Zobrazeno 1 - 10
of 32
pro vyhledávání: '"Guy Girault"'
USE OF HPLC FOR THE STUDY OF ADP BINDING TO CHLOROPLAST ATPase. II. ITS EFFECT ON ENZYMATIC ACTIVITY
Publikováno v:
Journal of Liquid Chromatography & Related Technologies. 23:1639-1655
The binding of ADP to chloroplast ATPase (CF1) has been measured by the chromatographic method of Hummel and Dreyer which allows low affinity binding determinations. Besides about 1.5 endogenous ADP molecules that are irreversibly bound to CF1 or slo
Publikováno v:
Journal of Bioenergetics and Biomembranes. 30:543-553
The fundamental question of the cooperativity between the enzymatic sites of F1-ATPase is examined in the light of new measurements of the enzymatic rate of ATP hydrolysis by CF1, the enzyme isolated from spinach chloroplasts. The experimental data,
Publikováno v:
Photosynthesis Research. 57:253-266
The binding of nucleotides on isolated subunits as well as on reconstituted CF1 core complex is reviewed. Nucleotide interaction with CF1 and consequent ATPase activity are always associated with the presence of Mg2+. The metal binding site studies u
Autor:
Guy Girault, Ralf M. Lösel, John G. Wise, Pia D. Vogel, Gerard Berger, Annette H Erbse, Jürgen H Nett
Publikováno v:
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 52:73-83
The relative structure and binding properties of nucleotide binding sites of the latent, nonactivated chloroplast F1(CF1)ATPase have been investigated by employing ESR spectroscopy using 2-N3-2′,3′-SL-ATP (2-N3-SL-ATP), a spin-labeled photoaffini
Publikováno v:
Biopolymers. 36:135-152
The conformations of the phytotoxic cyclic tetrapeptide tentoxin [cyclo-(L-MeAla1-L-Leu2-MePhe[(Z) delta]3-Gly4)] have been studied in aqueous solution by two-dimensional proton nmr at various temperatures. Contrary to what is observed in chloroform,
Autor:
Stéphane Pezennec, Guy Girault, G. Berger, Sandra Andrianambinintsoa, Nicolas Radziszewski, Edmund Bäuerlein, Jean Michel Galmiche
Publikováno v:
Biochimica biophysica acta (BBA)-Bioenergetics
Biochimica biophysica acta (BBA)-Bioenergetics, Elsevier, 1995, 1231 (1), pp.98-110. ⟨10.1016/0005-2728(95)00071-p⟩
Biochimica biophysica acta (BBA)-Bioenergetics, 1995, 1231 (1), pp.98-110. ⟨10.1016/0005-2728(95)00071-p⟩
Biochimica biophysica acta (BBA)-Bioenergetics, Elsevier, 1995, 1231 (1), pp.98-110. ⟨10.1016/0005-2728(95)00071-p⟩
Biochimica biophysica acta (BBA)-Bioenergetics, 1995, 1231 (1), pp.98-110. ⟨10.1016/0005-2728(95)00071-p⟩
ATPase activity of the F 1 -ATPase from the thermophilic Bacillus PS3 (TF 1 ) was measured as a function of ATP concentration at three different magnesium ion concentrations. A high-performance chromatographic method was used to determine directly AD
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1184:202-206
A simple and rapid procedure has been developed to isolate the subunit from chloroplast coupling factor 1 involving anion exchange HPLC. The β subunit was mainly in the monomeric form, along with some quantities of trimer and more complex aggregates
Publikováno v:
Journal of Liquid Chromatography. 13:4067-4080
The determination by HPLC of released ADP from ATP by chloroplast ATPase (CF1) is described. The enzymatic rate measured by this method is well defined, over several minutes. In the case of ∊ subunit-depleted CF1 or activated CF1, the rate is propo
Publikováno v:
Microbes and Infection
Microbes and Infection, Elsevier, 2000, 2 (1), pp.33-8. ⟨10.1016/S1286-4579(00)00288-4⟩
Microbes and Infection, Elsevier, 2000, 2 (1), pp.33-8. ⟨10.1016/S1286-4579(00)00288-4⟩
International audience; Yellow fever (YF) vaccine using the 17D strain of YF attenuated virus has been produced at the Institut Pasteur in Dakar since 1962. Until now, the stabilised YF had an expiry date of utilization of two years from the end of t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c9f4e762541cf1229c7834eb06ce1eb
https://hal-riip.archives-ouvertes.fr/pasteur-00566865
https://hal-riip.archives-ouvertes.fr/pasteur-00566865
Publikováno v:
Journal of Liquid Chromatography and Related Technologies
Journal of Liquid Chromatography and Related Technologies, Taylor & Francis: STM, Behavioural Science and Public Health Titles, 1998, 21 (13), pp.1925-1955
Journal of Liquid Chromatography and Related Technologies, Taylor & Francis: STM, Behavioural Science and Public Health Titles, 1998, 21 (13), pp.1925-1955
The advantages of the use of HPLC for the purification of the activated chloroplast F1 ATPase, for the measurement of enzymatic activity (by ADP-ATP separation on anion exchange column) and for ATP binding measurement (by the Hummel and Dreyer chroma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba58b88d9e31d071a4a6c5fd71bf8a48
https://hal.inrae.fr/hal-02688572
https://hal.inrae.fr/hal-02688572