Zobrazeno 1 - 10
of 490
pro vyhledávání: '"H G, Khorana"'
The photosensitive m-diazirinophenyl analog of retinal (Fig. 1, II) bound to bacterio-opsin at Lys-216 and regenerated a chromophore with lambda max at 470 nm. Photolysis of the complex at 365 nm resulted in covalent cross-linking of the retinal anal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca5e302f55822384caff5cb6fe3290e0
https://ora.ox.ac.uk/objects/uuid:f5be2100-9ce0-4996-93bb-44fbef723efa
https://ora.ox.ac.uk/objects/uuid:f5be2100-9ce0-4996-93bb-44fbef723efa
The complete denaturation and subsequent renaturation and reconstitution of a polytopic integral membrane protein are demonstrated. Delipidated bacteriorhodopsin (Huang, K.-S., Bayley, H., and Khorana, H. G. (1980) Proc. Natl. Acad. Sci. U. S. A. 77,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77c3c8ff0693528e91981c4a014585ff
https://ora.ox.ac.uk/objects/uuid:4fb0feb6-58e3-4bbd-8c4f-5b8d2b0b929b
https://ora.ox.ac.uk/objects/uuid:4fb0feb6-58e3-4bbd-8c4f-5b8d2b0b929b
Publikováno v:
Annals of the New York Academy of Sciences. 81:757-775
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eea95e8cec1ceb8af5f64f4bed2f99a2
https://doi.org/10.1111/j.1749-6632.1959.tb49357.x
https://doi.org/10.1111/j.1749-6632.1959.tb49357.x
Publikováno v:
Biochemistry. 44:2284-2292
Structure-function studies of rhodopsin indicate that both intradiscal and transmembrane (TM) domains are required for retinal binding and subsequent light-induced structural changes in the cytoplasmic domain. Further, a hypothesis involving a common
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72d4ac5f3eb20a659eb92062c95a7d41
https://doi.org/10.1021/bi048328i
https://doi.org/10.1021/bi048328i
Publikováno v:
Journal of Biological Chemistry. 278:39020-39028
L125R, a severe retinitis pigmentosa rhodopsin missense mutation, results in rhodopsin protein misfolding, retinal degeneration, and ultimately blindness. The initiating structural events leading to this protein misfolding are unknown. Through the us
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::54ea2bc3db4cc50834cdbc8a0069f7d8
https://doi.org/10.1074/jbc.m303625200
https://doi.org/10.1074/jbc.m303625200
Autor:
Michele C. Loewen, J. Chung, H G Khorana, Harald Schwalbe, E V Getmanova, Judith Klein-Seetharaman, Philip J. Reeves, P. E. Wright
Publikováno v:
Proceedings of the National Academy of Sciences. 99:3452-3457
[α- 15 N]Lysine-labeled rhodopsin, prepared by expression of a synthetic gene in HEK293 cells, was investigated both by conventional and transverse relaxation optimized spectroscopy-type heteronuclear single quantum correlation spectroscopy. Whereas
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9af9dcc96992c8507a727eeccf637792
https://doi.org/10.1073/pnas.052713999
https://doi.org/10.1073/pnas.052713999
Autor:
H. G. Khorana
Biochemical studies of protein synthesis in vitro got under way in the early 1950s. The fundamental concept arose that the specificity in protein synthesis was primarily governed by the loading of every amino acid onto a "cognate" soluble RNA by an e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cfef0411807969359af8141cdd9d8808
https://doi.org/10.1128/9781555818333.ch2
https://doi.org/10.1128/9781555818333.ch2
Publikováno v:
Journal of Molecular Biology. 308:423-435
The loops connecting the seven transmembrane helices of bacteriorhodopsin have each been replaced in turn by structureless linkers of Gly-Gly-Ser repeat sequences, and the effect on the protein folding kinetics has been determined. An SDS-denatured s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::38a0aacaf4de7bcb74a60bcd11f3b29a
https://doi.org/10.1006/jmbi.2001.4604
https://doi.org/10.1006/jmbi.2001.4604
Publikováno v:
Journal of Molecular Biology. 308:409-422
Bacteriorhodopsin functions as a light-driven proton pump in Halobacterium salinarium. The functional protein consists of an apoprotein, bacterioopsin, with seven transmembrane alpha helices together with a covalently bound all-trans retinal chromoph
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::665d416a22b77f9b4f37d8087603220a
https://doi.org/10.1006/jmbi.2001.4603
https://doi.org/10.1006/jmbi.2001.4603
Publikováno v:
Proceedings of the National Academy of Sciences. 97:3004-3009
A suitable system for expression of the rhodopsin kinase (RK) gene and its mutants is needed for structure–function studies of RK. Previously, investigation of the baculovirus system showed satisfactory production of RK, but posttranslational isopr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c95e4845361bf70ee27dde0ab6941be6
https://doi.org/10.1073/pnas.97.7.3004
https://doi.org/10.1073/pnas.97.7.3004