Zobrazeno 1 - 10
of 424
pro vyhledávání: '"H. -J. Apell"'
Publikováno v:
Journal of Membrane Biology. 156:63-71
The function of the Na,K-ATPase is known to be considerably impaired in the presence of free radicals such as OH.. While previous experiments were largely based on the loss of enzymatic activity of the protein, this is the first communication dealing
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e7b6ee2dc3b661ebd4ff8283b6c9762
https://doi.org/10.1007/s002329900188
https://doi.org/10.1007/s002329900188
The changes in capacitance and conductance of lipid bilayer membranes have been studied with adsorbed membrane fragments containing Na+,K+-ATPase. These changes have been initiated by fast release of protons from a bound form (“caged H+”) induced
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::339cc0d7d64042abe5f6217121da6048
Publikováno v:
Biophysical Journal. 61(1):83-95
In this paper the electrostatic interactions between membrane-embedded ion-pumps and their consequences for the kinetics of pump-mediated transport processes have been examined. We show that the time course of an intrinsically monomolecular transport
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2566d263abb203dea0fb2880ed6de6ec
Autor:
H.-J. Apell, Michael Habeck
Publikováno v:
Biophysical Journal. 96(3)
Electrochromic styryl dyes are in use now for almost two decades to detect ion movements in various P-type ATPases. The extremely hydrophobic dye molecules have a high partition coefficient in favor of the hydrophobic core of lipid phase of membrane
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df6662dee874e2d879cbdd41913cc741
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Biomembranes. 1023:81-90
Na,K-ATPase from rabbit kidney outer medulla was reconstituted in large unilamellar lipid vesicles by detergent dialysis. Vesicles prepared in the presence or absence of potassium allowed to study two different transport modes: the (physiological) Na
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63e3ce367e2e53b0640da7aeaf466d82
https://doi.org/10.1016/0005-2736(90)90012-d
https://doi.org/10.1016/0005-2736(90)90012-d
Autor:
H-J, Apell
Publikováno v:
Reviews of physiology, biochemistry and pharmacology. 150
P-type ATPases are a large family of membrane proteins that perform active ion transport across biological membranes. In these proteins the energy-providing ATP hydrolysis is coupled to ion-transport that builds up or maintains the electrochemical po
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::5f8532a87fc7c38f9100f8c0d165e16f
https://pubmed.ncbi.nlm.nih.gov/12811587
https://pubmed.ncbi.nlm.nih.gov/12811587
Publikováno v:
The Journal of biological chemistry. 275(3)
Based on the following observations we propose that the cytoplasmic loop between trans-membrane segments M6 and M7 (L6/7) of the alpha subunit of Na(+),K(+)-ATPase acts as an entrance port for Na(+) and K(+) ions. 1) In defined conditions chymotrypsi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::6cfa3eae84bdaeaa0b20b2eaa3f45719
https://pubmed.ncbi.nlm.nih.gov/10636905
https://pubmed.ncbi.nlm.nih.gov/10636905
Publikováno v:
Acta physiologica Scandinavica. Supplementum. 643
The complex functions of the Na,K-ATPase can be described by reaction cycles based on the generally accepted "Post-Albers cycle". By appropriate experimental conditions various, partly overlapping partial reactions may be isolated which allow the inv
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::62fd7ad82646ce57aeea280bc8be8568
https://pubmed.ncbi.nlm.nih.gov/9789566
https://pubmed.ncbi.nlm.nih.gov/9789566
Publikováno v:
Biophysical chemistry. 72(3)
The intrinsic fluorescence emission kinetics of Na,K-ATPase, a large membrane protein containing 16 tryptophan residues, was studied by time-resolved techniques. The lifetime distributions recovered by the Maximum Entropy Method exhibit a strong depe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4723e51b60f6482f8a9ecc04655af2fa
https://pubmed.ncbi.nlm.nih.gov/9691270
https://pubmed.ncbi.nlm.nih.gov/9691270
Publikováno v:
Membranecell biology. 11(5)
The goal of work was to investigate the electrogenic transport of Na ions by Na+,K(+)-ATPase in membrane fragments absorbed on a planar bilayer lipid membrane. The photorelease of ATP from an inactive precursor, caged ATP, induced a transient current
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e85b76d299b9e2b6d5144a082ecf06c3
https://pubmed.ncbi.nlm.nih.gov/9672883
https://pubmed.ncbi.nlm.nih.gov/9672883