v:
Journal of Cellular Biochemistry. 66:219-228
Phorbol ester treatment of granulocytes triggers release of superoxide (O2.-) and a concomitant burst of DNA strand breaks. The relationship between the amount of O2.- and the number of DNA breaks has not previously been explored. To quantify the rel
Publikováno v:
Journal of Virological Methods. 50:245-255
Although a useful and important method of gene transfer, retroviral vectors can be genetically unstable. In the course of experiments using DOEJS, a retroviral vector able to confer expression of a H-ras oncogene and a neomycin resistance gene (neo)
Autor:
H C Birnboim, Randy T. Cowling
Publikováno v:
Journal of Biological Chemistry. 269:9480-9485
When human peripheral blood granulocytes were metabolically labeled at 37 degrees C with [32P]orthophosphate, inorganic polyphosphates became preferentially radiolabeled. Incorporation of radiolabel into the polymer appeared to be ATP-independent. [3
Publikováno v:
Free Radical Research. 20:113-117
8-Hydroxydeoxyguanosine (8-OHdG) is now widely used as a sensitive marker of oxidative damage to DNA. When human granulocytes are stimulated with TPA, they release a large quantity of reactive oxygen species (superoxide, hydrogen peroxide) which migh