Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Gwynedd A. Benders"'
Autor:
Gwynedd A. Benders, Cynthia Andrews-Pfannkoch, Daniel G. Gibson, Bogumil J. Karas, Pratap Venepally, John I. Glass, Clyde A. Hutchison, Hamilton O. Smith, Ray-Yuan Chuang, Christopher L. Dupont, Adi Ramon, Mark Novotny, Li Ma, Michael G. Montague, Ariel S. Schwartz, J. Craig Venter, Daniel Brami, Christian Tagwerker
Publikováno v:
Nucleic Acids Research
Marine cyanobacteria of the genus Prochlorococcus represent numerically dominant photoautotrophs residing throughout the euphotic zones in the open oceans and are major contributors to the global carbon cycle. Prochlorococcus has remained a genetical
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24f288e1add93d59fcc996ba9a6f7215
https://doi.org/10.1093/nar/gks823
https://doi.org/10.1093/nar/gks823
Autor:
Nacyra Assad-Garcia, J. Craig Venter, Zhi-Qing Qi, Prashanth P. Parmar, Ray-Yuan Chuang, Clyde A. Hutchison, Mikkel A. Algire, Christopher H. Calvey, Hamilton O. Smith, Evgeniya A. Denisova, Vladimir N. Noskov, Monzia Moodie, Daniel G. Gibson, Chuck Merryman, Carole Lartigue, Li Ma, Radha Krishnakumar, Gwynedd A. Benders, Michael G. Montague, Cynthia Andrews-Pfannkoch, Lei Young, John I. Glass, Sanjay Vashee, Thomas H. Segall-Shapiro
Publikováno v:
Science
Science, American Association for the Advancement of Science, 2010, 329 (5987), pp.52-56. ⟨10.1126/science.1190719⟩
Science, American Association for the Advancement of Science, 2010, 329 (5987), pp.52-56. ⟨10.1126/science.1190719⟩
Let There Be Life The DNA sequence information from thousands of genomes is stored digitally as ones and zeros in computer memory. Now, Gibson et al. (p. 52 , published online 20 May; see the cover; see the Policy Forum by Cho and Relman ) have broug
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6ec6a39f2acbbef4e75e92dc0df048e
https://doi.org/10.1126/science.1190719
https://doi.org/10.1126/science.1190719
Autor:
Hamilton O. Smith, Monzia Moodie, Mikkel A. Algire, Daniel G. Gibson, Sanjay Vashee, Quang Phan, Nacyra Assad-Garcia, John I. Glass, Clyde A. Hutchison, Ray-Yuan Chuang, Gwynedd A. Benders, Carole Lartigue, J. Craig Venter, Chuck Merryman, Vladimir N. Noskov, Nina Alperovich, Evgeniya A. Denisova, William Carrera
Publikováno v:
Nucleic Acids Research
Nucleic Acids Research, Oxford University Press, 2010, 38 (8), pp.2558-2569. ⟨10.1093/nar/gkq119⟩
Nucleic Acids Research, Oxford University Press, 2010, 38 (8), pp.2558-2569. ⟨10.1093/nar/gkq119⟩
Most microbes have not been cultured, and many of those that are cultivatable are difficult, dangerous or expensive to propagate or are genetically intractable. Routine cloning of large genome fractions or whole genomes from these organisms would sig
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a8cc00e7dd1a602876b918497dafc14
http://europepmc.org/articles/PMC2860123
http://europepmc.org/articles/PMC2860123
Autor:
Vladimir N. Noskov, Cynthia Andrews-Pfannkoch, Hamilton O. Smith, Mikkel A. Algire, Timothy B. Stockwell, Holly Baden-Tillson, Chuck Merryman, Lei Young, Evgeniya A. Denisova, Daniel G. Gibson, J. Craig Venter, David W. Thomas, Jayshree Zaveri, Clyde A. Hutchison, Gwynedd A. Benders, Anushka Brownley, John I. Glass
Publikováno v:
Science. 319:1215-1220
We have synthesized a 582,970–base pair Mycoplasma genitalium genome. This synthetic genome, named M. genitalium JCVI-1.0, contains all the genes of wild-type M. genitalium G37 except MG408, which was disrupted by an antibiotic marker to block path
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e328b75b3b24245c07b90d0077597f58
https://doi.org/10.1126/science.1151721
https://doi.org/10.1126/science.1151721
Autor:
Gwynedd A, Benders
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 852
Many bacterial and archaeal genomes are of a similar size to molecules that have been cloned in the yeast Saccharomyces cerevisiae and thus might be clonable as single, circular episomes in this host. Yeast offers a variety of efficient tools for the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::1e0e45bbd73176590d6dc24bc1fe51d7
https://pubmed.ncbi.nlm.nih.gov/22328433
https://pubmed.ncbi.nlm.nih.gov/22328433
Autor:
Gwynedd A. Benders
Publikováno v:
Methods in Molecular Biology ISBN: 9781617795633
Many bacterial and archaeal genomes are of a similar size to molecules that have been cloned in the yeast Saccharomyces cerevisiae and thus might be clonable as single, circular episomes in this host. Yeast offers a variety of efficient tools for the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a8b1da695903d826c33cd551cd9d435d
https://doi.org/10.1007/978-1-61779-564-0_13
https://doi.org/10.1007/978-1-61779-564-0_13
Autor:
Michael G. Montague, John I. Glass, Radha Krishnakumar, Quang Phan, Gwynedd A. Benders, Nacyra Assad-Garcia
Publikováno v:
Applied and environmental microbiology. 76(15)
Most gene knockouts in mycoplasmas are achieved through labor-intensive transposon mutagenesis. Here, we describe a method for making targeted deletions in Mycoplasma pneumoniae by use of homologous recombination. In this method, M. pneumoniae is tra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a13d88e1313a3cf6a4355cd6dc222fd0
https://pubmed.ncbi.nlm.nih.gov/20543037
https://pubmed.ncbi.nlm.nih.gov/20543037
Autor:
Nina Alperovich, Vladimir N. Noskov, John I. Glass, Evgeniya A. Denisova, Chuck Merryman, Daniel G. Gibson, Sanjay Vashee, Carole Lartigue, Li Ma, Hamilton O. Smith, Mikkel A. Algire, Gwynedd A. Benders, David W. Thomas, Clyde A. Hutchison, Nacyra Assad-Garcia, J. Craig Venter, Ray-Yuan Chuang
Publikováno v:
Science
Science, American Association for the Advancement of Science, 2009, 325 (5948), pp.1693-1696. ⟨10.1126/science.1173759⟩
Science, American Association for the Advancement of Science, 2009, 325 (5948), pp.1693-1696. ⟨10.1126/science.1173759⟩
Character Transplant When engineering bacteria, it can be advantageous to propagate the genomes in yeast. However, to be truly useful, one must be able to transplant the bacterial chromosome from yeast back into a recipient bacterial cell. But becaus
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0bb7e8f2b606bfc8b269929b001cd3a4
https://hal.inrae.fr/hal-03326000
https://hal.inrae.fr/hal-03326000
Autor:
Kevin C. Axelrod, Jayshree Zaveri, Michael G. Montague, Daniel G. Gibson, Gwynedd A. Benders, Hamilton O. Smith, Clyde A. Hutchison, Mikkel A. Algire, J. Craig Venter, Monzia Moodie
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 105(51)
We previously reported assembly and cloning of the synthetic Mycoplasma genitalium JCVI-1.0 genome in the yeast Saccharomyces cerevisiae by recombination of six overlapping DNA fragments to produce a 592-kb circle. Here we extend this approach by dem
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31275470c6354462651012c3b67b1f95
https://pubmed.ncbi.nlm.nih.gov/19073939
https://pubmed.ncbi.nlm.nih.gov/19073939
Autor:
William G. Farmerie, K. Hlavinka, Meghan May, Vanya Paralanov, Robin H. Miller, J. Jackson, Julie A. Johnson, B. Szczypinski, Jessica B. Hostetler, John I. Glass, Diana Radune, A. S. Durkin, Daniel R. Brown, Gwynedd A. Benders
Publikováno v:
Journal of Bacteriology. 193:2892-2893
Mycoplasma alligatoris and Mycoplasma crocodyli are closely related siblings, one being highly virulent and the other relatively attenuated. We compared their genomes to better understand the mechanisms and origins of M. alligatoris ' remarkable viru
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bac07075433ccf5ffad2e6c9ae7324ce
https://doi.org/10.1128/jb.00309-11
https://doi.org/10.1128/jb.00309-11