Výsledky vyhledávání - "Hélène Gaussier"

Functional characterization and FTIR-based 3D modeling of full length and truncated forms of Scorpio maurus venom phospholipase A 2

Autor: Hélène Gaussier, Hanen Louati, Frédéric Carrière, Raida Jallouli, Goetz Parsiegla, Pascal Mansuelle, Najeh Krayem, Youssef Gargouri

Publikováno v: Biochimica et Biophysica Acta (BBA)-General Subjects
Biochimica et Biophysica Acta (BBA)-General Subjects, 2018, 1862 (5), pp.1247-1261. ⟨10.1016/j.bbagen.2018.02.004⟩
Biochimica et Biophysica Acta (BBA)-General Subjects, Elsevier, 2018, 1862 (5), pp.1247-1261. ⟨10.1016/j.bbagen.2018.02.004⟩

International audience; Background: Heterodimeric phospholipase A2 from venom glands of Tunisian scorpion Scorpio maurus (Sm-PLGV) had been purified. It contains long and short chains linked by a disulfide bridge. Sm-PLGV exhibits hemolytic activity

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93939892f610b092698e321c1e4c49b2
https://doi.org/10.1016/j.bbagen.2018.02.004

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IR spectroscopy analysis of pancreatic lipase-related protein 2 interaction with phospholipids: 1. Discriminative recognition of mixed micelles versus liposomes

Autor: Sylvie Robert, Jean-Claude Bakala N’Goma, Frédéric Carrière, Eduardo Mateos-Diaz, Deborah Byrne, Hélène Gaussier

Publikováno v: Chemistry and Physics of Lipids
Chemistry and Physics of Lipids, Elsevier, 2018, 211, pp.52-65. ⟨10.1016/j.chemphyslip.2017.02.005⟩
Chemistry and Physics of Lipids, 2018, 211, pp.52-65. ⟨10.1016/j.chemphyslip.2017.02.005⟩
Chemistry and Physics of Lipids, Elsevier, 2017, IN PRESS
Chemistry and Physics of Lipids, 2017, IN PRESS

International audience; Guinea pig pancreatic lipase-related protein 2 (GPLRP2) is an interesting model enzyme that can hydrolyze a large set of acylglycerols in vitro but displays however some selectivity depending on the supramolecular structure of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5189d509ee83c05fea4ac188b209acba
https://doi.org/10.1016/j.chemphyslip.2017.02.005

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IR spectroscopy analysis of pancreatic lipase-related protein 2 interaction with phospholipids: 2. Discriminative recognition of various micellar systems and characterization of PLRP2-DPPC-bile salt complexes

Autor: Hélène Gaussier, Frédéric Carrière, Deborah Byrne, Eduardo Mateos-Diaz, Moulay Sahaka, Priscila Sutto-Ortiz

Publikováno v: Chemistry and Physics of Lipids
Chemistry and Physics of Lipids, Elsevier, 2018, 211, pp.66-76. ⟨10.1016/j.chemphyslip.2017.11.012⟩
Chemistry and Physics of Lipids, 2018, 211, pp.66-76. ⟨10.1016/j.chemphyslip.2017.11.012⟩

International audience; The interaction of pancreatic lipase-related protein 2 (PLRP2) with various micelles containing phospholipids was investigated using pHstat enzyme activity measurements, differential light scattering, size exclusion chromatogr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a7466561e88e16bdbc161008112c53fa
https://doi.org/10.1016/j.chemphyslip.2017.11.012

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Functional characterization and FTIR-based 3D modeling of full length and truncated forms of Scorpio maurus venom phospholipase A

Autor: Najeh, Krayem, Goetz, Parsiegla, Hélène, Gaussier, Hanen, Louati, Raida, Jallouli, Pascal, Mansuelle, Frédéric, Carrière, Youssef, Gargouri

Publikováno v: Biochimica et biophysica acta. General subjects. 1862(5)

Heterodimeric phospholipase AMolecular cloning of Sm-PLGV and heterologous expression in Escherichia coli of three recombinant forms was used to determine the role of the short chain on enzymatic activity. Infrared spectroscopy assisted 3D model buil

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::aaf925e2ac91160d8aa7333d515b13eb
https://pubmed.ncbi.nlm.nih.gov/29477749

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The DNA Maturation Domain of gpA, the DNA Packaging Motor Protein of Bacteriophage Lambda, Contains an ATPase Site Associated with Endonuclease Activity

Autor: Hélène Gaussier, Marcos E. Ortega, Carlos Enrique Catalano

Publikováno v: Journal of Molecular Biology. 373:851-865

Terminase enzymes are common to double-stranded DNA (dsDNA) viruses and are responsible for packaging viral DNA into the confines of an empty capsid shell. In bacteriophage lambda the catalytic terminase subunit is gpA, which is responsible for matur

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d131f4e6ac6a431ec64d3020b6f497f6
https://doi.org/10.1016/j.jmb.2007.07.067

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Assembly of Bacteriophage Lambda Terminase into a Viral DNA Maturation and Packaging Machine

Autor: Elke Bogner, Nasib K. Maluf, Carlos Enrique Catalano, Hélène Gaussier, Michael Feiss

Publikováno v: Biochemistry. 45:15259-15268

Terminase enzymes are common to complex double-stranded DNA viruses and function to package viral DNA into the capsid. We recently demonstrated that the bacteriophage lambda terminase gpA and gpNu1 proteins assemble into a stable heterotrimer with a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5fd3cc27c480f0360ca9e018b142a5d4
https://doi.org/10.1021/bi0615036

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Building a Virus from Scratch: Assembly of an Infectious Virus Using Purified Components in a Rigorously Defined Biochemical Assay System

Autor: Hélène Gaussier, Qin Yang, Carlos Enrique Catalano

Publikováno v: Journal of Molecular Biology. 357:1154-1166

The assembly of double-stranded DNA (dsDNA) viruses such as poxvirus, the herpesviruses and many bacteriophages is a complex process that requires the coordinated activities of numerous proteins of both viral and host origin. Here, we report the asse

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f459fee82aefdaa1b2eee4e374b73ee4
https://doi.org/10.1016/j.jmb.2006.01.013

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Binding of Pediocin PA-1 with Anionic Lipid Induces Model Membrane Destabilization

Autor: Hélène Gaussier, Muriel Subirade, Thierry Lefèvre

Publikováno v: Applied and Environmental Microbiology. 69:6777-6784

To obtain molecular insights into the action mode of antimicrobial activity of pediocin PA-1, the interactions between this bacteriocin and dimyristoylphosphatidylcholine (DMPC) or dimyristoylphosphatidylglycerol (DMPG) model membranes have been inve

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e68c2f45bc1a4e5673cec73a6899f902
https://doi.org/10.1128/aem.69.11.6777-6784.2003

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Conformational changes of pediocin in an aqueous medium monitored by Fourier transform infrared spectroscopy: a biological implication

Autor: Marc C. Lavoie, Hélène Gaussier, Muriel Subirade

Publikováno v: International Journal of Biological Macromolecules. 32:1-9

Fourier transform infrared (FTIR) spectroscopy was used to investigate the secondary structure of pediocin PA-1 in different aqueous media in relation to its antimicrobial activity. The experiments were performed at pD (pH meter corrected for deuteri

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b0554de4c67195e953be45285d3c0fcb
https://doi.org/10.1016/s0141-8130(03)00018-7

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Replacement of Trifluoroacetic Acid with HCl in the Hydrophobic Purification Steps of Pediocin PA-1: a Structural Effect

Autor: Marc C. Lavoie, Hélène Gaussier, Muriel Subirade, Hélène Morency

Publikováno v: Applied and Environmental Microbiology. 68:4803-4808

Trifluoroacetic acid (TFA) is a purification contaminant associated with pediocin PA-1 that interferes with Fourier transform infrared spectroscopy structural analysis. As revealed by circular dichroism, its presence affects the structural folding of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::095ca22536d79b9046ffb34ff4dfd881
https://doi.org/10.1128/aem.68.10.4803-4808.2002

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