Zobrazeno 1 - 10
of 70
pro vyhledávání: '"H L, Pardue"'
Autor:
H L, Pardue
Publikováno v:
Clinical chemistry. 43(10)
The formal definition of sensitivity associates the term with the change in the response of a system for a small change of the stimulus causing the response, i.e., the ratio of the response of a system to the stimulus causing it. One interpretation o
Publikováno v:
Annales de biologie clinique. 52(5)
Publikováno v:
Annales de biologie clinique. 52(4)
This paper introduces a systematic approach to organizing the discipline of clinical chemistry. The approach is called a top-down, systems approach because it starts at the top with the most general concepts and works down through less general concep
Autor:
K B, Lim, H L, Pardue
Publikováno v:
Clinical chemistry. 39(9)
We describe the adaptation and evaluation of an error-compensating method for kinetic determinations of deoxyribonucleic acids (DNAs). The DNA is first reacted with ethidium bromide to produce a fluorescent intercalation complex. Subsequent treatment
Autor:
C E, Uhegbu, H L, Pardue
Publikováno v:
Analytical chemistry. 64(20)
This paper describes the use of a predictive, curve-fitting method to reduce the effects of experimental variables on results obtained with membrane-based devices. Multipoint data from the transient regions of responses are used with suitable models
Autor:
C P, Fitzpatrick, H L, Pardue
Publikováno v:
Clinical chemistry. 38(2)
We describe a kinetic method for the determination of alkaline phosphatase (ALP) isoenzymes, based on fluorescence detection of 4-methylumbelliferone. Several different buffer-inhibitor combinations and substrate concentrations were evaluated. Best r
Autor:
C P, Fitzpatrick, H L, Pardue
Publikováno v:
Clinical chemistry. 38(2)
We describe an approach for the simultaneous determination of isoenzymes of alkaline phosphatase (EC 3.1.3.1) based on the kinetic behavior of inhibition reactions. Data for absorbance vs time, collected while enzymes are being inhibited, are fitted
Autor:
B L, Bacon, H L, Pardue
Publikováno v:
Clinical chemistry. 37(8)
Here we describe an error-compensating kinetic-based method for the enzymatic quantification of creatinine in serum. The method, which has a large linear range and very low dependency on experimental variables that influence enzyme activity, is based
Publikováno v:
Annales de biologie clinique. 49(10)
Increasing numbers of robots are going to be employed industrial chemical laboratories. Most of these will be used to reduce the monotonous tasks of sample preparation, to minimize human exposure to dangerous environments or to carry out huge numbers
Autor:
H L Pardue, S D Hamilton
Publikováno v:
Clinical Chemistry. 30:226-229
We report conditions and characteristics of a new kinetic method for measuring lactate. Using a multiple linear regression method, we fit data for absorbance and for rate of change of absorbance to a modified rate form of the Michaelis-Menten equatio