Zobrazeno 1 - 10
of 46
pro vyhledávání: '"H Spieker-Polet"'
Publikováno v:
The Journal of Immunology. 152:3935-3945
B cells from leukemic rabbits preferentially use a single VH gene, VH1, in their VDJ gene rearrangements. To determine whether Ig-secreting B cells from normal rabbits also preferentially use VH1, we generated rabbit X mouse heterohybridomas that sta
Publikováno v:
The Journal of Immunology. 150:5457-5465
Molecular cloning techniques have recently demonstrated that rabbit has 13 different IgA C alpha H chain genes. This is in contrast to human and mouse that have only two and one C alpha heavy chain genes, respectively. In previous studies, nucleotide
Publikováno v:
Journal of immunology (Baltimore, Md. : 1950). 162(9)
The rabbit genome has 13 different Calpha genes that are expressed at different levels in mucosal tissues. To analyze the factors involved in the differential expression of these Calpha genes, we cloned and sequenced the promoters of the Ialpha regio
Publikováno v:
Leukemia. 8(12)
We developed transgenic rabbits with a DNA construct containing the proto-oncogene c-myc conjugated to the Ig kappa-chain enhancer gene, E kappa. One of four transgenic rabbits was mated to a normal rabbit and we used the offspring to develop a colon
Autor:
H Spieker-Polet, H Polet
Publikováno v:
Journal of Biological Chemistry. 251:987-992
Albumin from human, bovine, or rabbit serum supported the growth of concanavalin A-stimulated human thymus-derived lymphocytes equally well. This activity was completely abolished by pepsin digestion. It was shown for bovine serum albumin that the al
Autor:
H Spieker-Polet, H Polet
Publikováno v:
The Journal of Experimental Medicine
The effect of human plasma, the plasma protein fractions of Cohn, and crystallized serum albumin on the in vitro growth of human lymphocytes activated by concanavalin A (Con A) or bacterial lipopolysaccharide was compared. It was found that fraction
Autor:
H Spieker-Polet, H Polet
Publikováno v:
The Journal of Immunology. 126:949-954
Two FFA, 1 saturated and 1 unsaturated, and a FFA carrier protein are essential for in vitro growth of Con A-stimulated lymphocytes. Optimal growth was obtained with a combination of palmitic and oleic acid. As carrier proteins, serum albumin and bet
Publikováno v:
The Journal of Immunology. 134:2827-2834
It has been proposed that anti-Ig antibody activates B cells in a way analogous to the antigens, i.e., it delivers its signal by cross-linking and clustering the Ig receptors on the surface of one cell. However, the cross-linking of different B cells
Autor:
H, Polet, H, Spieker-Polet
Publikováno v:
The Journal of Immunology. 117:1275-1281
The concentration of concanavalin A (Con A) required for optimal growth of lymphocytes is 10 to 20 times greater in the presence of plasma than in the presence of serum albumin (BSA). This shift in Con A requirement is mainly caused by Cohn fractions
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