Zobrazeno 1 - 10
of 73
pro vyhledávání: '"H A, Huysmans"'
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Excitatory amino acid transporters (EAATs) are crucial for the removal of excitatory amino acids from the synaptic cleft. Here authors combined high-speed atomic force microscopy line-scanning with automated state assignment for the determination of
Externí odkaz:
https://doaj.org/article/c95aa1629c334ed0810a6e777c977f72
Autor:
Xiaojun Yuan, Matthew D. Johnson, Jing Zhang, Alvin W. Lo, Mark A. Schembri, Lakshmi C. Wijeyewickrema, Robert N. Pike, Gerard H. M. Huysmans, Ian R. Henderson, Denisse L. Leyton
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-12 (2018)
Autotransporter passenger domains are presented on or released from the bacterial surface upon translocation through an outer membrane β-barrel anchor. Here the authors study the two E. coli autotransporters Pet and EspP and propose that the β-barr
Externí odkaz:
https://doaj.org/article/f98a1b1242774a1e9a6565cc46622c09
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2168
The thermodynamic stabilities of membrane proteins are of fundamental interest to provide a biophysical description of their structure-function relationships because energy determines conformational populations. In addition, structure-energy relation
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::f2ce9e620ea78f958b1c2bf88d92ddd9
https://pubmed.ncbi.nlm.nih.gov/33582994
https://pubmed.ncbi.nlm.nih.gov/33582994
Autor:
Daniel S. Terry, Scott C. Blanchard, Changhao He, Xiaoyu Wang, Gabriel A. Fitzgerald, Olga Boudker, Didar Ciftci, Gerard H. M. Huysmans, Zhou Zhou
Publikováno v:
Bio Protoc
Secondary active transporters reside in cell membranes transporting polar solutes like amino acids against steep concentration gradients, using electrochemical gradients of ions as energy sources. Commonly, ensemble-based measurements of radiolabeled
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc073b022f06579d71a318e3a479b64b
https://doi.org/10.21769/bioprotoc.3970
https://doi.org/10.21769/bioprotoc.3970
Publikováno v:
The EMBO Journal
Membrane transporters mediate cellular uptake of nutrients, signaling molecules, and drugs. Their overall mechanisms are often well understood, but the structural features setting their rates are mostly unknown. Earlier single‐molecule fluorescence
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::828439ddbc9fe8003f5a99ca6ccfaef3
https://doi.org/10.15252/embj.2020105415
https://doi.org/10.15252/embj.2020105415
Autor:
Scott C. Blanchard, Olga Boudker, Changhao He, Gabriel A. Fitzgerald, Zhou Zhou, Didar Ciftci, Gerard H. M. Huysmans, Daniel S. Terry, Xiaoyu Wang
Publikováno v:
Science Advances
Single-molecule detection of transport turnovers shows a distribution of rates with fast transporters favored by mutations.
Kinetic properties of membrane transporters are typically poorly defined because high-resolution functional assays analog
Kinetic properties of membrane transporters are typically poorly defined because high-resolution functional assays analog
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::beda62e3dfeb0ed2b4646768a709fb41
http://europepmc.org/articles/PMC7259943
http://europepmc.org/articles/PMC7259943
Membrane transporters mediate cellular uptake of nutrients, signaling molecules and drugs. Their overall mechanisms are often well understood, but the structural features setting their rates are mostly unknown. Earlier single-molecule fluorescence im
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d2a3fbae0b52f21355f795fbd439bf5
https://doi.org/10.1101/2020.04.17.047373
https://doi.org/10.1101/2020.04.17.047373
Publikováno v:
Methods in Molecular Biology ISBN: 9781071607237
The thermodynamic stabilities of membrane proteins are of fundamental interest to provide a biophysical description of their structure-function relationships because energy determines conformational populations. In addition, structure-energy relation
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fca7107cd87db74e24d13dc3cc2840e5
https://doi.org/10.1007/978-1-0716-0724-4_10
https://doi.org/10.1007/978-1-0716-0724-4_10
Autor:
Yun Huang, Clay Bracken, Harel Weinstein, David Eliezer, Gerard H. M. Huysmans, Olga Boudker, Asghar M. Razavi, Xiaoyu Wang, Guohua Lv
Publikováno v:
Nature Chemical Biology
In proteins where conformational changes are functionally important, the number of accessible states and their dynamics are often difficult to establish. Here we describe a novel 19F-NMR spectroscopy approach to probe dynamics of large membrane prote
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7344f9bee1843a8b3d52f405794fde0f
Publikováno v:
Nature Communications
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Excitatory amino acid transporters (EAATs) are important in many physiological processes and crucial for the removal of excitatory amino acids from the synaptic cleft. Here, we develop and apply high-speed atomic force microscopy line-scanning (HS-AF
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f8aab87bb7c7b66fa020dca57c7075e