Zobrazeno 1 - 10
of 19
pro vyhledávání: '"H S, Barra"'
Publikováno v:
Journal of Neuroscience Research. 28:171-181
We have used the monoclonal antibody YL 1/2 (Tyr) specific for tyrosinated tubulin, and a polyclonal antibody (Glu) specific for detyrosinated tubulin to visualize the distribution of microtubules and microtubule assembly sites during axonal outgrowt
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9eb67fbbf8a548dc058e125ad26a9b0b
https://doi.org/10.1002/jnr.490280204
https://doi.org/10.1002/jnr.490280204
Autor:
Carlos O. Arregui, H. S. Barra
Publikováno v:
Journal of Neuroscience Research. 27:256-263
We have used the monoclonal YL 1/2 (Tyr antibody) and polyclonal (Glu antibody) antibodies, specific for tyrosinated and detyrosinated tubulin, respectively, to determine the levels and cellular distribution of these tubulin species in chick retina d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29c9dee14c60e2b83cd5727481e98806
https://doi.org/10.1002/jnr.490270303
https://doi.org/10.1002/jnr.490270303
Publikováno v:
Scopus-Elsevier
The beta-amyloid peptide (beta AP1-40) inhibited the in vitro post-translational incorporation of [14C]arginine at the N-terminus of brain soluble proteins and was labelled by the incorporation of [14C]arginine. Addition of arginine at the N-terminal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04ba061328914be22937a79891767d69
https://doi.org/10.1097/00001756-199512290-00078
https://doi.org/10.1097/00001756-199512290-00078
Publikováno v:
Molecular and cellular biochemistry. 216(1-2)
Membranes from brain tissue contain tubulin that can be isolated as a hydrophobic compound by partitioning into Triton X-114. The hydrophobic behavior of this tubulin is due to the formation of a complex with the alpha-subunit of Na+,K+-ATPase. In th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::0c438edb06cf6c3ed5e0088ee85ed97e
https://pubmed.ncbi.nlm.nih.gov/11216868
https://pubmed.ncbi.nlm.nih.gov/11216868
Publikováno v:
Journal of neuroscience research. 56(1)
We have previously reported the posttranslational addition of [14C]-arginine in the N-terminus of several soluble rat brain proteins. One of these proteins was identified as the microtubule-associated protein, the stable tubule only polypeptide (STOP
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e7601db73862b79f826da3d4dd82111e
https://pubmed.ncbi.nlm.nih.gov/10213479
https://pubmed.ncbi.nlm.nih.gov/10213479
Publikováno v:
The Biochemical journal. 339
Tubulin carboxypeptidase is the enzyme that releases the C-terminal tyrosine from alpha-tubulin, converting tyrosine-terminated (Tyr) to detyrosinated (Glu) tubulin. The present study demonstrates that this enzyme is associated with microtubules in l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::6d2c89935c789923ef5ed66fcaaa26b6
https://pubmed.ncbi.nlm.nih.gov/10191280
https://pubmed.ncbi.nlm.nih.gov/10191280
Publikováno v:
Molecular and cellular biochemistry. 170(1-2)
Brain membranes contain tubulin that can be isolated as a hydrophobic compound by partitioning into Triton X-114. We have previously postulated: (a) that this kind of tubulin is a peripheral membrane protein that arises from microtubules that in vivo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::da7d407a420c505c55ddfd2852010871
https://pubmed.ncbi.nlm.nih.gov/9144322
https://pubmed.ncbi.nlm.nih.gov/9144322
Publikováno v:
Molecular and cellular biochemistry. 170(1-2)
Tubulin carboxypeptidase, the enzyme which releases the COOH terminal tyrosine from the alpha-chain of tubulin, remains associated with microtubules through several cycles of assembly/disassembly (Arce CA, Barra HS: FEBS Lett 157: 75-78, 1983). Here,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::63e1abfbf8866acc5497d3f2a1682a91
https://pubmed.ncbi.nlm.nih.gov/9144313
https://pubmed.ncbi.nlm.nih.gov/9144313
Publikováno v:
Molecular and cellular biochemistry. 170(1-2)
A preparation of tubulin carboxypeptidase partially purified from bovine brain was found to contain a protein of molecular mass 30 kDa (P30) as determined by SDS-PAGE, that is recognized by a polyclonal anti-bovine pancreatic carboxypeptidase A. Howe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::57e6dbea1bc3b5f44eca10637332f882
https://pubmed.ncbi.nlm.nih.gov/9144328
https://pubmed.ncbi.nlm.nih.gov/9144328
Publikováno v:
Neurochemical research. 22(4)
We demonstrate here that brain purified tubulin can be dissociated into alpha and beta subunits at pH10 and that the subunits can be separated by using the Triton X-114 phase separation system. After phase partition at pH10, alpha tubulin but not bet
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::f103c5b4d6b416c33a77f2d4e25113e5
https://pubmed.ncbi.nlm.nih.gov/9130248
https://pubmed.ncbi.nlm.nih.gov/9130248