High yield tissue dispersing method for cell culture

Autor: Héctor Montes de Oca

Publikováno v: Journal of Tissue Culture Methods. 6:97-100

A reliable method for preparing primary cultures from different organs, using a mixture of trypsin and Versene as dispersing agent(s) is described. This method provides high cell yields as well as monolayers free of cellular debris and suitable for d

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::69f00ac04a3050c2d310b77196fbb1b3
https://doi.org/10.1007/bf02082857

List of Contributors

Autor: NABIL ABAZA, MARLENE ABSHER, BYRON TH. AFTONOMOS, CORINE R. ANDERSEN, FRANCES E. ARRIGHI, S. AVRAMEAS, RALPH BAKER, MICHAEL F. BARILE, G. BARSKI, ANNIE R. BEASLEY, J. BELEHRADEK, CHARLES W. BOONE, J. BORNSTEIN, MURRAY B. BORNSTEIN, CHAIM BRAUTBAR, W.E. BRIDSON, B.R. BRINKLEY, LAROY N. CASTOR, JEFFREY P. CHANG, JESSE CHARNEY, WILLIAM R. CHERRY, H FRED CLARK, D.O. CLIVER, E. CONWAY, JOHN E.K. COOPER, LEWIS L. CORIELL, RODY P. Cox, V.J. CRISTOFALO, C.H. CUNNINGHAM, H. DALEN, W.F. DALY, JOSEPH DANCIS, B. SHANNON DANES, P.S. DANNIES, A.D. DEITCH, R.T. DELL'ORCO, D.K. DOUGALL, GERALD M. EDELMAN, MAGDALENA EISINGER, WILLIAM J. EISLER, VIRGINIA J. EVANS, L. FALK, ROSANN A. FARBER, S. FEDOROFF, H. FIRKET, JOHN F. FOLEY, JEROME J. FREED, JAMES G. GALLAGHER, O.L. GAMBORG, STANLEY M. GARTLER, K.M. GAUTVIK, NAZARETH GENGOZIAN, RICHARD E. GILES, G.C. GODMAN, B. GOLDBERG, ARTHUR E. GREENE, P.M. GULLINO, RALPH B.L. GWATKIN, A.J. HACKETT, RICHARD G. HAM, M. HARRIS, LEONARD HAYFLICK, CHARLES HEIDELBERGER, RENÉ HELLER, A.C. HILDEBRANDT, S. ROBERT HILFER, H.T. HOLDEN, WILLIAM HOUSE, T.C. Hsu, ROBERT N. HULL, JOEL HUTZLER, JOAN KABAKJIAN, R.H. KAHN, M. EDWARD KAIGHN, S.S. KALTER, K.N. KAO, FREDERICK H. KASTEN, L.N. KEEN, HAROLD KINNAMAN, R.A. KNAZEK, P.O. KOHLER, G.M. KOLODNY, DAVID F. KRAHN, B. KRAMARSKY, ROBERT S. KROOTH, P.F. KRUSE, W.G.W. KURZ, B.L. LARSON, E.Y. LASFARGUES, JOHN C. LEE, JOSEPH LEIGHTON, W. LICHTER, C.C. LIN, JACK LITWIN, LAURA S. MCKENZIE, E. CHURCHILL MCKINNEY, IAN MACPHERSON, A. MACIEIRA-COELHO, MARVIN L. MACY, SUNDER MANSUKHANI, E.P. MARKS, G.M. MARTIN, M.C. MATHES, YUJI MATSUOKA, M.D. MAXWELL, W.J. MELLMAN, P.J. MELNICK, J.G. MERTENS, LISELOTTE MEZGER-FREED, R.A. MILLER, A.F. MIRANDA, J.M. MOEHRING, MARGARET A.J. MOFFAT, HÉCTOR MONTES DE OCA, PAUL S. MOORHEAD, JOSEPH F. MORGAN, HELEN J. MORTON, T. MURASHIGE, CARL R. PARTANEN, M.K. PATTERSON, ROLAND A. PATTILLO, CARL PERAINO, HUGH J. PHILLIPS, JAN PONTÉN, RAYMOND P. PORTER, F.J.A. PROP, R.W. PUMPER, LEONARD J. QUADRACCI, Y. RABINOWITZ, F. RAPP, R. REYNOLDS, CATHRINE A. REZNIKOFF, ALAN RICHTER, JAMES A. ROBB, E. ROBBINS, GEORGE G. ROSE, LEONARD J. ROSENTHAL, D.E. ROUNDS, H. RUBIN, FRANK H. RUDDLE, K.K. SANFORD, M.A. SAVAGEAU, JOSEPH B. SCHLEICHER, IMOGENE SCHNEIDER, BERNARD SCHWARTZ, P.L. SCHWARTZ, ELIZABETH K. SELL, SYDNEY SHALL, JOHN E. SHANNON, ISAAC L. SHECHMEISTER, CHARLES SHIPMAN, G. SHRAMEK, M. MICHAEL SIGEL, W.F. SIMPSON, ROBERT F. SMITH, T.A. STEEVES, N.G. STEPHENSON, W.R. STINEBRING, H.E. STREET, GARY E. STRIKER, C.S. STULBERG, J.A. SYKES, A.H. TASHJIAN, WILLIAM G. TAYLOR, T.A. TEDESCO, PHILIP S. THAYER, JOHN G. TORREY, IRENE A. UCHIDA, CARLO VALENTI, J. VAN'T HOF, A.L. VAN WEZEL, I.K. VASIL, JAMES L. VAUGHN, H.C. WANG, CHARITY WAYMOUTH, GEORGE WEAVER, R.E.H. WETTENHALL, W.L. WHITTLE, G.J. WIEPJES, L.L. WINTON, KEN WOLF, DAVID YAFFE

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::762ef0cafe8e42e69bf680d4c4ad33a1
https://doi.org/10.1016/b978-0-12-427150-0.50003-6