Zobrazeno 1 - 10
of 252
pro vyhledávání: '"H. Brodsky"'
Autor:
Katharina E. Meijboom, Abbas Abdallah, Nicholas P. Fordham, Hiroko Nagase, Tomás Rodriguez, Carolyn Kraus, Tania F. Gendron, Gopinath Krishnan, Rustam Esanov, Nadja S. Andrade, Matthew J. Rybin, Melina Ramic, Zachary D. Stephens, Alireza Edraki, Meghan T. Blackwood, Aydan Kahriman, Nils Henninger, Jean-Pierre A. Kocher, Michael Benatar, Michael H. Brodsky, Leonard Petrucelli, Fen-Biao Gao, Erik J. Sontheimer, Robert H. Brown, Zane Zeier, Christian Mueller
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-17 (2022)
A hexanucleotide repeat expansion in C9ORF72 is the most common genetic cause of ALS and FTD. Here, the authors demonstrate CRISPR/Cas9 excision of the expansion results in a rescue of disease mechanisms in vivo and in vitro.
Externí odkaz:
https://doaj.org/article/3c12d94acb99423da250fefe20d4cde2
Autor:
Aamir Mir, Julia F. Alterman, Matthew R. Hassler, Alexandre J. Debacker, Edward Hudgens, Dimas Echeverria, Michael H. Brodsky, Anastasia Khvorova, Jonathan K. Watts, Erik J. Sontheimer
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
Resistance of gRNA to ubiquitous ribonucleases is required for CRISPR-Cas9-based therapeutics. Here, the authors explore chemical modifications at all positions of the crRNA guide and tracrRNA cofactor, and identify modified versions that are more po
Externí odkaz:
https://doaj.org/article/b945b146d35544ef9d5a4a30d633c3c0
Autor:
Janice A. Dominov, Laura A. Madigan, Joshua P. Whitt, Katerina L. Rademacher, Kristin M. Webster, Hesheng Zhang, Haruhiko Banno, Siqi Tang, Yifan Zhang, Nicholas Wightman, Emma M. Shychuck, John Page, Alexandra Weiss, Karen Kelly, Alper Kucukural, Michael H. Brodsky, Alexander Jaworski, Justin R. Fallon, Diane Lipscombe, Robert H. Brown
Publikováno v:
bioRxiv
Amyotrophic lateral sclerosis (ALS) is a severe neurodegenerative disorder affecting brain and spinal cord motor neurons. Mutations in the copper/zinc superoxide dismutase gene (SOD1) are associated with ∼20% of inherited and 1-2% of sporadic ALS c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0de160fc2e91574d01f7d30c4af0729
https://doi.org/10.1101/2023.05.05.539444
https://doi.org/10.1101/2023.05.05.539444
Publikováno v:
G3: Genes, Genomes, Genetics, Vol 6, Iss 10, Pp 3197-3206 (2016)
Adoption of a streamlined version of the bacterial clustered regular interspersed short palindromic repeat (CRISPR)/Cas9 defense system has accelerated targeted genome engineering. The Streptococcus pyogenes Cas9 protein, directed by a simplified, CR
Externí odkaz:
https://doaj.org/article/ca144083ff2a422cb941669ca1370127
Autor:
Ciearra B. Smith, Vincent van der Vinne, Eleanor McCartney, Adam C. Stowie, Tanya L. Leise, Blanca Martin-Burgos, Penny C. Molyneux, Lauren A. Garbutt, Michael H. Brodsky, Alec J. Davidson, Mary E. Harrington, Robert Dallmann, David R. Weaver
Publikováno v:
Journal of Biological Rhythms. 37:53-77
Circadian rhythms are endogenously generated physiological and molecular rhythms with a cycle length of about 24 h. Bioluminescent reporters have been exceptionally useful for studying circadian rhythms in numerous species. Here, we report developmen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::955a581ad73e472b0a85345c2176fdb9
https://doi.org/10.1177/07487304211069452
https://doi.org/10.1177/07487304211069452
Autor:
Ellen Sapp, Rachael Miller, Kathryn Chase, Sarah R. Oikemus, Eric Mick, Lihua Julie Zhu, Michael H. Brodsky, Miguel Sena-Esteves, Edward Hudgens, Akanksh Chaudhary, Lori A. Kennington, Scot A. Wolfe, Neil Aronin, Marian DiFiglia, Edith L. Pfister
Publikováno v:
Hum Gene Ther
Huntington's disease (HD) is a devastating, autosomal dominant neurodegenerative disease caused by a trinucleotide repeat expansion in the huntingtin (HTT) gene. Inactivation of the mutant allele by clustered regularly interspaced short palindromic r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e7171462802e83152be7b932753d6dc
https://doi.org/10.1089/hum.2020.323
https://doi.org/10.1089/hum.2020.323
Autor:
Maria Cristina Fernandez, Edan Hosking, Wesley Thompson, Brooke Roman, Robert Donofrio, Karen Cooper, Susanne Hinkley, Benjamin Bastin, Susan Alles, Mark Mozola, Michael H. Brodsky, Yi Chen, Danielle Keys
Publikováno v:
Journal of AOAC INTERNATIONAL. 103:523-532
Background: NeoSeekTM STEC is a single-source, service-based method for detection and identification of select Shiga toxin–producing Escherichia coli (STEC), including E. coli O157:H7 and STEC of somatic groups O26, O45, O103, O111, O121, and O145.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::249607eb3b17c21590bdb0080faf75bc
https://doi.org/10.5740/jaoacint.19-0300
https://doi.org/10.5740/jaoacint.19-0300
Autor:
Michael H. Brodsky, Linda Everis, Yvonne Salfinger, Yi Chen, Gail Betts, Aya Ogura, Hajime Teramura
Publikováno v:
Journal of AOAC International. 102:1502-1515
Background: Standard coliform count methods require preparation of agar, the use of pour-plate technique, the overlay of agar, and in some cases, the transfer of suspect colonies to broth medium for confirmation. The MC-Media Pad EC for enumeration o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::48b01c1ca7c8b9d0abad6dcc34b1a67c
https://doi.org/10.5740/jaoacint.19-0022
https://doi.org/10.5740/jaoacint.19-0022
Autor:
Michael H. Brodsky, Patrick Bird, Benjamin Bastin, David Higgins, Nevin Perera, M James Agin, Yvonne Salfinger, Yi Chen, David Goins, Simon Illingworth, Holly Urquhart, Siobhan Kelly
Publikováno v:
Journal of AOAC INTERNATIONAL. 102:1145-1161
Background: Solus One Salmonella is designed to accurately detect Salmonella species (Salmonella enterica subspecies enterica, salamae, arizonae, diarizonae, houtenae, indica, and Salmonella bongori) from select food matrixes and stainless-steel and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7b70d20f328e0f230637b919797ded5d
https://doi.org/10.5740/jaoacint.18-0345
https://doi.org/10.5740/jaoacint.18-0345
Autor:
Laura Cerqueira, James Agin, Patrick Bird, Michael H. Brodsky, Nuno F. Azevedo, Rui Rocha, Joseph Odumoru, J Mário Sousa, Carina Almeida, M Joseph Benzinger, David Goins, Benjamin Bastin, Yi Chen
Publikováno v:
Journal of AOAC International. 102:855-864
Background: Probe4Cronobacter test kit is based on the use of a fluorescence-labeled peptide nucleic acid probe (PNA) allied to fluorescence microscopy. A sample is taken after a 24 h enrichment of rehydrated 30 g portions of powdered infant formula
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::15fcb5ae4a4a69e58548465385a254c8
https://doi.org/10.5740/jaoacint.18-0328
https://doi.org/10.5740/jaoacint.18-0328