Zobrazeno 1 - 5
of 5
pro vyhledávání: '"H S, Rye"'
Publikováno v:
Cell. 97(3)
The double-ring chaperonin GroEL mediates protein folding in the central cavity of a ring bound by ATP and GroES, but it is unclear how GroEL cycles from one folding-active complex to the next. We observe that hydrolysis of ATP within the cis ring mu
Publikováno v:
Methods in enzymology. 290
Publikováno v:
The Journal of biological chemistry. 268(33)
The gel mobility-shift assay is an important tool for the study of protein-nucleic acid interactions. High detection sensitivity is typically attained by radioisotopic labeling of the target nucleic acid fragments. A novel fluorescence methodology of
Publikováno v:
Methods in enzymology. 217
The stable complexes between highly fluorescent, polyfunctional intercalators and dsDNA can be used to detect dsDNA in agarose gels at picogram levels and for multicolor detection of multiplexed dsDNA fragments. Development of additional DNA-binding
Publikováno v:
BioTechniques. 10(5)
A high-sensitivity, laser-excited confocal fluorescence gel scanner has been developed and applied to the detection of fluorescently labeled DNA. An argon ion laser (1-10 mW at 488 nm) is focused in the gel with a high-numerical aperture microscope o