Zobrazeno 1 - 10
of 111
pro vyhledávání: '"H D, Fahimi"'
Publikováno v:
Cytogenetic and Genome Research. 103:360-365
Peroxisomes are cell organelles that perform multiple functions in the metabolism of lipids and of reactive oxygen species. They are present in most eukaryotic cells. However, they are believed to be absent in spermatozoa and they have never been des
Publikováno v:
Scopus-Elsevier
SUMMARY We present a protocol for detection of peroxisomal proteins and their corresponding mRNAs on consecutive serial sections of fetal and newborn mouse tissues by immunohistochemistry (IHC) and nonradioactive in situ hybridization (ISH). The use
Publikováno v:
Cell Biochemistry and Biophysics. 32:37-49
Peroxisomes in the human hepatoblastoma cell line, HepG2, exhibit distinct alterations of shape, size, and distribution, dependent on culture conditions (cell density, duration in culture, and presence of specific growth factors). Although many cells
Publikováno v:
Annals of the New York Academy of Sciences. 887:12-17
The electron microscopical studies presented here show that characteristic morphological alterations in mitochondria are a very early hallmark of the hepatocellular apoptotic program. Before chromatin condensation occurs, the outer mitochondrial memb
Autor:
Steinmetz I, Czerny F, H D Fahimi, Klaus Kusterer, Ernst Hanisch, Konstantin Beier, Alfred Völkl, Sabine Angermüller, Thomas Weber
Publikováno v:
Transplantation. 66:186-194
BACKGROUND During allograft rejection, cytokines and lipid mediators contribute to cell injury and organ failure. Peroxisomes play a crucial role in lipid metabolism, including the degradation of lipid mediators by peroxisomal beta-oxidation. Therefo
Publikováno v:
BioTechniques. 22:500-505
We have developed a simple and sensitive method for the rapid quantitation of mRNA from cell cultures and small tissue samples. The method combines the high sensitivity and specificity of the ribonuclease protection assay with simple handling and rap
Molecular cloning and further characterization of rat peroxisomal trihydroxycoprostanoyl-CoA oxidase
Autor:
Johannes C. T. Vanhooren, Eveline Baumgart, Marc Fransen, F. Van Leuven, H D Fahimi, Guy P. Mannaerts, P. P. Van Veldhoven
Publikováno v:
Scopus-Elsevier
The composite trihydroxycoprostanoyl-CoA oxidase cDNA sequence, derived from overlapping clones isolated via screening of two different rat liver expression libraries, consisted of 2509 bases and contained an open reading frame of 2046 bases, encodin
Publikováno v:
Histochemistry and Cell Biology. 106:105-114
The immunogold labeling technique has been extremely useful in investigation of the structure and function of peroxisomes. In this report a few examples of the application of this technique with significant implications in the field are briefly revie
Publikováno v:
Journal of Biological Chemistry. 271:3846-3855
The subcellular localization of L-lactate dehydrogenase (LDH) in rat hepatocytes has been studied by analytical subcellular fractionation combined with the immunodetection of LDH in isolated subcellular fractions and liver sections by immunoblotting
Publikováno v:
Histochemistry and Cell Biology. 103:187-195
We have compared the effects of fixation with three commonly used fixatives upon preservation of the antigenicity of six peroxisomal proteins in rat liver using both immunohistochemical staining and Western blotting of fixed tissue extracts. The immu